Study of diagnose guinea pigs anaphylaxis shock with RBL-2H3 cells / 中国免疫学杂志

Objective:In order to study of diagnose anaphylactic shock in forensic field,a new in vitro testing sysytem were established.Methods:The RBL 2H3 were cultured and sensitized,the release of histamine and stained for Annexin V were testing by fluorescent spectrometer and flow cytometry respectively.Results:The degree of RBL 2H3 degranulation after sensitived serum treated was higher than that after control serum treated,and the degranulation degree increased dose dependently.Conclusion:RBL 2H3 are helpful to diagnose anaphylactic shock,and the release of histamine and the positive staining for Annexin V may be the ideal indices.