House Dust Mite Allergic Rhinitis Model in C57BL/6 Mice / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: The animal model of allergic rhinitis is important to study the pathophysiology of allergy and to design an effective therapy to ameliorate allergic diseases. Despite of numerous reported animal models of allergic rhinitis, there were few reports of murine model sensitized with house dust mite, which is one of the most common antigen that induce allergic rhinitis. The aim of this study was the establishment of the murine model for house dust mite allergy. MATERIALS AND METHODS: C57BL/6 mice were sensitized with Dermatophagoides farinae (Der f) crude extract in complete Freund's adjuvant and repetitive intranasal instillation of Der f extract a total of 6 times at 1 week intervals (group A). In some mice, allergen was intranasally instilled at 1 week intervals without sensitization of allergen (group B). RESULTS: After allergen challenge, nasal symptoms were significantly increased in group A mice. Histopathologically, the number of eosinophil in nasal mucosa were also significantly increased in group A and B mice. High level of Der f-specific IgE antibody was observed in group A mice, whereas those of group B mice was low. CONCLUSION: Our findings suggested that house dust mite allergy can be developed by systemic sensitization of Der f extract with adjuvant and intranasal instillation of allergen. This is more effective method than local sensitization of allergen only.

Expression and Role of Adhesion Molecules in the Nasal Mucosa of the Rat with Experimentally Induced Allergic Rhinitis / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: Airway allergic reactions are induced by infiltrating inflammatory cells into the human airway tissues through interactions between vascular endothelial cells, inflammatory mediators and adhesion molecules. Accordingly, it is important to study the role of adhesion molecules for the evaluation of pathophysiology of allergy. The aim of this study is to investigate the effect of monoclonal antibodies against intercellular adhesion molecule-1 (ICAM-1) and leukocyte function associated molecule-1 (LFA-1) on the pathophysiology of allergy in ovalbumin-sensitized rats. MATERIALS AND METHODS: We developed an allergy model in rat using the intraperitoneal injection and intranasal nebulization of ovalbumine solution. We evaluated in vivo effects of ICAM-1 and LFA-1 monoclonal antibodies on the expression of ICAM-1 and LFA-1 in ovalbumin sensitized rats. RESULTS: Nasal symptoms after allergen challenge were significantly suppressed and the number of eosinophil in nasal mucosa were significantly inhibited by the treatment of adhesion molecule antibodies. Anti-ICAM-1 and anti-LFA-1 monoclonal antibodies suppressed the expression of ICAM-1 and LFA-1 in nasal mucosa of ovalbumin-sensitized rats. CONCLUSION: It is suggested that allergy can be managed by a useful treatment method using adhesion molecule antibody.