ABSTRACT
Objective: Determination of 666 (BHC) and DDT residues in Anoectochili Roxburghii Gemma Terminalis. Methods: Using gas chromatography (GC) for limited detecting the eight kinds of organochlorine pesticide residues, such as BHC (α-BHC, β-BHC, γ-BHC, and δ-BHC) and DDT (PP '-DDE, PP'-DDD, OP '-DDT, and PP'-DDT) in Anoectochili Roxburghii Gemma Terminalis. Results: Linear BHC and DDT standard curve were good, the correlation coefficient was 0.9988-0.9997, the average recovery rates were 95.4%-98.9%, 1%-2.98% RSD. The detection limitation was 0.0053-0.021 g/L. Result of Chinese herbal medici: β-BHC for G 0.0009mg/kg(Among them, α-BHC, γ-BHC, δ-BHC, PP'-DDE, OP'-DDT, PP'-DDD, PP'-DDT not detected) Conclusion: The method is accurate, reliable, scientific, and feasible, and has higher sensitivity and linear range, which can be used for quality control of pesticide residues in water.
ABSTRACT
Objective: To establish the chromatographic fingerprint analysis for the quality of Anoectochili Roxburghii Gemma Terminalis. Methods: The separation was performed on an Agilent TC-C18 column (250 mm × 4.6 mm, 5 μm) with a gradient elution composed of methanol and water. The detective wavelength was at 260 nm. The flow rate was 1.0 mL/min and the column temperature was set at 30℃. Results: Twenty-two characteristic peaks were established in the fingerprint. The mutual model of Anoectochili Roxburghii Gemma Terminalis was established and the similarities were calculated. Seven chromatographic peaks among twenty-two common peaks were identified by LC-MS/MS. Ten batches of Anoectochili Roxburghii Gemma Terminalis were determined and the similarities were above 0.9. Conclusion: The method is accurate with good reproducibility. It can be used for the quality control of Anoectochili Roxburghii Gemma Terminalis.