Selective COX-2 Inhibitor (Meloxicam) and Tooth-Supporting Bone Quality. A Histomorphometric Study in Rats

Abstract The effects of the non-steroidal anti-inflammatory drugs (NSAIDs) on bone quantity and quality were investigated for years. However, there is lack of information on the impact of NSAIDs on the quality of tooth-supporting alveolar bone in absence of periodontal inflammation. Thus, the aim of this study was to evaluate histometrically the influence of a selective COX-2 NSAID (Meloxicam) on the inter-radicular bone mineral density in rats. Forty-nine adult male Wistar rats were randomly divided into four experimental groups: Subcutaneous injection of 0.9% sterile saline for 15 days (G1; n=12) and 45 days (G2; n=11); and subcutaneous injection of Meloxicam for 15 days (G3; n=13) and 45 days (G4; n=13). Mineral density was histometrically determined in the inter-radicular area of the 1st mandibular molars and data analysis performed by two-way ANOVA (a=5%). Results showed no interaction between time and treatment (p>0.05) and that meloxicam did not affect the alveolar bone density. In contrast, it was found that inter-radicular alveolar bone density increased with time (91.88±3.08% and 92.86±2.38% for groups 15 and 45 days, respectively) (p<0.05). Within the limits of this study, daily administration of a selective COX-2 inhibitor (Meloxicam) did not affect the quality of the inter-radicular alveolar bone in absence of periodontal infection.

Resumo Os efeitos dos fármacos anti-inflamatórios não esteroidais (AINEs) sobre a quantidade e qualidade óssea tem sido investigados ao longo dos anos.Entretanto, há falta de informação sobre o impacto dos AINEs na qualidade do osso alveolar de suporte na ausência de inflamação periodontal. Assim, o objetivo deste estudo foi avaliar, histometricamente, a influência de um AINE seletivo para COX-2 (Meloxicam) na densidade mineral óssea inter-radicular em ratos. Quarenta e nove ratos Wistar, machos e adultos foram divididos aleatoriamente em quatro grupos experimentais: injeções subcutâneas de 0,9% de solução salina estéril por 15 dias (G1, n=12) e 45 dias (G2, n=11); e injeções subcutâneas de Meloxicam por 15 (G3, n=13) e 45 dias (G4, n=13). A densidade mineral foi determinada histometricamente na área inter-radicular dos primeiros molares mandibulares e a análise dos dados realizada por meio de ANOVA (a=5%). Os resultados mostraram nenhuma interação entre tempo e tratamento (p>0,05) e que o meloxicam não afetou a densidade óssea alveolar. Em contraste, foi encontrado que a densidade óssea alveolar inter-radicular aumentou ao longo do tempo (91,88±3,08% e 92,86±2,38% para os grupos 15 e 45 dias, respectivamente) (p<0,05). Dentro dos limites deste estudo, a administração diária de um inibidor seletivo para COX-2 (Meloxicam) não afetou a qualidade do osso alveolar inter-radicular na ausência de infecção periodontal.

Effect of orthodontic treatment on alveolar bone density and epidermal growth factor expression in patients with periodontal disease / 口腔疾病防治

Objective@#To study the effect of orthodontic treatment on alveolar bone density and epidermal growth factor (EGF) expression in patients with periodontal disease.@*Methods @# 50 patients with periodontal disease admitted into our hospital from January 2015 to January 2016 were selected as research object. @*Results @# Before treatment, patients were given dental health instruction. Under the conditions of patients agreeing and cooperating, patients received orthodontic treatment. The changes of alveolar bone density, EGF in blood, saliva, gingival crevicular fluid (GCF), indexes of periodontal inflammation (gingival index, papilla Bleeding index, probing depth) of patients before and after treatment for one month were compared. Before treatment, the mandibular alveolar bone density was significantly higher than maxillary alveolar bone density (P < 0.05); After treatment, the maxillary and mandibular alveolar bone density were significantly lower than before treatment, and maxillary bone density was significantly lower than mandibular bone density (P < 0.05); After one-month treatment, the concentration of EGF expression in blood, saliva compared with before treatment, there was no statistical significance (P > 0.05); After one-month treatment, the concentration of EGF expression in GCF was significantly higher than before treatment (P < 0.05); Before and after treatment for one month, the concentration of EGF expression in GCF was significantly higher than blood, saliva (P < 0.05); After treatment, the GI, PBI, PD levels were significantly lower than before treatment (P < 0.05); After treatment, flow of saliva and GCF were significantly higher than before treatment (P < 0.05).@*Conclusion@#Orthodontic treatment has certain influence on EGF expression in GCF, but has no obvious influence on EGF expression in blood and saliva; The increase of EGF expression derives from periodontal tissue cells and has close association with periodontal remodeling, periodontal tissue cells can promote self-remodeling through autogenous regulation and local humoral regulation.

Effects of Nonsurgical Periodontal Treatment on the Alveolar Bone Density

The aim of this study was to evaluate the effects of nonsurgical periodontal treatment on alveolar bone density (ABD) and bone height (BH) using direct digital radiography. Nineteen patients (mean age: 36±7.3 years) with generalized chronic periodontitis were examined at baseline, 90 (90AT) and 180 (180AT) days after nonsurgical periodontal therapy. Radiographs were taken from two sites with specific characteristics: 39 sites with probing pocket depth (PPD)≤3 mm and clinical attachment level (CAL)≤1 mm (shallow sites); and 62 sites with PPD≥5 mm and CAL≥3 mm (deep sites). The ABD was assessed considering the bone regions of interest at the alveolar bone crest (ROI I) and at the medullar bone (ROI II). The BH was assessed considering the distance from the alveolar bone crest to the cementoenamel junction. Mann-Whitney test was used for the overall demographic data, Wilcoxon test was used to compare the baseline, 90AT and 180AT data as well as to compare the groups and subgroups within the same evaluation period. The significance level was set at 5%. The deep sites showed a significant increase of ABD in ROI I at 90AT (p<0.007) and at 180AT (p<0.005). ABD decrease was seen in ROI II at 180AT (p<0.04) while BH reduced only in shallow sites at 90AT. In conclusion, an increase in ABD was observed in deep sites of patients with generalized chronic periodontitis. However, no significant change in alveolar BH was observed in these sites.

O objetivo desse estudo foi avaliar os efeitos do tratamento periodontal não cirúrgico na densidade do osso alveolar e na altura óssea alveolar usando radiografias digitais diretas. Dezenove pacientes (média de idade 36±7,3 anos) com pacientes com periodontite crônica generalizada foram examinados no tempo 0 e aos 90 (90AT) e 180 (180AT) dias após o tratamento periodontal não cirúrgico. Dois grupos de sítios foram radiografados, 39 com profundidade de bolsa a sondagem (PBS)≤3 mm e nível de inserção clínica (NIC)≤1 mm (sítios rasos) e 62 com PBS≥5 mm and NIC≥3 mm (sítios profundos). A densidade foi avaliada considerando as regiões ósseas de interesse na crista óssea alveolar (ROI I) e no osso medular (ROI II). A altura óssea compreendia a distância entre a crista óssea alveolar e a junção cemento-esmalte. Os sítios profundos mostraram um significante aumento na densidade óssea na ROI I tendo p<0,007 em 90AT e p<0,005 em 180AT. Uma redução na densidade óssea foi vista na ROI II 180AT (p<0,04) enquanto a altura óssea reduziu somente nos sítios rasos 90 AT. Como conclusão, um aumento na densidade foi observado na crista óssea alveolar de sítios profundos em pacientes com periodontite. No entanto, nenhuma alteração significante na altura óssea alveolar foi observada nesses sítios.

Evaluation of alveolar bone density by intraoral periapical radiography / 대한치과보철학회지

PURPOSE: A method detecting change of jaw or alveolar bone density may be helpful in periodontal care, implant dentistry and evaluation of bone density of whole body. MATERIALS AND METHODS: In this study, bone density of intraoral periapical radiography using phantom-integrated XCP is compared with that of quantitative computed tomography (QCT). RESULTS: Bone density of intraoral periapical radiography and the one measured by QCT showed high correlation (correlation coefficient = 0.92, P<.001) in alveolar bone, and relatively high correlation (0.73, P<.001) in cancellous bone. CONCLUSION: This study revealed possibility of scoring of bone density by intraoral periapical radiography.

Radiological analysis of the relationship between occlusal tooth wear and mandibular alveolar bone density and height.

Aim: The aim of this study was to examine the relationship between occlusal tooth wear and mandibular alveolar bone density (ABD) and alveolar bone height (ABH) using computer-assisted densitometric image analysis (CADIA) program. Materials and Methods: The study was comprised of 134 mandibular first molar teeth in 90 patients with various degrees of occlusal tooth wear. The degree of tooth wear was classified according to the tooth wear index (TWI). Periodontal examinations were performed on all patients and included plaque index, gingival index, probing depth, and clinical attachment loss measurements. ABD and ABH were measured from intraoral digital periapical radiographs and analyzed using CADIA. Results: No increases in ABD were observed between TWI 0 and TWI 1 or between TWI 0 and TWI 2 (P > 0.05). However, increases in ABD were observed between TWI 0 and TWI 3 (P < 0.01), TWI 1 and TWI 3 (P < 0.01), TWI 1 and TWI 2 (P < 0.05) and TWI 2 and TWI 3 (P < 0.05). In addition, ABH was found to increase with increase in wear, with the exception of the interval between TWI 0 and TWI 1. Conclusions: Occlusal tooth wear does not result in any radiologically identifiable changes in the alveolar bone during the initial stages of wear; however, significant increases in both ABD and ABH may be observed as tooth wear progresses.

Related factors and effectiveness of rapid teeth movement through reducing resistance and distraction / 中南大学学报(医学版)

Objective:To investigate the role of reducing resistance and distraction in rapid teeth movement and its reliability by establishing the Beagle dogs’ experimental model. Methods:The left or right sides in mandibles of 20 beagles were randomly operated with different treatments:distraction twice a day through reducing resistance;distraction 6 times a day through reducing resistance;conventional distraction through reducing resistance;and conventional distraction (the control group). Each treatment was carried out in 10 sides. The pulp vitality, tooth mobility and distance of teeth transportation were evaluated at different time points:before the distraction, distraction after 15 days, retaining 30 days after 15 days of distraction. The degree of inclination, root resorption and alveolar bone density of the compressive areas were evaluated by cone-beam computed tomography images. Results:The distance of teeth transportation was similar in groups distraction twice daily and 6 times a day through reducing resistance (P>0.05), but their speed of transportation was significantly higher than that of conventional distraction through reducing resistance. The conventional distraction group had the lowest speed of transportation. The pulp vitality of distracted teeth was normal, and no root comprehensive resorption and periodontal defect were found. Distracted teeth in the reduced resistance and distraction groups (13.9°±3.5°) tipped more that in the conventional distraction group (6.6°±1.3°) (P Conclusion:Reducing resistance and distraction are inseparable factors to realize fast teeth moving. The rate of orthodontic tooth movement can be accelerated through resistance reduction and periodontal distraction without obvious unfavorable effects but at minimal acceptable teeth inclination.

Efeitos do tratamento periodontal não cirúrgico sobre a densidade e altura óssea alveolar / Effects of non surgical periodontal treatment on the alveolar bone dentity

O objetivo deste trabalho foi avaliar o efeito do tratamento periodontal não cirúrgico (TPNC), na densidade e na altura óssea alveolar, em pacientes com periodontite, utilizando radiografias digitais diretas. Cento e um sítios, em dezenove pacientes (idade média 36 ± 7.3 anos) foram acompanhados no dia 0, e 90 e 180 dias após TPNC. Os índices clínicos de profundidade de bolsa a sondagem (PBS), nível de inserção clínica, sangramento à sondagem e índice de placa foram registrados e radiografias digitais foram feitas. A densidade foi analisada através de regiões ósseas de interesse colocadas sobre a crista óssea alveolar (ROI I) e sobre o osso medular (ROI II). A altura óssea alveolar foi medida através da distância da crista óssea alveolar até a junção cemento esmalte. Os sítios profundos (PBS ≥ 5mm) apresentaram uma melhora clínica significante (p <0.01), acompanhada de um aumento na densidade da ROI I (p <0.01). A ROI II mostrou um aumento na densidade dos sítios com PBS ≤ 3mm em pacientes com periodontite agressiva (p <0.05). No entanto, houve diminuição nos sítios com PBS ≥ 5mm nesses mesmos pacientes (p <0.03). A altura óssea alveolar não sofreu alteração após TPNC. Após o tratamento periodontal não cirúrgico, observou-se que as radiografias obtidas através da técnica digital direta parecem mostrar um aumento na densidade da crista óssea, nos sítios profundos dos pacientes com periodontite. No entanto, a redução da profundidade de bolsa e do ganho no nível de inserção clínica não foi acompanhada por alterações significantes na altura óssea alveolar nestes sítios.

The aim of this study was to access the effects of non-surgical periodontal treatment (NSPT), on the bone density and alveolar bone height (ABH), in patients with periodontitis, using direct digital radiographs. One hundred one sites in nineteen patients (mean age 36 ± 7.3 years) were accompanied on day 0, and 90 and 180 days after the TPNC. The clinical scores of probing pocket depth (PPD), clinical attachment level, bleeding on probing and plaque index were registered and digital radiographs were taken. The density was accessed considering the bone regions of interest at the alveolar bone crest (ROI I) and the medullar bone (ROI II). The ABH measured the distance between the alveolar bone crest and the cementoenamel junction. The deep sites (PPD ≥ 5mm) presented a clinical improvement (p <0.01), accompanied by an increase in bone density at ROI I (p <0.01). ROI II showed an increase in density at sites with PPD ≤ 3mm in patients with aggressive periodontitis (p <0.05). However, there was a decrease in density in sites with PPD ≥ 5mm of the same patients (p <0.03). The ABH didn´t change after TPNC. After non-surgical periodontal treatment, the direct digital radiographs showed a significant increase in bone crest density of deep sites in patients with periodontitis. Moreover, the reduction in the probing pocket depth and the increase in the attachment level were not followed by changes in alveolar bone height in these sites.

Efeitos do tratamento periodontal não cirúrgico sobre a densidade e altura óssea alveolar / Effects of non surgical periodontal treatment on the alveolar bone dentity

O objetivo deste trabalho foi avaliar o efeito do tratamento periodontal não cirúrgico (TPNC), na densidade e na altura óssea alveolar, em pacientes com periodontite, utilizando radiografias digitais diretas. Cento e um sítios, em dezenove pacientes (idade média 36 ± 7.3 anos) foram acompanhados no dia 0, e 90 e 180 dias após TPNC. Os índices clínicos de profundidade de bolsa a sondagem (PBS), nível de inserção clínica, sangramento à sondagem e índice de placa foram registrados e radiografias digitais foram feitas. A densidade foi analisada através de regiões ósseas de interesse colocadas sobre a crista óssea alveolar (ROI I) e sobre o osso medular (ROI II). A altura óssea alveolar foi medida através da distância da crista óssea alveolar até a junção cemento esmalte. Os sítios profundos (PBS ≥ 5mm) apresentaram uma melhora clínica significante (p <0.01), acompanhada de um aumento na densidade da ROI I (p <0.01). A ROI II mostrou um aumento na densidade dos sítios com PBS ≤ 3mm em pacientes com periodontite agressiva (p <0.05). No entanto, houve diminuição nos sítios com PBS ≥ 5mm nesses mesmos pacientes (p <0.03). A altura óssea alveolar não sofreu alteração após TPNC. Após o tratamento periodontal não cirúrgico, observou-se que as radiografias obtidas através da técnica digital direta parecem mostrar um aumento na densidade da crista óssea, nos sítios profundos dos pacientes com periodontite. No entanto, a redução da profundidade de bolsa e do ganho no nível de inserção clínica não foi acompanhada por alterações significantes na altura óssea alveolar nestes sítios.

The aim of this study was to access the effects of non-surgical periodontal treatment (NSPT), on the bone density and alveolar bone height (ABH), in patients with periodontitis, using direct digital radiographs. One hundred one sites in nineteen patients (mean age 36 ± 7.3 years) were accompanied on day 0, and 90 and 180 days after the TPNC. The clinical scores of probing pocket depth (PPD), clinical attachment level, bleeding on probing and plaque index were registered and digital radiographs were taken. The density was accessed considering the bone regions of interest at the alveolar bone crest (ROI I) and the medullar bone (ROI II). The ABH measured the distance between the alveolar bone crest and the cementoenamel junction. The deep sites (PPD ≥ 5mm) presented a clinical improvement (p <0.01), accompanied by an increase in bone density at ROI I (p <0.01). ROI II showed an increase in density at sites with PPD ≤ 3mm in patients with aggressive periodontitis (p <0.05). However, there was a decrease in density in sites with PPD ≥ 5mm of the same patients (p <0.03). The ABH didn´t change after TPNC. After non-surgical periodontal treatment, the direct digital radiographs showed a significant increase in bone crest density of deep sites in patients with periodontitis. Moreover, the reduction in the probing pocket depth and the increase in the attachment level were not followed by changes in alveolar bone height in these sites.

The bone density of mandible as the aging process in Koreans

INTRODUCTION: This study compared the alveolar bone density of the mandible according to gender, age and position using Cone-beam computed tomography (CT). MATERIALS AND METHODS: The maxillofacial CT scan data was obtained from 60 Korean patients. In addition, the alveloar bone density of 5 males and 5 females with normal occlusion aged from 10 to 70 years was measured at the buccal cortical bone, cancellous bone and lingual cortical bone, as well as at the position of the incisors, canines, premolars and molars. RESULTS: The age-specific mean bone density was highest in patients in their third decade. The buccal cortical bone of the molars showed the highest bone density. Males in their fifties and sixties had a higher bone density in the cancellous bone in the region of the premolars and the buccal cortical bone of the molars, respectively, than females but there was no significant difference between males and females in the other parts. The cancellous bone density was highest in those in their twenties and thirties, and tended to decline up to their seventh decade. CONCLUSION: These results revealed a significantly different bone density according to gender, age and position in the Korean population. In addition, it is possible to predict the bone density based on these results.