ABSTRACT
Aim To establish human U87-MG glioma model in nude mice brain and to observe the characteristics of the tumor growth. Methods Human U87-MG glioma cells were cultured in vitro. 5 μL of cell suspension containing 3.0 ×1010·L-1, 4.0×1010·L-1and 5.0×1010·L-1respectively was inocula-ted into the right caudate nucleus of 18 male nude mice brain un-der the guidance of stereotaxic apparatus, separately, whereas another 6 nude mice as the control group, were inoculated into the same volume of Hanks solution. The moving and survival state of rats with gliomas were observed. The examinations of the tumors formation, volumes, metastasis and histopathology were performed and the obtained brain samples were stained with HE and immunohistochemistry. Results All the tested rats of dif-ferent inoculation doses developed brain tumors without extracra-nial metastasis. The mean survival time of three groups was (46.50 ± 3.27) d,(38.50 ± 3.28) d and (30.67 ± 3.51) d,respectively. The tumors showed the similar morphological fea-tures and immunophenotype to human glioma. There was positive expression of GFAP and S-100 in the tumors. Conclusions The orthotopic implantation model of human U87-MG glioma, by in-oculating quantitative U87-MG cells stereotaxically into the brains of the nude mice, is successfully established with 100 yield of intracranial tumor and no extracranial growth extension. It resembles the histopathological and morphological features of human glioma,which can be used as a reliable animal model for the study of the tumorigenesis, pathogenesis, biological charac-teristics and therapy of glioma.
ABSTRACT
Objective To evaluate the effectiveness of a major depression animal model for old man es?tablished with orchidectomized(ORX) and subsequently olfactory bulbectomized(OBX) rat. Methods Healthy Sprague?Dawley male adult rats were randomly assigned to six groups with ten rats in each group:intact,sham?oper?ated,model,vehicle,fluoxetine plus testosterone propionate(Flu+TP) and amitriptyline plus testosterone propionate (Ami+TP) groups. ORX+OBX rat model was established. Therapy of Flu or Ami (10 mg/kg,i.p.) combined with TP(25 mg/kg,s.c.) was administered. Behavioral changes of rats in each group were detected. Results Open field test: The number of rat crossing lattices in model((124.50±14.25)lattices) or vehicle group( 118.70± 10.27) was significantly larger than those of intact(38.20±6.30),sham?operated(33.70±9.58),Flu+TP (34.00± 9.82) and Ami+TP group (35.20±12.54). The rearing number of model or vehicle group was also significantly larger than those of intact,sham?operated,Flu+TP and Ami+TP group.Statistical differences in the above corre?sponding comparisons of open field test existed (P<0.01). Forced swimming test: The immobility time of model ((131.70±32.44)s) or vehicle group ((135.10±34.01)s) was significantly longer than those of intact((66.60± 12.06)s),sham?operated((62.00±13.38)s),Flu+TP((59.60±8.89)s) and Ami+TP group ((64.80±10.78)s). There were statistical differences in the above corresponding comparisons (P<0.01). Morris water maze test: The escape latency of model((62.00±26.05)s) or vehicle group ((60.52±27.00)s) was significantly longer than those of intact((24.19±9.12)s),sham?operated((21.06±8.13)s),Flu+TP((22.88±8.01)s) and Ami+TP group ((27.04±10.11)s);while the time rat swimming in the quadrant originally put a platform of model or vehicle group was significantly shorter than those of intact,sham?operated,Flu+TP and Ami+TP group. There were statisti?cal differences in the above corresponding comparisons of Morris water maze test (P<0. 01, P<0. 05 ) . Conclusion The above data demonstrates that ORX+OBX rat model is both repeatability and reliability,and can be a major depression animal model of old man for future researches.