ABSTRACT
Background: During endodontic intervention, major efforts are made to eliminate bacteria from the infected root canal system. Along these lines, keeping up the chain of asepsis is critical to anticipate sterile root canal system. Currently, gutta-percha (GP) is the most commonly used root canal core filling material. Objective: The purpose of this study was to compare the efficacy of 1% Alexidine, 2% Chlorhexidine, 5.25% Sodium Hypochlorite and saline against E.faecalis in disinfecting gutta-percha cones and to analyze the surface topography of gutta-percha cones after the rapid chemical disinfection procedure. Materials and methods: Gutta-percha cones were contaminated with E.faecalis and immersed in 1% ALX, 2% CHX, 5.25% NaOCl and 0.9% saline for 30 and 60 sec. The disinfected cones were immersed in test tubes and incubated at 37°C for 72 hours. Bacterial growth was evaluated by the presence of turbidity in the broth. Results were confirmed by sub culturing the bacterial colony. For topographical examination, the gutta-percha cones were analyzed under Scanning electron microscopy (SEM). Data was statistically analyzed using one way ANOVA. Swati Srivastava. Rapid chairside disinfection and surface alterations of gutta-percha cones with 1% Alexidine, 2% Chlorhexidine and 5.25% sodium hypochlorite against Enterococcus faecalis - An in vitro comparative study. IAIM, 2019; 6(10): 1-7. Page 2 Results: ALX and NaOCl did not show growth of the tested microorganism for any observed period. CHX completely eliminated E. faecalis after 60 sec. Saline showed no antimicrobial activity. The results of SEM showed some deposits after disinfection procedure by NaOCl and CHX test solutions used. Conclusion: ALX and NaOCl were found to be the most efficient disinfecting agent in eliminating E.faecalis at different time intervals tested. The topographical examination revealed that ALX and CHX left little residue than as compared to NaOCl after 1 minute.
ABSTRACT
Context: The present study was aimed to explore new irrigating solutions, which would probably be as effective as sodium hypochlorite. Aims: The aim of this in vitro study was to evaluate the antibacterial effectiveness of 0.1% octenidine, SmearOFF, 1% alexidine and 5.25% sodium hypochlorite against E. faecalis biofilm formed on tooth substrate. Materials and methods: Eighty extracted human teeth were decoronated, biomechanically prepared, vertically sectioned, placed in the tissue culture wells exposing the root canal surface to E. faecalis strain (ATCC 29212) to establish 3-week-old biofilm. At the end of the 3rd week all groups were treated for 10 minutes with 3 ml of test solutions. Samples exposed to 5.25% sodium hypochlorite (NaOCl) were used as control. All the samples were evaluated for E. faecalis growth and number of colony forming units. Statistical analysis was performed by using one-way analysis of variance (ANOVA) to measure the mean values. The intergroup comparison was done by Tukey HSD post hoc test (p<0.05). Results: Maximum inhibition was observed with 0.1% OCT (group 1) followed by NaOCl (group 4) and ALX (group 3). SmearOFF (group 2) showed the least inhibitory effect. NaOCl (group 4) and ALX (group 3) showed no statistically significant difference (p>0.05), whereas all the other inter‐ group differences were statistically significant (p<0.05). Conclusions: 0.1% OCT showed significantly higher antibiofilm effects. No significant differences Swati Srivastava. Antibiofilm efficacy of 0.1% Octenidine, SmearOFF, 1% Alexidine and 5.25% Sodium Hypochlorite against E. faecalis biofilm formed on tooth substrate. IAIM, 2019; 6(11): 1-8. Page 2 were observed between 1% ALX and 5.25% NaOCl. SmearOFF showed significantly lower antibiofilm effects (p<0.05)
ABSTRACT
The purposes of this study were firstly to investigate the any formation of precipitate after interaction between ALX and NaOCL and secondarily to analyze the PCA formation by using time of flight secondary ion mass (TOF-SIM) spectrometry. Mass spectrometry analysis was performed for the mixture of 0.5% ALX and 5.25% NaOCl. As controls, 2.5% CHX with 5.25% NaOCl and 1% PCA solutions were used. Any formation of precipitates in 10 tested solutions was evaluated by naked eye. Results of mass spectrum showed that the typical peak of PCA was not detected in mixed solution of ALX and NaOCl, whereas CHX/NaOCl mixture showed the same peak that found in the PCA spectrum. Precipitate formation was only observed in CHX/NaOCL mixture. The present TOF-SIM spectrometry results indicated that ALX can be a useful root canal irrigant combined with NaOCl during canal instrumentation. Further study is necessary to confirm the antimicrobial effect of ALX against endodontic pathogen before its clinical application as an endodontic irrigant.