Molecular approach for ante-mortem diagnosis of rabies in dogs

Background & objectives: The ante-mortem diagnosis of rabies is of great significance in establishing the status of infection in dogs, especially since they are involved in exposure to human beings. The present study was, therefore, undertaken to elucidate the most appropriate secretion/tissue for reliable diagnosis of rabies in 26 living dogs suspected to be rabid. Methods: In the present study 26 dogs suspected to have rabies were included for ante-mortem diagnosis of rabies in clinical samples of skin and saliva by molecular approach viz. heminested reverse-transcriptase polymerase chain reaction (HnRT-PCR). Skin and saliva samples were collected from 13 dogs each. Results: Of the 13 clinically suspected dogs, fluorescent antibody technique (FAT) confirmed rabies in nine cases of dogs. Of these nine true-positive dogs, eight cases could be confirmed by HnRT-PCR from skin. Of the other 13 dogs clinically suspected for rabies, FAT confirmed rabies in 10 cases. Of these 10 true-positive dogs, rabies was detected ante-mortem by HnRT-PCR from the saliva in seven dogs. Thus, rabies was detected from skin with 90 per cent sensitivity, 100 per cent specificity and 92.85 per cent accuracy. With saliva, rabies was detected with a sensitivity of 76.92 per cent, specificity of 100 per cent and accuracy of 62.50 per cent. The positive predictive values were 100 per cent for both skin and saliva samples while negative predictive values were 80 and 50 per cent, respectively. Interpretation & conclusions: Skin biopsy may be more appropriate clinical sample as compared to saliva for ante-mortem diagnosis of rabies in dogs. HnRT-PCR can be employed for molecular diagnosis of rabies from skin in live dogs.

Efecto del tiempo de ayuno sobre el rendimiento en canal y el pH en canales bovinas

Introducción. Los tiempos de espera ante mortem en Colombia son prolongados, el ayuno de los animales supera en promedio las 40 horas, provocando altos niveles de estrés que afectan la calidad y cantidad de la carne. Objetivo. Analizar el efecto del tiempo de espera ante mortem sobre el rendimiento en canal y el pH en canales bovinas. Materiales y métodos. Se estudiaron 78 animales (Brahman comercial) provenientes del municipio de Montería, Córdoba, Colombia; con edades entre 24 y 27 meses, y un peso promedio de 465 kg. Fueron sometidos a dos tiempos de ayuno: uno corto (20 horas) y uno prolongado (40 horas). Se registraron los pesos vivos, de canal caliente y canal fría, y se realizó la medición de pH 24 horas post mortem. Resultados. Se analizó el efecto del tiempo de espera ante mortem sobre el rendimiento en canal y el descenso del pH 24 horas post mortem en canales bovinas; se hallaron diferencias significativas en el descenso del pH 24 horas post mortem, y se observó que la tasa de decrecimiento es casi el doble en los animales con ayunos prolongados y el nivel de estabilización es menor; con respecto al rendimiento en canal no se encontraron diferencias significativas entre los tratamientos. Conclusiones. Se concluye que los tiempos de espera ante mortemprolongados en los bovinos afectan el descenso normal del pH 24 horas post mortem, lo cual tiene repercusión sobre la calidad final de la carne.

Introduction. The ante mortem lapses in Colombia are long, animals do not eat for more than 40 hours in average and this produces high stress levels that affect the quality and the quantity of the meat. Objective. Analyze the effect of the ante mortem waiting time on the performance in carcass and the pH in bovine carcasses. Materials and methods. 78 animals were studied (commercial Brahman) with ages between 24 and 27 months and an average weight of 465 kg. They were subjected to two fasting lapses: a short one (20 hours) and a long one (40 hours). The living weights were registered in hot and cold carcass conditions, and the pH was measured post mortem, after 24 hours. Results. The effect of the ante mortem time of wait on the performance in carcass and the decline of the pH 24 hours post mortem in bovine carcasses were analyzed. Significant differences in the pH decline 24 hours post mortem were found, and it was observed that the decrease rate is almost twice in animals with long fasting and the stabilization level is lower. As for the performance in carcass, there were no significant differences in the treatments. Conclusions. The long ante mortem waiting lapses affect the normal decline of the pH 24 hours post mortem, and this affects the meat´s quality.

Introdução. Os tempos de espera ante mortem na Colômbia são prolongados, o juejum dos animais supera em média as 40 horas, provocando altos níveis de estresse que afetam a qualidade e quantidade da carne. Objetivo. Analisar o efeito do tempo de espera ante mortem sobre o rendimento em canal e o pH em canais bovinas. Materiais e métodos. Se estudaram 78 animais (Brahman comercial) provenientes do município de Montería, Córdoba, Colômbia; com idades entre 24 e 27 meses, e um peso médio de 465 kg. Foram submetidos a dois tempos de jejum: um curto (20 horas) e um prolongado (40 horas). Se registraram os pesos vivos, de canal quente e canal fria, e se realizou a medição de pH 24 horas post mortem. Resultados. Se analisou o efeito do tempo de espera ante mortem sobre o rendimento em canal e o descenso do pH 24 horas post mortem em canais bovinas; se encontraram diferenças significativas no descenso do pH 24 horas post mortem, e se observou que a taxa de decrescimento é quase o dobro nos animais com jejum prolongados e o nível de estabilização é menor; com respeito ao rendimento em canal não se encontraram diferenças significativas entre os tratamentos. Conclusões. Se conclui que os tempos de espera ante mortem prolongados nos bovinos afetam o descenso normal do pH 24 horas post mortem, o qual tem repercussão sobre a qualidade final da carne.

Dental Pathologies in the Three Kingdoms Period Population from Imdang Site, Gyeongsan, Korea / 체질인류학회지

Dental pathology has the potential to provide insight into the interpretation of the health and life style of past population. In particular, dental pathologies are strongly correlated to subsistence pattern, diet habits and food-preparation techniques. For this reason, my studies have focused on analyses of dental diseases (e.g., caries, abscesses, ante-mortem tooth loss, enamel hypoplasia, dental attrition, and periodontal diseases). The purpose of this study is to investigate the prevalence of dental pathologies of the Imdang population dating to between A.D. 3rd and A.D. 7th centuries (Three Kingdoms Period). In this study, through a macroscopic observation of dental remains from 138 individuals of the Imdang site, the prevalence of caries, abscess and ante-mortem tooth loss was examined. The association between sex, age and the frequencies of pathology was analyzed by the Chi-square tests. The results showed that the frequency of dental pathologies was consistently low level in Imdang population. Caries prevalence in the Imdang population was higher in females (6.15%) that in males (4.89). Among dental pathologies, abscess prevalence (0.7%) was lowest in Imdang population. Meanwhile, AMTL prevalence was 10.81% in males and 3.8% in females. There is a statistically significant difference between the anterior teeth of males and females (p<0.05). This study revealed that the rate of dental caries, abscess and AMTL in the Three Kingdoms Period population was relatively low level. This study is the first report of the patterns of dental pathologies in the Three Kingdoms Period population. It is necessary to investigate more number of pathologic markers that needed for deeper understanding of the multi-faceted nature of dental pathologies of ancient populations.

Is necropsy obsolete - An audit of the clinical autopsy over six decades: A study from Indian sub continent.

Introduction: Several studies have documented a decrease in the autopsy rate. This study was taken up to analyse the cause of mortality, the discrepancies between the ante mortem and post mortem diagnosis and the discrepancies between diagnoses according to the type of the disease over a period of six decades. Materials and Methods: Autopsy reports and medical records were retrospectively analyzed over a 63 year period from 1947 to 2010. Results: In our study, there was a steady increase in the percentage of neoplastic cases from 1947 to 1994 after which there has been a signifi cant drop. The cases dying due to infection has also shown a steady decline over the years until 1994. After 1994, there has been a signifi cant increase in the deaths until 2010 (p < 0.05). Death due to cardiac causes has shown an increase until 1962 which has been followed by a steady decline. There has been a sudden rise in the number of cases dying due to renal causes between 1994 and 2000 (p < 0.05). There has been a statistically signifi cant decrease in the discrepancies between the ante mortem and the post mortem diagnosis over the years. Discussion: This study shows that therapeutic and preventive measures correctly instituted have signifi cantly reduce the mortality, particularly with reference to cardiac and infectious causes. The discrepancy between antemortem and post-mortem diagnosis in 2010 is still very high at 9.30 percent. The autopsy will continue to remain relevant especially in elucidating the molecular cause of disease.

Role of reverse transcriptase polymerase chain reaction for the diagnosis of human rabies.

Background & objectives: Traditionally, rabies diagnosis is made by demonstration of rabies viral antigen by direct immunofluorescence (DIF) and mouse inoculation test (MIT). The present study was carried out to evaluate the role of reverse transcriptase-polymerase chain reaction (RT-PCR) in comparison with these conventional techniques for the diagnosis of rabies. Methods: Skin biopsies, corneal impression smears and saliva sample were collected ante-mortem and brain tissue and CSF were collected post-mortem from ten clinically suspected rabies patients. DIF, Seller staining, MIT and RT-PCR were performed on the patients’ samples for the diagnosis of rabies. The ability of RT-PCR to detect rabies virus earlier as compared to other assays was tested both for reference virus as well as clinical isolates. Results: All samples taken ante-mortem were negative for DIF test. Six of 10 post-mortem brain tissues of the clinically suspected patients were positive both by RT-PCR and MIT, of these six, five were positive by DIF test and four were positive by Seller stain. RT-PCR could detect the rabies virus earlier as compared to DIF, both from clinical isolates and fixed rabies virus. Interpretation & conclusions: The present results showed 100 per cent sensitivity and specificity of RT-PCR as compared to 83.3 per cent of DIF and 66.7 per cent of Sellers stain for diagnosis of rabies. RT-PCR also detected rabies viral infection earlier as compared to conventional tests and can also be used on ante-mortem samples. Thus, the present study shows the usefulness of RT-PCR as an alternative to MIT for the confirmation of rabies diagnosis.

Newer trends in hanging death.

A total of 105 cases of deaths due to hanging were studied in a span of 5 years time. This present study throws some light on the emerging trends that Hanging is increasingly being adopted by a relatively younger age group who are mostly illiterate and of poor socio-economic status. Hanging being viewed as giving swift painless death and without much expenses and without arousing much suspicion, this mode is increasingly adopted to commit suicide. Dribbling saliva mark, which was a hallmark of Ante-mortem hanging, is increasingly seen in less number of cases. No incidence of fracture of Hyoid bone being seen in this study, due to the fact that most cases were of lower age group and also may be due to most of the victims adopting soft ligatures and low suspension points. Transverse tears in the Carotid arteries were not seen here and on subjecting to Histo-pathological study, we could detect disruption of intimal layer of Carotid artery. Effect of pressure on layers of skin were seen on Histo-pathological study showing vital reaction changes useful for labelling Ante-mortem hanging.

Linfadenite caseosa em ovinos deslanados abatidos em um frigorífico da Paraíba / Caseous lymphadenitis in slaughtered hair sheep in northeastern Brazil

Este trabalho teve como objetivo determinar a prevalência e distribuição de lesões da linfadenite caseosa em ovinos deslanados abatidos no município de Mulungu, Paraíba. Dos 1466 ovinos abatidos, 236 (15,9%) apresentaram lesões macroscópicas semelhantes à linfadenite caseosa. A prevalência foi maior em fêmeas, 17.9% (135/754) que em machos, 13,8% (101/732). As principais lesões estavam localizadas nos linfonodos pré-escapulares em 36,3% (97/268) dos ovinos, nos parotídeos em 22,4% (60/268) e no pré-crural em 20,9% (56/268). Somente 70 (26,1%) de um total de 268 lesões foram detectadas no exame ante mortem. Foram encontradas lesões em todos os lotes provenientes de diversos municípios da Paraíba, Pernambuco e Bahia. Das 51 amostras em que foi feito o cultivo bacteriológico, em 74,5% (43/51) foi isolado Corynebacterium pseudotuberculosis. Em 7,8% (4/51) foi isolado Staphylococcus aureus, em 2% (1/51) Escherichiacoli e 5,9% (3/51) amostras foram negativas. No exame histológico, em 11 linfonodos havia lesões da linfadenite caseosa caracterizadas por área de necrose central formada por lamelas concêntricas, com presença de grandes colônias bacterianas e focos de mineralização, rodeada por uma faixa de infiltrado inflamatório com macrófagos epitelióides e poucos neutrófilos. Na camada adjacente observaram-se linfócitos e plasmócitos e toda a lesão era delimitada por tecido conjuntivo fibroso. Nas outras 32 amostras foram observadas a maioria, mas não todas as lesões características da doença. Em 21 amostras foram encontradas células gigantes. As lesões histológicas dos linfonodos que foi isolado S. aureus foram semelhantes as da linfadenite caseosa. Conclui-se que as lesões histológicas de linfadenite caseosa são características, mas não patognomônicas, pois podem ser confundidas com lesões causadas por outros organismos piógenos e com tuberculose; portanto o isolamento bacteriológico é imprescindível para o diagnóstico definitivo da linfadenite caseosa.

The objective of this paper is to report the prevalence and distribution of lesions of caseous lymphadenitis (CL) in hair sheep slaughtered in an abattoir in the state of Paraíba, northeastern Brazil. From 1,466 sheep slaughtered, 236 (15.9%) had gross lesions similar to that of CL. The prevalence was higher in females, 17.9% (135/754), than in males, 13.8% (101/732). Ninety seven (36.3%) out of 268 lesions were localized in the prescapular lymph node, 22.4% (60/268) in the parotideal, and 20.9% (56/268) in the prefemoral. Only 70 (26.1%) out of 268 lesions suggestive of CL were detected during ante mortem examination. Caseous lymphadenitis-like lesions were observed in all flocks examined from different municipalities in the states of Paraíba, Pernambuco and Bahia. Cultures and histologic examination were performed in samples from 51 lesions. Corynebacterium pseudotuberculosis was isolated in 74.5% (43/51) samples, Staphylococcus aureus in 7.8% (4/51) samples, Escherichia coli in 2% (1/51), and in 5.9% (3/51) samples cultures were negative. Histologic findings in 11 lymph nodes showed all characteristic lesions of caseous lymphadenitis: central area of necrosis formed by concentric lamellae, with large bacterial colonies and mineralization, surrounded by an inflammatory exudate with epithelioid macrophages and few neutrophils; lymphocytes and plasma cells were observed in the next layer, and fibrous tissue was surrounding the whole lesion. Lesions in the other 32 lymph nodes showed most, but not all lesions characteristic of the disease. Giant cells were observed in 21 lymph nodes. Histologic lesions in lymph nodes from which S. aureus was isolated were similar as those of CL. These findings suggest that CL lesions are characteristic, but not pathognogmonic, because they can be confused with lesions caused by other pyogenic bacteria or tuberculosis; therefore bacteriologic examination is necessary for the diagnosis of the disease.

Role of dentist in person identification.

To reinforce awareness among dentists about their role in person identification and the importance of maintaining dental records of all their patients. The article reviews basic procedures of dental identification and some cases where dental identification played a key role in eventual identification of the person. Forensic odontology is an integral part of forensic sciences. Forensic dental identifications, especially in times of mass disasters, depend mainly on the availability of ante mortem dental records. It is the social responsibility of each and every dentist to maintain dental records of their patients for the noble cause of identification in the event of mass disaster.