ABSTRACT
Objective: A study was conducted to clarify the anti-influenza virus effect of agricultural, forest and fishery products in Ishikawa Prefecture. Method: The samples were prepared by both hot-water and ethanol extraction. The Madin-Darby canine kidney (MDCK) cells infected with PR8 strain were cultured in Dulbecco's Modified Eagle Medium（DMEM） containing extract for 24 hours and measured virus titer of supernatants by focus-forming reduction assay. Time-of-addition assay was performed to determine the inhibition stage in virus proliferation by the extract. The active ingredients were purified using a solid-phase extraction column (silica gel base C18). Results: We revealed that many products have anti-influenza virus activity. Especially, four products ( Matteuccia struthiopteris, Colocasia esculenta, Sagittaria trifolia, and Eleucine coracana) show strong activity and inhibit several stages in virus proliferation. Conclusion: We performed screening to 46 agricultural, forest and fishery products and found the four products ( Matteuccia struthiopteris, Colocasia esculenta, Sagittaria trifolia, and Eleusine coracana) inhibit virus proliferation strongly. In addition, the isolation and identification of the active ingredients contained in these remain as a future study.
ABSTRACT
Pandemic influenza is an acute respiratory infectious disease caused by influenza virus infection, which has the features of high incidence, widespread prevalence, and strong variability. For the prevention and treatment of influenza, Chinese materia medica (CMM) has obvious effects, relatively less toxic side effects, and it is popular and cheap, etc. Many scholars demonstrated the critical roles and mechanisms of CMM using the experiments of CMM against influenza virus. With investigating related literatures in recent years, this article summarizes the anti-influenza activities and mechanisms of actions of CMM, mainly the single herb and compound prescription, in order to provide idea and theoretical basis for future anti-influenza research.