ABSTRACT
Objective: DNA molecular identification of medicinal plants in Angelica L. was carried out based on the internal transcribed spacer (ITS) of ribosomesequence variation. Methods: One hundred and fifty-eight individuals of 32 species from Angelica L. were selected, and ITS sequences were used to evaluate the differences among these species. Obtained sequences were analyzed using MEGA6.0 program with the kimura-2-parameter (K2P) model. Meanwhile, phylogenetic trees were constructed, included the neighbor-joining (NJ) tree, the maximum-likelihood (ML) tree, and the maximum-parsimony (MP) tree, and we determined the identification ability of ITS sequences and the phylogenetic relationships among the species in Angelica L. Results: The minimum interspecific distance is far greater than the maximum intraspecific distance in ITS region. The phylogenetic analysis showed that the species in Angelica L. can be easily differentiated according to their strong bootstrap support and relationships. Conclusion: ITS region as DNA barcode can distinguish the medicinal plants in Angelica L. and would be a foundation for the aid of molecular identification of the species in Angelica L.
ABSTRACT
Objective To establish the base of molecular system-theory on medicinal plants of Angelica L.and to offer molecular gists for finding the efficacy difference of them.Methods The PCR technique was utilized together with the clone technique to get the ITS gene and measure its genic sequence.Statistical analyzing and branch analyzing were carried out by Phylip software and genetic distance difference in every kinds can be calculated with MEGA3.Results ITS1-5.8 S-ITS2,belongs to one of medicinal plants of Angelica L.,its sequence length extents 600 to 629 bp.Subtracting 5.8 S,its length is 165 bp,the length of ITS is 468 bp.The medicinal plants of Angelica L.are divided into two groups by dendrogram.The genetic distance of A.sinensis from Gansu Province is obviously different from the other medicinal plants of Angelica L.,but the distance difference compared with Levisicum officinale is smaller.Conclusion It suggests that the above result be identical to the efficacy of medicinal plants of Angelica L.It may be an important reason of explaining why the efficacy between A.sinensis and other medicinal plants of Angelica L.is so different.