ABSTRACT
Aim To investigate whether low dose gem- citabine ( GEM) could enhance the anti-pancreatic cancer effects of human umbilical vein endothelial cell (HUVEC) vaccine.Methods C57BL/6 mice were randomly divided into four groups; PBS control group, GEM group, HUVEC vaccine group and HUVEC vaccine combined GEM group ( HUVEC-GEM group).Mice were inoculated with Pan02 pancreatic cancer cells to establish a subcutaneous xenograft model to observe tumor growth and adverse reactions in tumor bearing mice.Whether GEM could enhance the immune responses induced by HUVEC vaccine was determined by ELISA analysis of the immune serum, splenic lymphocyte proliferation assay, cytotoxic T lymphocyte killing assay and IFN-7 content assay.Results The results of subcutaneous transplantation tumor model showed that the introduction of GEM into the HU-VEC vaccine treatment could enhance the therapeutic anti-pancreatic cancer effects of HUVEC vaccine.Enzyme-linked immunoassay results confirmed that GEM acted as an immune adjuvant could effectively increase the HUVEC antibodies and I FN-7 level in the immune serum of mice.The results of lymphocyte proliferation experiment and CTL killing activity assay indicated that GEM could effectively enhance the spleen lymphocyte transformation activity and CTL killing ability of HUVEC vaccine immunized mice.Conclusions Low dose GEM could enhance the immune responses induced by HUVEC vaccine and thus enhance the anti- Pan02 pancreatic cancer effects of HUVEC vaccine.
ABSTRACT
Objective To evaluate the diagnostic value of dynamic contrast enhanced MRI (DCE-MRI)in angiogenesis and invasion of ovarian tumors .Methods 98 patients with pathological-confirmed ovarian tumors without any treatment underwent plain MRI and DCE-MRI before operation .The plain MR images and DCE-MRI data were retrospectively analyzed .Value of Ktrans and Ve was detected by pharmacokinetic model .The expression of VEGF and MMP-2 in ovarian tumors was examined by immunohistochemistry .The correlation between DCE-MRI parameters and immunohistochemical results was analyzed .Results The expression of VEGF and MMP-2 was sig-nificantly higher in the malignant group compared with that in the benign group (both P<0.05).The value of Ktrans and Ve was significantly lower in the benign group compared with that in the malignant group ( both P<0.05) .The value of Ktrans and Ve was positively correlated with the expression of VEGF and MMP-2.Conclusion DCE-MRI can noninvasively evaluate the angiogenesis and invasion of ovarian tumors in vivo , which has important signifi-cance for clinical treatment and prognostic assessment .
ABSTRACT
Objective To discuss the angiogenic e ffects on tumor micrometastasis. Methods Literatures on the relation between tumor angiogenesis and micrometastasis were reviewed. Results Tumor angiogenesis was a basis of the devel opment of micrometastasis.Conclusion Micrometastic dependence on angiogenesi s gestates a novel revolution of tumor treatment.