Effect of bone peptide injection in patients with osteoarthritis of the activity of alkaline phosphatase / 中国生化药物杂志

Objective To analyze the effect of bone peptide injection in patients with osteoarthritis of the activity of alkaline phosphatase. Methods A total of 74 patients with osteoarthritis of the bone were selected from March 2015 to May 2017 in our hospital and were randomly divided into two groups,with 37 cases in each group.The control group was treated with glucosamine sulfate, the observation group was treated with ossotide injection.The therapeutic effect and alkaline phosphatase activity were compared between two groups. Results The total effective rate was 97.30% in the observation group, which was significantly higher than that in the control group (70.27%) (P<0.05) .The score of knee function was (79.62±5.80) in the observation group.which was significantly higher than that in the control group (71.06±5.41) (P<0.05).The alkaline phosphatase activity after the treatment of 30d and 45d (0.97±0.12), nkat/L (1.39±0.12) nkat /L was significantly higher than the control group (0.71±0.10), nkat/L, (0.88±0.10) nkat/L, the incidence rate of adverse reaction(5.41 %) was significantly lower than the control group (24.32 %) (P<0.05). Conclusion Bone peptide injection has significant therapeutic effect on bone and joint osteoarthritis, can effectively improve the knee joint function of the patients, improve the patients with alkaline phosphatase activity, and high safety, is worthy of promotion.

Effect of bone peptide injection in patients with osteoarthritis of the activity of alkaline phosphatase / 中国生化药物杂志

Objective To analyze the effect of bone peptide injection in patients with osteoarthritis of the activity of alkaline phosphatase. Methods A total of 74 patients with osteoarthritis of the bone were selected from March 2015 to May 2017 in our hospital and were randomly divided into two groups,with 37 cases in each group.The control group was treated with glucosamine sulfate, the observation group was treated with ossotide injection.The therapeutic effect and alkaline phosphatase activity were compared between two groups. Results The total effective rate was 97.30% in the observation group, which was significantly higher than that in the control group (70.27%) (P<0.05) .The score of knee function was (79.62±5.80) in the observation group.which was significantly higher than that in the control group (71.06±5.41) (P<0.05).The alkaline phosphatase activity after the treatment of 30d and 45d (0.97±0.12), nkat/L (1.39±0.12) nkat /L was significantly higher than the control group (0.71±0.10), nkat/L, (0.88±0.10) nkat/L, the incidence rate of adverse reaction(5.41 %) was significantly lower than the control group (24.32 %) (P<0.05). Conclusion Bone peptide injection has significant therapeutic effect on bone and joint osteoarthritis, can effectively improve the knee joint function of the patients, improve the patients with alkaline phosphatase activity, and high safety, is worthy of promotion.

Effects of salvianolic acid B on osteogenic differentiation of human periodontal ligament cells / 华西口腔医学杂志

<p><b>OBJECTIVE</b>This study investigated the effects of salvianolic acid B (Sal B), a major bioactive component of the Chinese medicine salvia miltiorrhiza, on osteogenic differentiation of human periodontal ligament cells (hPDLCs).</p><p><b>METHODS</b>Third passage PDLCs were used in this experiment. Methyl thiazolyl tetrazolium (MTT) method was employed to observe the effects of different Sal B concentrations on proliferation activity of hPDLCs. Alkaline phosphatase (ALP) activity and mineralization capability were measured, and mRNA expression of osteocalcin (OCN) was detected to investigate the effects of Sal B on osteogenesis of hPDLCs.</p><p><b>RESULTS</b>Sal B did not influence the viability of hPDLCs. The ALP activity and OCN mRNA expression levels of hPDLCs were both significantly improved (P<0.05) under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) compared with those in OIM group. Moreover, the number of mineralized nodules formed by hPDLCs were considerably higher under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) than that in the OIM group.</p><p><b>CONCLUSIONS</b>Appropriate Sal B concentration can improve the osteogenic differentiation of hPDLCs.</p>

A comparative analysis of basic characteristics of several deproteinized bovine bone substitutes / 대한치주과학회지

PURPOSE: Deproteinized bovine bone substitutes are commonly used in dental regenerative surgery for treatment of alveolar defects. In this study, three different bovine bone minerals - OCS-B (NIBEC, Seoul, Korea), Bio-Oss (Geistlich - Pharma, Switzerland), Osteograft/N - 300 (OGN, Dentsply Friadent Ceramed. TN, USA) - were investigated to analyze the basic characteristics of commercially available bone substitutes. METHODS:Their physicochemical properties were evaluated by scanning electron microscopy, energy dispersive X-ray spectrometer (EDS), surface area analysis, and Kjeldahl protein analysis. Cell proliferation and alkaline phosphatase (ALP) activity of human osteosarcoma cells on different bovine bone minerals were evaluated. RESULTS: Three kinds of bone substitutes displayed different surface properties. Ca/P ratio of OCS - B shown to be lower than other two bovine bone minerals in EDS analysis. Bio-Oss had wider surface area and lower amount of residual protein than OCS - B and OGN. In addition Bio - Oss was proved to have lower cell proliferation and ALP activity due to lots of residual micro particles, compared with OCS - B and OGN. CONCLUSIONS: Based on the results of this study, three bovine bone minerals that produced by similar methods appear to have different property and characteristics. It is suggested that detailed studies and quality management is needed in operations for dental use and its biological effects on new bone formation.

Effect of Extracts from Safflower Seeds on Osteoblastic Differentiation and Intracellular Free Calcium Concentration in MC3T3-E1 Cells

Very little research has been carried out on safflower seed for the prevention and treatment of the bone deficiency diseases, including osteoporosis, which are supported by scientific evidences. In the present study, 3microliter of 0.1% dried crude extract or 2microliter of 0.1% dried aqueous fraction were shown to significantly accelerate the rate of differentiation of osteoblast. Also, the crude extract and aqueous fraction increased the [Ca2+]i of the cultured osteoblast cells: 3microliter of 0.1% dried crude extract and 2microliter of 0.1% dried aqueous fraction significantly increased the [Ca2+]i of the cultured osteoblast cells (8x104) to the extent that it deserves a considerable attention. Furthermore, the crude extract and aqueous fraction increased the [Ca2+]i of the cultured osteoblast cells, and 300microM Cd2+, specific calcium channel blocker, completely blocked the increase. Therefore, the increased [Ca2+]i of the cultured osteoblast cells by safflower seed component continued to activate calcium channel.

Effect of titanium surface roughness on adhesion and differentiation of osteoblasts / 대한치주과학회지

The success of an implant is determined by its integration into the tissue surrounding the biomaterial. Surface roughness is considered to influence the behavior of adherent cells. The aim of this in vitro study was to determine the effect of surface roughness on Saos-2 osteoblast-like cells. Titanium disks blasted with 75 micrometer aluminum oxide particles and machined titanium disks were prepared. Saos-2 were plated on the disks at a density of 50,000 cells per well in 48-well dishes. After 1 hour, 1 day, 6 days cell numbers were counted. One day, 6 days after plating, alkaline phosphatase(ALPase) activity was determined. Compared to experimental group, the number of cells was significantly higher on control group. The stimulatory effect of surface roughness on ALPase was more pronounced on the experimental group than on control group. These results demonstrate that surface roughness alters proliferation and differentiation of osteoblasts. The results also suggest that implant surface roughness may play a role in determining phenotypic expression of cells.

Osteoblast adhesion and differentiation on magnesium titanate surface / 대한치주과학회지

The nature of the implant surface can directly influence cellular response, ultimately affecting the rate and quality of new bone tissue formation. The aim of this in vitro study was to investigate if human osteoblast-like cells, Saos-2, would respond differently when plated on disks of magnesium titanate and machined titanium. Magnesium titanate disks were prepared using Micro Arc Oxidation(MAO) methods. Control samples were machined commercially pure titanium disks. The cell adhesion, proliferation and differentiation were evaluated by measuring cell number, and alkaline phosphatase(ALPase) activity at 1 day and 6 day after plating on the titanium disks. Measurement of cell number and ALPase activity in Saos-2 cells at 1 day did not demonstrate any difference between machined titanium and magnesium titanate. When compared to machined titanium disks, the number of cells was reduced on the magnesium titanate disks at 6 day, while ALPase activity was more pronounced on the magnesium titanate. Enhanced differentiation of cells grown on magnesium titanate samples was indicated by decreased cell proliferation and increased ALPase activity.

The effect of rhBMP-2 on delta12-PGJ2 induced osteoblastic differentiation and mineralization / 대한치주과학회지

Prostaglandin plays a significant role in the local control of bone metabolism associated with periodontal disease. delta12-PGJ2 is a natural PGD2 metabolite that is formed in vivo in the presence of plasma. It is known for delta12-PGJ2 to stimulate calcification in osteoblastic cells. Bone morphogenetic protein(BMP) stimulated osteoblastic differentiation in various types of cells and greatly enhanced healing of bony defects. The purpose of this study was to evaluate the effect of rhBMP-2 on delta12-PGJ2 induced osteoblastic differentiation and mineralization in vitro. A human osteosarcoma cells line Saos-2 were cultured. In the test groups, 10-7M of delta12-PGJ2 or mixture of 10-8M of delta12-PGJ2 and 100ng/ml of rhBMP-2 or 100ng/ml of rhBMP-2 were added to culture media. After 1 day, 2 days and 4 days of culture period, the cell number was measured. Alkaline phosphatase activity was measure at 3 days. Reverse transcription polymerase chain reaction(RT-PCR) was performed to determine the expression of mRNA of bone matrix protein at 8 hours, 1 day and 7 days. The ability to produce mineralized nodules in rat osteoblasts(MC3T3-E1) was evaluated at 21 days. The results were as follows : 1. rhBMP-2 or mixture of rhBMP-2 and delta12-PGJ2 inhibited cell proliferation of human osteosarcoma cells. 2. rhBMP-2 or mixture of rhBMP-2 and delta12-PGJ2 stimulated alkaline phosphatase activity significantly higher than delta12-PGJ2 alone. 3. rhBMP-2 or mixture of rhBMP-2 and delta12-PGJ2 stimulated mineralization compared to delta12-PGJ2 alone. 4. mRNA of alkaline phosphatase, BMP-2, cbfa 1, Type I collagen were detected in the group treated with delta12-PGJ2/rhBMP-2, rhBMP-2 alone, delta12-PGJ2 alone. These results show that mixture of delta12-PGJ2 and rhBMP-2 causes more bone formation than delta12-PGJ2 alone while the bone formation effects of mixture of delta12-PGJ2 and rhBMP-2 are less than those of rhBMP-2 alone. Further researches would be necessary to clarify the interactions of these agents.

Effects of ginsenoside Rg_(1) , chitosan and TGF-?_(1) on the proliferation and differentiation of human periodontal ligament cells / 医学研究生学报

Objective: To observe the effects of ginsenoside Rg_(1) , chitosan and TGF-?_(1) on the proliferation and differentiation of human periodontal ligament cells( PDLC). Methods:Human periodontal ligament cells were isolated and cultured. The effects of ginsenoside Rg_(1)(0.01 ?mol/L), chitosan((0.05 g/L,)0.1 g/L, 0.2 g/L) and TGF-?_(1)(0.5 ?g/L, 1 ?g/L, 2 ?g/L) on the proliferative ability of human PDLCs were evaluated with MTT method. The alkaline phosphatase activities of human PDLCs were measured with spectrophotometric assay. The secretion of osteocalcin of human PDLCs were measured with radioimmunological method and the apotosis rates of human PDLCs were assayed with flow cytometry with PI staining method. Results: ①Comparing with the control group, the proliferative ability of human PDLCs in ginsenoside Rg_(1)(0.01 ?mol/L)group ,Chi(0.05 g/L, 0.1 g/L, 0.2 g/L) groups and TGF-?_(1 )((0.5 ?g/L), 1 ?g/L, 2 ?g/L) groups on day 3,5,7 were considerably increased (P

The Effect of Cyclic Compressive Strain on the Differentiation of Osteoblast-Like Cells (MC3T3-E1) in 3-D Scaffold / 대한정형외과연구학회지

We examined the effect of an applied cyclic compressive strain on the growth and differentiation of MC3T3-E1 cultured in a three-dimensional chitosan scaffold. The specially designed testing apparatus for mechanical stimulus was developed for uniaxial cyclic compressive strain. Cyclic compressive strain was applied over a period of 17 days with 150 cycles per day at a frequency of 0.5hz. Strain magnitude was 2.5% of the scaffold length. Control group and mechanically stimulated group were incubated and harvested at the indicated times. (day 3, 7, 10, 14, 17) The total amount of protein and alkaline phosphatase activity were examined. The total amount of protein of the control group was higher than that of the mechanically stimulated group. This was due to cell death for the nodule formation and calcium deposit of the mechanical stimuli group which resulted in cell differentiation. The alkaline phosphatase activity increased slightly in the control group. However, in the mechanical stimuli group, it increased significantly and reached its peak level on day 7 and subsequently its activity dropped to a level that was higher than the level at day 4(p < 0.05). Conclusively, it can be noted that the mechanical stimulus significantly accelerated the differentiation of MC3T3-E1 cells.

Effects of IL-1 ? on the Biological Activity of Human Periodontal Ligament Cells in Vitro / 中国医师杂志

Objective To explore the effects of interleukin- 1?(IL-1?)on the biological activity of human periodontal ligament(PDL) cells in vitro. Methods Human PDL cells were cultured in DMEM medium containing IL-1?(0.1,0.5,1,5 and 10ng/ml) for 1,2,3,4 and 5 days, respectively. The proliferation of PDL cells was measured by MTT assay at the first, second, third, fourth and fifth days after IL-1? treatment, respectively. Fibronectin level in the medium was determined by ELISA at the fourth days after 10ng/ml IL-1? treatment, and alkaline phosphatase(ALP) activity was measured by enzyme kinetic method. Results IL-1? inhibited the growth of human PDL cells in a dose-dependent manner, and its lowest effective concentration was 1ng/ml(P

The Effect of Hematopoietic Stem Cell Transplantation in the Origin and the Osteoblastic Differentiation of the Human Bone Marrow Stromal Cell / 대한내분비학회지

BACKGROUND: Bone marrow transplantation is the treatment of choice for patients with certain- hematological malignancies, many of whom will survive many years thereafter. Bone disease is a potential longterm complication. But, little is known about the effects of bone marrow transplantation on bone. METHODS: In this study, bone marrow was obtained from healthy donor and transplant recipients. Then mononuclear cells including marrow stromal cells were isolated and cultured. At near confluence, bone marrow stromal cells were subcultured. Thereafter alkaline phosphatase activities of each group were measured by time course of secondary culture. We also analysed the origin of marrow stromal cells by the polymerase chain reaction using YNZ 22 minisatellite probe. RESULTS: l. Cells cultured in our system showed the characteristics of marrow stromal cells differentiated to osteoblasts. They were in fibroblast-like spindle shape and positive to alkaline pbosphatase histochemistry and Von Kossa histochemistry in secondary cultures. 2. The time required for the near confluence in the primary culture was 15 days and 22.9 days on the average in healthy donors and transplant recipients, respectively (p=0.003). 3. In secondary cultures, healthy donors and transplant recipients showed peak alkaline phosphatase activity at 10 days and 17 days, respectively (p=0.031). Alkaline phosphatase activity was lower in BMT recipients than in healthy donors during the whole period of secondary cultures. 4. In polymerase chain reaction analysis using YNZ 22 minisatellite probe, bone marrow stromal cells were of recipient origin. CONCLUSION: Recipient-derived bone marrow stromal cells may be damaged secondary to the effect of chemotherapy, glucocorticoid & total body irradiation which have given before bone marrow transplantation. So it may affect the differentiation of bone marrow stromal cells into the osteoblasts.

The effects of continuous and intermittent compressive pressure on alkaline phosphatase activity of periodontal ligament cells / 대한치과교정학회지

The propose of this study was to evaluate the effect of cellular activity of PDL cells dependent on intermittent and continuous compressive force by determining the alkaline phosphatase activity. An intermittent and continuous compressive forces were applied on PDL cells at the confluent stage. The alkaline phosphatase activity was measured on control and experimental groups every 24, 48, 72hours. The experimental group were consist of continous and intermittent compressive group which were compressed by 300g/cm2 of diaphragm pump. The intermittent compressive group was connected by timer which was worked on 10 minutes and off 10minutes. The results were as follow; 1. The alkaline phosphatase activity of intermittent compressive group was lower than control group at 24 hours(P<0.05). 2. The alkaline phosphatase activity between each groups showed no significant difference at 48hours. 3. The alkaline phosphatase activity of continuous compressive group was significantly higher than control group at 72 hours(P<0.01).

The effects of mechanical stress on alkaline phosphatase activity of MC3T3-E1 cells / 대한치과교정학회지

Orthodontic force is a mechanical stress controlling both of tooth movement and skeletal growth. The mechanical stress stimulate bone cells that may exert some influence on bone remodeling. The purpose of this study was to evaluate the difference in cellular activity depending on mechanical stresses such as compressive and tensile force by determining the alkaline phosphatase(ALP) activity. A clonal osteogenic cell line MC3T3-E1 was seeded into a 24-well plate(2x 10(4)/well). At the confluent phase, a continuous compressive hydrostatic pressure(25g/cm2, 300g/cm2) and continuous tensile hydrostatic pressure( -25g/cm2, -300g/cm2) were applied for 4, 6, 10, 14, 18, 20 days respectively by a diaphgragm pump. At the end of the stimulation period, cell layers were prepared for ALP activity assay. The ALP activity of the compressive group increased more than that of the tensile group at same force magnitude, whereas the cells responded to a similar pattern regardless of the type of mechanical stress. The ALP activity of the compressive and tensile group turned into the level of the control group as the length of time increased. These results indicated that a mechanical stress may be more effective on cellular activity during active cellular proliferation and differentiation periods. The time to achieve maximum ALP activity was delayed as the mechanical stress increased in both the compressive and the tensile group. Accordingly, the magnitude of the stress rather than the type of mechanical stress may have more influence on cellular activity.