ABSTRACT
Objective: The genetic diversity of Akebia trifoliate germplasm from six different populations in Qinling Mountains was studied by AFLP molecular marker. Methods: The amplification bands were obtained by using 10 primer combinations and genetic diversity were estimated for 111 A. trifoliate germplasm. Results: A total of 221 clear bands were obtained, of which 132 were polymorphic bands and the percentage of polymorphic was 59.7%. Among the six populations, the genetic differentiation coefficient was 0.505, gene polymorphism index was 0.185. The six natural populations can be divided into four groups at Nei's genetic distance of 0.13. Conclusion: The populations of A. trifoliate in Qinling Mountains of Shaanxi exist a low level of genetic diversity, almost the same genetic variation occurs within and among populations.
ABSTRACT
Objective: To establish and optimize the AFLP reaction system for genetic diversity analysis on Akebia trifoliata. Methods: The leaves of A. trifoliata were used to analyze the influencing factors of the ligation, the pre-amplification, and the selective amplification of AFLP, so that the optimal AFLP reaction system could be built up. Results: The optimal AFLP reaction system has been set up: added 10 pmo1 of EcoR I adapter, 100 pmol of Mse I adapter, and 2 U of T4 DNA ligase in the ligation mixture; The pre-amplification mixture (20 μL) contained 2. 0 μL of ligation products, 100 ng of each pre-selective primer, E-0 and M-0, 0. 250 mmol/L of dNTPs, 1. 875 mmol/L of Mg2+, 2 U of Taq DNA polymerase; the selective amplification mixture (20 μL) contained 5 μL of 1 : 100 diluted pre-amplification products, 50 ng of each selective primer, E3, M3, 0. 250 mmol/L of dNTPs, 1. 875 mmol/L of Mg2+, 1. 5 U of Taq DNA polymerase. Conclusion: The study on A. trifoliata could establish and optimize the AFLP reaction system which can be used in the research of genetic diversity of the species in future.
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Objective To study the identification method of Akebia trifoliata(Thunb.) Koidz. by Fourier Transform Infrared (FTIR) Spectroscopy. Methods FTIR Spectroscopy was measured of Akebia trifoliate collected from different production areas. Results At the range of 737-1032cm-1, the Spectroscopy of Akebia trifolia of different production areas showed variances in peak value of infrared absorption, peak position, peak shape and peak strength, which can be regarded as identification evidence for Akebia trifoliate. Conclusion This mehthod is rapid, reliable, simple and effective. FTIR can be used as the identification index for Akebia trifoliate.
ABSTRACT
Objective To investigate the effects of seed quality on the using value of Akebia trifoliata. Methods The seed net weight and plumpness were determined by electric balance, the moisture content was determined by moisture meter and the germination rate were determined through germination test. Results The seed net weight degree, 100-grain weight, 1 000 -grain weight, moisture content, viability, germination rate, and germination potential of A. trifotiata fresh seed were 98.7%, 6.64 g, 66.6 g, 43.1%, 94.5%, 82.6%, and 52.8%, respectively. Being made air-dried for 10 d or 60 d, the moisture content of A. trifoliata seeds were 28.5% and 7.8%, respectively, the germination rate were 64.3% and 14.7% respectively. Conclusion It is suitable to put the A. trifoliata seeds into the 30 ℃ clear water for 24 h and make them sprout or be buried in the wet sand at a room temperature of 5-12 ℃. When the moisture content of seed is lower than 8%, it will lose the value to be cultivated.