ABSTRACT
The prevalence of age-related macular degeneration (AMD) is increasing yearly,and AMD has become the first cause of elderly vision irreversible loss.In China,an aging society is developing,so the prevention and therapy for AMD is faced with a new challenge.Thc multi-faceted study on AMD has become a focus of fundus disease in ophthalmology.AMD is thought to be a multifactorial-induced disease,and it is closely related to age,genetic and environmental factors.But its pathogenesis is not understood enough up to now.In recent years,epidemiological study revealed that the chromosome 10q26 has multiple polymorphic loci.Age-related maculopathy susceptibility 2 ( ARMS2,rs10490924 ) is reported to be a major risk factor of AMD in the yellow race.In this paper,the ARMS2 gene epidemiology,cell positioning and related treatment were reviewed.
ABSTRACT
Background Researches determined that the alteration of A69S locus of age-related maculopathy susceptibility 2 ( ARMS2 ) gene is closely associated with the pathogenesis and progression of age-related maculopathy ( AM D ).However,the location of ARMS2 protein in normal eye tissue is still in controversy,therefore,its function is below understanding up to now.Objective The goal of this laboratory work was to investigate the distribution,expression and location of ARMS2 protein in normal adult retina and choroid as well as in retinal pigment epithelial (RPE) cells and lay a basis for exploring further its function in the protein level.Methods Ten donor eyeballs of normal adult male with the age from 28-42 years were collected in eye bank of Qingdao Eye Hospital.The frozen sections of the retina and choroid were prepared for the detection and location of ARMS2 in 3 eyes by immunofluorescence under the confocal laser microscope.The retina was isolated for the primary culture of RPE cells using explant culture method.The cells were then identified by CK32 antibody by immunofluorescence.The distribution and expression of the ARMS2 protein in retina,ehoroid and RPE cells were determined by immunofluorescence technique.Results ARMS2 protein was strongly expressed in retinal vessel,RPE cell layer,Bruch membrane and choroidal vessel,but weak expression was in retinal ganglion cell layer,inner nuclear layer,outer plexiform layer,outer nuclear layer and inner plexiform layer in the normal eyes.The primarily cultured cells appeared the polygon shape with the abundant pigment in cytoplasm.The immunofluorescence of the cells showed the positive response for CK32,exhibiting the green fluorescence granules in the cytoplasm.The positive expression of ARMS2 protein also was seen in the cytoplasm of RPE cells,appearing the red fluorescence.Conclusions ARMS2 protein mainly distribute and locate retinal and choroidal vessels,RPE cells and Bruch membrane in normal eye.