ABSTRACT
Objective: To investigate the chemical constituents in whole plant of Ainsliaeafragrans. Methods: Silica gel, Sephadex LH-20 gel column chromatography, and semi-preparative HPLC were used to separate and purify the chemical constituents of ethanol extract. And the structures were elucidated on the basis of NMR and MS spectroscopic data. Results: Nine compounds were isolated and identified as zaluzanin C-3-O-β-glucopyranosyl-(1→3)-β-glucopyranosyl-(1→3)-β-glucopyranosyl-(1→3)-β-glucopyranoside (1), macrocliniside B (2), macrocliniside I (3), isochlorogenic acid C (4), 4,5-O-di-caffeoylquinic acid methyl ester (5), isochlorogenic acid A (6), 3,5-O-di-caffeoylquinic acid methyl ester (7), 3,4-O-di-caffeoylquinic acid methyl ester (8), and astragalin (9). Conclusion Compound 1 is a new guaiane sesquiterpene glycoside named as ainslifragsideA.Compounds2, 3, 5, 7, and 8are isolated from this plant for the first time.