ABSTRACT
ObjectiveTo evaluate the effect of losartan on ventricular arrhythmia in elderly patients with hypertension.Methods The 78 hypertension subjects with ventricular arrhythmia aged 65 to 89 years were randomly assigned to treatment with losartan or placebo (enalapril) for 12 months. The blood pressure, left ventricular mass index and ventricular arrhythmia were observed and analyzed.ResultsAfter 12 months treatment, the pressure lowering effect was similar in losartan versus enalapril groups, there were no differences in systolic pressure decrement and diastolic pressure decrement between the two groups (P＞0. 05). Left ventricular mass index was lower in enalapril groupthaninlosartangroup [(109.2±15. 4) g · m-2 vs. (128.5±16. 7) g · m-2, t=2.015, P＜0. 05].However, the prevalence of ventricular arrhythmia was lower in losartan group than in enalapril group [(628. 5±176.8)/24 h vs. (852.9±215.7)/24 h, t=2.417,P＜0.05]. No Pearson's correlations of reduction of ventricular arrhythmia with reduction of blood pressure (systolic: r=0. 094, P＞0. 05; diastolic: r= 0.08, P＞0. 05) and reduction of left ventricular mass index were found. in losartan group.Conclusions Initiation of antihypertensive treatment with losartan in elderly subjects appears to cause more reduction of ventricular arrhythmia than with enalapril, despite similar reduction of blood pressure.
ABSTRACT
Objective To investigate the effects and mechanisms of selective angiotensinⅡtype 1 receptor antagonist ZD7155 on the inhibition of pancreatic cancer in vitro.Methods MTT assays were used to determine the inhibition of pancreatic cancer cell line PaTu8988s by ZD7155 in different concen- trations and at different time.PaTu8988s cell cycle and cell apoptosis were detected by flow cytometry. Transmission electron microscope was used to investigate the apoptosis of PaTu8988s before and after the incubation with ZD7155 under different concentrations.PaTu8988s cell morphology was observed be- fore and after the incubation with ZD7155.Results MTT showed that the increase of inhibition of pan- creatic cancer cell by ZD7155 was in agreement with the increase of the concentrations of ZD7155 and the time of the incubation with ZD7155.The inhibition rates of PaTu8988s cells were 9%,18%,30%, 51%,60% and 78% by ZD7155 with the concentrations of 5?10~(-11),5?10~(-10),5?10~(-9),5?10~(-8),5?10~(-7) and 5?10~(-6) mol/L,respectively.The inhibition rates of PaTu8988s cells were 15%,25%, 36%,51%,67% and 85% by ZD7155 with the same concentration(5.0?10~(-8) mol/L)at 12,24,36, 48,60 and 72 hours,respectively.ZD7155 could also inhibit PaTu8988s cell cycle significantly and was dose-dependent.Cell electron microscopy showed that there were chromatin margination and apoptotic body in the cell nucleus when the cells were incubated with ZD7155,and these changes were increase with the concentrations of ZD7155.The morphology of PaTu8988s cell didn't have any change after in- cubation with ZD7155.Conclusions ZD7155 can inhibit the growth of pancreatic cancer cells in vitro by suppressing the S-phase of cell cycle and induce cell apoptosis without visible cell toxic effects.