ABSTRACT
Descrevem-se o quadro clínico, patológico e o potencial tóxico das folhas verdes de Crotalaria pallida em ovinos. Para o experimento folhas verdes de C. pallida foram coletadas no município de São João do Sul em Santa Catarina e administradas por via oral para seis ovinos adultos, em doses únicas de 40, 20, 10, 5, 2,5 e 2,5g/kg, e para dois ovinos, em doses diárias de 0,63 e 1,25g/kg durante 30 dias. Reproduziu-se a intoxicação com doses letais a partir de 2,5g/kg. Um dos ovinos que recebeu a dose única de 2,5g/kg e os que receberam as doses diárias, não adoeceram. Todos os ovinos que apresentaram sinais clínicos morreram entre 13 e 22 horas após a administração das folhas. Sinais clínicos leves como fezes pastosas, inquietação e aumento das frequências cardíaca e respiratória tiveram início cerca em 11 horas após a ingestão da planta. Minutos antes da morte observaram-se dispneia intensa com respiração abdominal pronunciada. As principais lesões macroscópicas foram restritas à cavidade torácica, como hidrotórax e edema pulmonar acentuado. O exame microscópico revelou no pulmão, edema acentuado e difuso na superfície pleural, nos espaços interlobulares e ao redor das artérias peribronquiais e peribronquiolares. Edema multifocal foi observado no interior de alvéolos e ao redor de brônquios e bronquíolos, além de leve infiltrado neutrofílico multifocal. O quadro clínico-patológico da intoxicação experimental foi similar ao observado na intoxicação espontânea e caracterizou-se por edema iulmonar acentuado e morte logo após o início dos sinais clínicos.(AU)
This paper describes the toxic potential of the green leaves of Crotalaria pallida and the clinical signs in experimentally poisoned sheep. Green leaves of C. pallida were collected in São João do Sul, Santa Catarina, and administered orally to six adult sheep, as single dose of 40, 20, 10, 5, 2.5 and 2.5g/kg, and for two sheep at doses of 0.63 and 1.25g/kg during 30 days. The poisoning was reproduced at lethal doses of 2.5g/kg. One of the sheep that received a single dose of 2.5g/kg and another that received daily doses did become ill. All sheep wich developed clinical signs died between 13 and 22 hours after administration of the plant. Slight signs started in about 11 hours after ingestion of the plant, and clinical signs included loose stool, restlessness and increased heart and respiratory rates. A few minutes preceding death, pronounced abdominal breathing and severe dyspnea was observed. The main gross lesions were restricted to the thoracic cavity, as severe pulmonary edema and hydrothorax. Lung microscopy showed marked edema in the diffuse pleural surface, the interlobular spaces and around the peribronchial arteries. Multifocal edema was observed within alveoli and around the bronchi and bronchioles, and mild multifocal infiltration of neutrophils. The clinical and pathological alerations of the experimental poisoning was similar to that observed in the spontaneous poisoning, characterized by marked pulmonary edema and death shortly after the onset of clinical signs.(AU)
Subject(s)
Animals , Crotalaria/toxicity , Dyspnea/veterinary , Plant Poisoning/veterinary , Pulmonary Edema/veterinary , Sheep , Plants, ToxicABSTRACT
Aims: This study focused on the serologic detection of Toxoplasma gondii infection in two groups of cats:stray and household groups. In addition, hematologic assessment of seropositive and seronegative cats was done. Methods: Sixty cats were serologically tested for anti-Toxoplasma gondii antibodies using the latex agglutination test. Six collection sites for each group of cats were identified in the urban communities of Sta Rosa and San Pedro, Laguna, Philippines. The 60 cats collected were divided into 30 stray and 30 household cats. Results: Results revealed that 28 (46.67%) of the 60 cats were seropositive. There were more household cats (28.33%) which showed seropositivity compared to stray cats (18.33%), however the difference was statistically insignificant (p>0.05) . Hematologic tests through complete blood count showed significantly (p<0.05) higher number of seropositive cats with abnormalities on hemoglobin level, red blood cell count, segmenter (neutrophil) and monocyte counts compared to the control. Other parameters such as percent packed cell volume, white blood cell count, eosinophil and lymphocyte counts showed insignificant (p>0.05) results across seropositive cats and the control. Blood chemistry analysis showed significantly higher (p<0.05) potassium level irregularities in seropositive cats relative to the seronegative cats. Other parameters such as amylase, blood sugar, blood uric acid, creatinine and blood urea nitrogen were statistically insignificant (p>0.05). Conclusions: Although Toxoplasma gondii infection suggests possible cause of hematologic abnormalities, it is recommended that further studies on this aspect be done to provide more basic and clinical research information that would improve cat health management.