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Objective To investigate the effect of exogenous vascular endothelial growth factor (VEGF) on bone activity of rabbit heterotopic allograft decalcified bone.Methods 140 adult healthy China white rabbits were selected,no limitation with sex,20 rabbits as the donor preparation of allogenic decalcified bone,according to the random number table,the rest was divided into the experimental group (allograft decalcified bone ± VEGF) and the control group (Allograft decalcified bone),each group contained 60 rabbits.For the experimental group,the prepared 1.5 cm long homologous decalcified tibia was placed in rabbit right thigh of rectus femoris and vastus medialis muscle gap near by saphenous artery,and fixed on the femur with two 0.8 mm Kirschner wire.In the vicinity of the skin,implanted an osmotic pump which contain the VEGF solution 200 μl with concentration was 0.5 μg/ml.In the control group,implanted the isometric allograft decalcified bone in rabbit right thigh corresponding parts with the same method.Each group respectively at 0,2,4,6,8,10 weeks to death 10 white rabbits,By specimen observation,HE dyeing observation and detection of type Ⅰ glue protein fluorescence intensity,Analysis the bone activation degree of two groups of bone allograft decalcified.Results Experimental allograft decalcified bone gradually wrapped by connective tissue membrane,its surface appear different size of the pits and gradually increased and become deep,while the control group pits relatively little and shallow.In the experimental group and control group,the fluorescence intensity of type Ⅰ collagen reached its peak respectively at 8 weeks (47.57 ±3.50) and 10 weeks (45.07±6.02),with no statistically significant (P ＞ 0.05).Conclusion Rabbit allograft decalcified bone implanted in the muscle clearance with abundant blood supply can be transformed into activated bone after 10 weeks,and after applying exogenous VEGF,allograft decalcified bone can be transformed into activated bone after 8 weeks,the bone activation process obviously speed up.The reaults confirmed the exogenous VEGF can obviously promote the ectopic rabbit bone allograft decalcified bone activation process.
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Objective To explore the clinical effect of autogenous bone,allograft bone and BMP synthetic bone in the treatment of lum-bar spondylolisthesis,and provide more basis to choose transplant material.Methods A total of 96 patients with lumbar spondylolisthesis were chosen as research subjects,who were cured in our hospital from January 2014 to January 2015.They were divided into group A(who were treated with autogenous bone),group B(who were treated with allograft bone)and group C(who were treated with BMP synthetic bone), according to prospective study method.The indicators of the operation,postoperative adverse reactions,change of intervertebral disc height and bone graft fusion rate of three groups were compared.Results Difference of the operation indexes of three groups had no statistical sig-nificance(P >0.05).The incidences of adverse reactions in group A and group C had no statistically significant difference(P >0.05),but both less than that in group B,with statistically significant difference(P 0.05),but both more than that in group B,with statistically significant difference(P 0.05).And the fusion rates of group A and C in each period were significantly higher than that of group B,with statistically significant difference(P 0.05),but both better than that of group B,with statistically significant difference (P <0.05).Conclusion BMP synthetic bone used in lumbar spondylolisthesis has the same clinical effect as autologous bone.But BMP synthetic bone has faster bone graft fusion rate than autologous bone.And it is beneficial to patients’recovery.
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El objetivo de este trabajo experimental es determinar si el agregado de plasma rico en plaquetas influye en la osteointegración del aloinjerto crioconservado con antibióticos, en fracturas infectadas, con pérdida ósea, en huesos largos de conejos. Materiales y métodos: Se evaluaron cuatro grupos de 7 conejos cada uno, a los cuales se les generó una fractura infectada del fémur derecho. En los cuatro grupos, se colocó aloinjerto molido con antibiótico local, en el grupo II y en el grupo IV, se agregó también plasma rico en plaquetas. Se sacrificó a los grupos I y II, a los 30 días, y a los grupos III y IV, a los 90 días. Se realizó una evaluación histológica y tomográfica. Resultados: Nueve conejos murieron en el período de estudio. No hubo diferencias estadísticamente significativas entre los grupos evaluados. En el grupo IV, con plasma rico en plaquetas, se observó neoformación ósea en tres de las cuatro muestras; se advirtieron sectores de acoplamiento entre el tejido receptor y el injertado, con consolidación en las imágenes tomográficas. Conclusiones: En este trabajo, hubo correlación entre los resultados histológicos y tomográficos. Pese a que no se hallaron diferencias estadísticamente significativas, el agregado de plasma rico en plaquetas, además de estimular la generación de nuevos tejidos, disminuyó la reabsorción del tejido injertado al análisis anatomopatológico, y mostró mayor neoformación ósea y consolidación en el grupo IV (75 por ciento) tratado con plasma rico en plaquetas que en el grupo III (25 por ciento) al que no se asoció plasma rico en plaquetas al aloinjerto óseo
The objective of this experimental work was to determine whether the addition of platelet-rich plasma influences the osseointegraton of cryopreserved allograft with antibiotics in infected fractures with bone loss, in long bones of rabbits.Methods: Four groups of 7 rabbits with generated infected fracture of the right femur. Milled allograft with local antibiotic was placed in the four groups; groups II and IV also received platelet-rich plasma. Rabbits in groups I and II were sacrificed at 30 days, and those of groups III and IV at 90 days. Histological and CT evaluation was performed.Results: Nine rabbits died during the study period. There were no statistically significant differences between groups. In group IV with platelet-rich plasma, bone formation was observed in three of the four samples, with noticeable areas of coupling between the receiver and the grafted tissue with consolidation in CT.Conclusions: In our study, there was no correlation between the histological and tomographic findings. Although we found no statistically significant differences, the addition of platelet-rich plasma stimulated the generation of new tissue, and also decreased reabsorption of the grafted tissue according to the pathologic analysis. Besides increased bone formation and consolidation was detected in group IV (75%) treated with platelet-rich plasma, compared to group III (25%) without platelet-rich plasma bone allograft.
Subject(s)
Animals , Fracture Healing , Osseointegration , Platelet-Rich Plasma , Bone Regeneration , Transplantation, Homologous/methods , Rabbits , Fractures, Bone/complicationsABSTRACT
Se describe el tratamiento periodontal en un defecto óseo situado mesial a la pieza 2.3 y distal a la pieza 2.2, mediante la terapia combinada de derivado de la matriz del esmalte (DME) y aloinjerto óseo mineralizado. Se valoró la zona afectada y se planeo su abordaje quirúrgico para la terapia combinada. Seis meses después del tratamiento se observó una reducción significativa en la profundidad al sondeo y ganancia en los niveles de inserción, así como el relleno óseo del defecto en la evaluación radiográfica. Los resultados reafirman la efectividad del DME y el aloinjerto óseo mineralizado en la terapia regenerativa periodontal.
We describe the periodontal treatment in a bone defect located mesial to the 2.3 tooth, using combination therapy of enamel matrix derivate (EMD) and mineralized bone allograft. We evaluated the affected area and planned their surgical approach to combination therapy. Six months after treatment it showed a significant reduction in probing depth and gain in attachment levels and bone filling of the defect in the radiographic evaluation. These results confirm the effectiveness of the EMD and the mineralized bone allograft in periodontal regenerative therapy.
Subject(s)
Humans , Female , Middle Aged , Alveolar Bone Loss/surgery , Dental Enamel Proteins/therapeutic use , Bone Transplantation/methods , Bone Regeneration , Combined Modality Therapy , Periodontal Attachment Loss/surgery , Treatment OutcomeABSTRACT
Objective To discuss the design of arthroscopic reconstruction of posterior cruciate ligament (PCL) with quadriceps tendon fixed by a patellar block and an allograft bone plug, and its clinical outcome. Methods Thirteen patients suffering from PCL injury were treated by the technique mentioned above. The connection between quadriceps tendon and superior patellar pole was preserved in the harvest process of quadriceps tendon. The femoral tunnel was made like a bottle neck, which was clogged with the patellar bone block. The quadriceps tendon strip was fixed by an allograft bone plug pressed into the tibial tunnel. Their functional recovery was evaluated by Lysholm scoring and IKDC(International Knee Documentation Committee) criteria. Results In the average follow-up period of 19 months, there were not any severe nerve or blood vessel lesions, or failures of transplanted ligament None of the patient complained of knee instability after the operation. The pain was relieved in most patients, but three complained of pain after knee movement A little calcification was found in the quadriceps tendon at the superior patellar pole one year after the operation in the X-ray of a patient There was significant difference in Lysholm scale between pre-operation and post-operation (P