ABSTRACT
Resumen El objetivo de este trabajo fue obtener un sistema que pueda ser usado para conservar de manera localizada en una herida dérmica las propiedades cicatrizantes y refrescantes del Aloe Vera usado en la medicina tradicional, integrando dicho principio activo en una matriz polimérica compuesta por alginato, quitosano y alcohol polivinílico (PVA), los cuales en conjunto poseen propiedades de resistencia mecánica, biodegradabilildad y biocompatibilidad, necesarios para este tipo de aplicación. Para llevar a cabo esta investigación, se evaluó la morfología, grupos funcionales, propiedades mecánicas y cinética de liberación de las muestras. Cada una de las matrices evidenciaron una morfología con poros interconectados y estabilidad mecánica. La liberación de Aloe vera desde la matriz de PVA-alginato-quitosano tuvo un comportamiento no fickiano, mientras la de alginato-quitosano siguió un comportamiento más cercano al fickiano. Las matrices de PVA-quitosano-alginato y alginato- quitosano por sus características, mecánicas, morfológicas de poros definidos e interconectados, y de cinética de liberación representan buenos modelos para ser tenidos en cuenta como sistemas de liberación controlada de medicamentos.
Abstract The purpose of this work was to obtain polymeric systems from Polyvinyl Alcohol (PVA), alginate and chitosan as possible matrix to encapsulate Aloe vera gel, seeking to integrate mechanical strength, biodegradability and biocompatibility of these polymers. In addition, Aloe vera gives the polymer matrix healing and emollient properties. Morphology, functional groups, mechanical properties and kinetic release behavior of the samples were evaluated. The matrices showed a morphology with interconnected pores and mechanical stability. The release of Aloe vera from the matrix of PVA-alginate-chitosan exhibited a non Fickian behavior, while alginate-chitosan presented a closer to Fickian behavior. PVA-chitosan-alginate and chitosan matrices, by their mechanical, morphological and interconnected and well defined pores, and alginate-release kinetics properties, represent good models to be taken into account as systems of controlled drug release.
Resumo O objetivo deste trabalho foi obter sistemas poliméricos a partir de Polivinil Álcool (PVA), alginato e quitosana como possíveis matrizes para encapsular o gel de Aloe vera, buscando integrar resistência mecânica, biodegradabilidade e biocompatibilidade desses polímeros. Além disso, a Aloe vera confere propriedades de cura e emoliência à matriz polimérica. Morfologia, grupos funcionais, propriedades mecânicas e comportamento de liberação cinética das amostras foram avaliados. As matrizes mostraram uma morfologia com poros interconectados e estabilidade mecânica. A liberação de Aloe vera a partir da matriz de PVA-alginato-quitosana exibiu um comportamento não Fickiano, enquanto alginato-quitosana apresentou um comportamento mais próximo ao Fickiano. As matrizes PVA-quitosana-alginato e quitosana, por seus poros mecânicos, morfológicos e interconectados e bem definidos, e as propriedades cinéticas de liberação de alginato, representam bons modelos a serem considerados como sistemas de liberação controlada do fármaco.
ABSTRACT
Objective To investigate the effect of three kinds of skin external use in the prevention and treatment of radiation dermatitis in patients with nasopharyngeal carcinoma. Methods 120 patients with nasopharyngeal carcinoma who were treated form August 2014 to October 2015 were divided into three groups by random number table method, namely, aloe vera gel group, triethanolamine cream group, radiation treatment of skin protectants (lifuning) group. Except for different medications among the groups, the other conditions remained relatively consistent. 3 groups were compared in the radiotherapy classification, area, local pain score, and topical drug costs during the radiotherapy and the radiation dose when radiation dermatitis occurred the first time. Results Three kinds of skin external use had a preventive effect, but the effect was different. Three groups′ patients with radiation dermatitis incidence was 100%, but occurred at different levels. Aloe vera gel cream group and triethanolamine group were mainly for gradeⅡradiation dermatitis, grade IV radiation dermatitis occurred in 2 cases and 4 cases, lifuning group were mainly for grade Ⅰ and grade Ⅱ radiation dermatitis, grade Ⅲ radiation dermatitis only occurred 2 cases, noⅣgrade radiation dermatitis occurred. Comparison among the three groups, the difference was statistically significant (H=8.758,P0.05). The difference between in lifuning group and triethanolamine cream group in pain had no statistically significant (χ2=2.47,P>0.05), other indexes had significant differences (P 0.05). Conclusions Considering the efficacy, pain and price three aspects which skin external use prevention and treatment radiation dermatitis patients with nasopharyngeal carcinoma, lifuning as the mixture of triethanolamine and aloe can effectively reduce and delay the chance of radiation dermatitis, reduce the radiation field experience of local pain, and affordable, the patients are more acceptable, it′s worthy of clinical use.
ABSTRACT
Objective:To investigate the effects of three herb extracts on cell proliferation and collagen synthesis in cultured hu-man dermal fibroblasts. Methods:The human dermal fibroblasts were isolated and cultured with tissue explant culture technique. Im-munohistochemical staining was performed to observe vimentin in fibroblasts. The proliferation of fibroblasts was determined by MTT as-say. Hydroxyproline in the cultural medium was determined by a digestion method. Results:The cells were typical fibroblasts, and the immunohistochemistry staining of vimentin in the cells was positive. The three herb extracts could promote the proliferation of the fibro-blasts at appropriate concentrations in a time-dependent manner. After the 72-hour culture in the medium, 0. 312 5-5. 000 0 mg·ml-1 Aloe vera gel extract, 0. 156 3-2. 500 0 mg·ml-1 Bletilla striata extract and 0. 075 0 mg·ml-1 salvianolate showed significant effects on the proliferation of fibroblasts (P<0. 05 or P<0. 01 vs the blank group). Compared with that in the blank group, the amount of hydroxyproline in the three herb extracts cultural medium was also increased (P<0. 05 or P<0. 01). The effect of salvianolate on the cell proliferation was closely related with time and concentration, and the high concentration group could inhibit the growth of fibroblasts as the extension of time. Conclusion:Aloe vera gel extract, bletilla striata extract and salvianolate can promote the proliferation of fi-broblasts and the production of hydroxyproline, which may contribute to the wound healing.
ABSTRACT
Administration of a single dose of doxorubicin (DOX) (7.5 mg/kg, iv) produces cardiotoxicity, manifested biochemically by significant decrease in blood glutathione (GSH) and tissue GSH along with elevated levels of serum lactate dehydrogenase (LDH) and serum creatine phosphokinase (CPK). In addition, cardiotoxicity was further confirmed by significant increase in lipid peroxides expressed as malondialdehyde (MDA, secondary indicator of lipid peroxidation), tissue catalase and tissue superoxide dismutase (SOD). Administration of A. vera gel (100 and 200 mg/kg) orally for 10 days produced a significant protection against cardiotoxicity induced by DOX evidenced by significant reductions in serum LDH, serum CPK, cardiac lipid peroxides, tissue catalase and tissue SOD along with increased levels of blood and tissue GSH. The results revealed that A. vera gel produced a dose dependent protection against DOX induced cardiotoxiaty.
ABSTRACT
The present work was undertaken with a view to study the effect of oral feeding of 2% Aloe vera gel extract (AGE) for 30 days on azoxymethane (AOM)-induced oxidative stress in rats. It was observed that AOM administration resulted in a significant increase in malondialdehyde and conjugated dienes, with reduction in hepatic glutathione (GSH), vitamin A and uric acid contents. AOM-induced reduction in hepatic GSH and uric acid was brought back to normal by AGE. There was a significant raise in hepatic catalase, superoxide dismutase and glucose-6-phosphate dehydrogenase (G-6-PD) activities as a result of feeding of the extract. Ingestion of the extract effected reduction in AOM-induced colonic GSH-peroxidase, G-6- PD and glutathione S-transferase and femur bone marrow micronuclei formation. Hence, it is suggested that Aloe vera gel extract possess the ability to reduce AOM- induced oxidative stress and toxicity in liver.
ABSTRACT
BACKGROUND: Aloe vera has been used for medical purposes since antiquity, so it is known as the healing plant or silent healer. It has been claimed that aloe has several important therapeutic properties, including wound healing, thermal injury healing and anti-inflammation and immunomodulation properties. Because of these effects, it has become an ingredient in a wide variety of cosmetic products, including night creams, soaps, shampoos, suntan lotions and cleansers. OBJECTIVE: In order to determine the anti-irritant effect of aloe vera, we compared the degree of stinging intensity caused by 10% lactic acid solution and a mixture of 20% lactic acid and 100% aloe vera. METHODS: We carried out sting tests on 34 volunteers who were all over 20 years of age and they had no past and present history of atopic dermatitis and other eczema. The lactic acid sting test consisted of a 10-minute exposure on the left cheek with 10% lactic acid solution using a Hilltop chamber and the subjects recorded the degree of stinging intensity by themselves every 1 minute during 10-minutes of exposure. We also carried out a sting test with a mixed solution of 20% lactic acid and 100% aloe vera, and we compared the intensity and onset time of the stinging sensation. RESULTS: Aloe vera had some effect to ameliorate the intensity of the stinging sensation and also to delay the time required for the stinging sensation caused by lactic acid. There was a significant difference between the two groups (p<0.05). CONCLUSION: We found that aloe vera has an anti-irritant effect on the stinging sensation caused by lactic acid.
Subject(s)
Aloe , Bites and Stings , Cheek , Cosmetics , Dermatitis, Atopic , Eczema , Immunomodulation , Lactic Acid , Plants, Medicinal , Sensation , Soaps , Suntan , Wound HealingABSTRACT
BACKGROUND: Aloe vera has been used for medical purposes since antiquity, so it is known as the healing plant or silent healer. It has been claimed that aloe has several important therapeutic properties, including wound healing, thermal injury healing and anti-inflammation and immunomodulation properties. Because of these effects, it has become an ingredient in a wide variety of cosmetic products, including night creams, soaps, shampoos, suntan lotions and cleansers. OBJECTIVE: In order to determine the anti-irritant effect of aloe vera, we compared the degree of stinging intensity caused by 10% lactic acid solution and a mixture of 20% lactic acid and 100% aloe vera. METHODS: We carried out sting tests on 34 volunteers who were all over 20 years of age and they had no past and present history of atopic dermatitis and other eczema. The lactic acid sting test consisted of a 10-minute exposure on the left cheek with 10% lactic acid solution using a Hilltop chamber and the subjects recorded the degree of stinging intensity by themselves every 1 minute during 10-minutes of exposure. We also carried out a sting test with a mixed solution of 20% lactic acid and 100% aloe vera, and we compared the intensity and onset time of the stinging sensation. RESULTS: Aloe vera had some effect to ameliorate the intensity of the stinging sensation and also to delay the time required for the stinging sensation caused by lactic acid. There was a significant difference between the two groups (p<0.05). CONCLUSION: We found that aloe vera has an anti-irritant effect on the stinging sensation caused by lactic acid.
Subject(s)
Aloe , Bites and Stings , Cheek , Cosmetics , Dermatitis, Atopic , Eczema , Immunomodulation , Lactic Acid , Plants, Medicinal , Sensation , Soaps , Suntan , Wound HealingABSTRACT
BACKGROUND: Aloe vera has been used for medical purposes since antiquity, so it has been known as the healing plant or silent healer. It has been claimed that aloe has several important therapeutic properties including promotion of wound healing, thermal injury healing, anti-inflammation and immunomodulation. Using these effects, it has become an ingredient in a wide variety of cosmetic products including night creams, soaps, shampoos, suntan lotions, and cleansers. OBJECT: In order to investigate the anti-irritant effect of aloe vera we measured transepidermal water loss (TEWL) and erythema index (E-index) after performing a patch test using sodium lauryl sulfate (SLS) and aloe vera we then compared the extent of damaging effect on the skin barriers and the difference of the recovery process in each case. METHODS: Fifteen volunteers, all over 18 years of age, who hadn't had past or present history of atopic dermatitis and other eczemas, were tested in this study. SLS was dissolved in distilled water to the concentration of 1% and aloe vera gel was dissolved in distilled water to the concentrations of 10, 20, 50, and 100%. Then we mixed 1% SLS solution and each concentration of aloe vera solution with the ratio 1: 1. Each solution was applied to marked sites of both volar aspect of the forearm at random, for 24 hours, using large Finn chamber with filter paper. TEWL and E-index were measured before test (BL) and at 30 minutes (D0), 1st day (D1), 2nd day (D2), 1st week (W1), 2nd week (W2), and 3rd week (W3) after removal of the patches. RESULTS: TEWL after removing a patch of 0.5% SLS was higher than that of 100% aloe vera mixed with 1% SLS. In addition, TEWL after removing a patch of 0.5% SLS and 100% aloe vera patch mixed with 1% SLS were higher than that of 100% aloe vera. E-index after removing a patch of 0.5% SLS was higher than that of 50% aloe vera mixed with 1% SLS, 100% aloe vera mixed with 1% SLS, and 100% aloe vera. CONCLUSION: We have come to know that aloe vera has an effect in the recovery of skin barriers and erythema induced by irritant such as SLS. However, it only happens when the gel of aloe vera has a high concentration.