Advances in the anti-ageing effects of GDF11 in blood / 中国输血杂志

Growth differentiation factor 11 (GDF11), a member of the transforming growth factor β superfamily, is widely expressed in multiple species such as human, mouse, rat, horse and sheep. Moreover, GDF11 is implicated in diverse biological functions and plays an important role in regulating anterior/posterior patterning, skeletal muscle regeneration, bone formation, vascular remodeling and neurogenesis. Recent studies have revealed that GDF11 in blood reverses age-related cardiac hypertrophy, skeletal muscle dysfunction and age-related cognitive decline, suggesting the potential value of GDF11 on anti-ageing. However, some other studies questioned the effects of GDF11 on anti-ageing. Herein, we highlighted structural characteristics of GDF11, advances in effects of GDF11 on anti-ageing, and the controversies of GDF11, to provide new insights for future studies on anti-ageing.

Effect of dimethylaminoethanol intradermal injection on collagen synthesis in an aging model of rats / 中华医学美学美容杂志

Objective:To evaluate the effects of cellulift ? administered intradermally by mesotherapy on collagen synthesis in D-galactose induced aging model of rats. Methods:The study was conducted between April and October in 2014 in the Department of Anatomy, Qindao University. 30 male rats were randomly allocated to three groups: aging treatment group, aging control group and normal group; each group had ten rats. Aging treatment group and the control group were subcutaneously injected with D-galactose prepared in saline 125 mg·kg -1·d -1 for 42 day. Normal group was injected with saline for 42 d with same method and dose. From the 18th day after shaving their hair, the dermis of two sides hip skin marked zone of aging treatment group were injected cellulift at a dose of 1 ml per week for 4 weeks. Meanwhile, the aging control group was administrated the same volume of saline with same method. In vivo skin collagen alterations were investigated by reflectance confocal microscopy 3 days after every treatment. Skin specimens were obtained in 42 days. In order to measure the dermal collagen density and dermal thickness, HE and Masson trichrome staining were performed, respectively. Immunohistochemical staining for TGFβ1 and proliferating cell nuclear antigen (PCNA) was performed. Also, the level of TGFβ1, Smad3, types Ⅰ and Ⅲ pro-collagen mRNA expression was assessed by real-time quantitative polymerase chain reaction. Results:As revealed by RCM, collagen density of aging treatment group increased gradually after treatments, while in aging control group it decreased with time. Measurement of dermal thickness, hydroxyproline content and TGFβ-1 mRNA and protein expression in treatment group increased significantly as compared with that in aging control group, but were significantly lower than that in normal group (F values were 25.45, 98.90, 37.94 and 21.35, respectively; P<0.05). Measurement of dermal collagen density, the mRNA expression of type I pre-collagen and Smad3 elevated over that of aging control group with significant difference (F values were 44.46, 29.54 and 10.01, respectively; P<0.05), and there was no difference between normal and aging treatment group ( P>0.05). The difference of PCNA expression between aging control and treatment groups was not significant ( P>0.05), and both were lower than normal group ( P<0.05) . Conclusions:Cellulift ? shows anti-aging effects by activating collagen synthesis and eventually causing dermal thickening. This effect is probably mediated by TGF-β1/Smad3 signaling pathway.

Contact Allergic Dermatitis to Cosmetics and Topical Anti-ageing Products

The main objective of this review article is to provide an update of the literature concerning delayedtypereactions or contact allergic dermatitis caused by cosmetic and anti-ageing ingredients. We scanfor tips on how to recognize these reactions from the history and clinical signs. The common allergensreported include fragrances and preservatives. Be aware of the new allergens. There is a need to patchtest with some of the heavy metals because of their presence in cosmetics but besides nickel, cobaltand occasional mercury, heavy metals do not frequently cause allergic contact dermatitis. They aredangerous because of their systemic toxicity on other internal organs. It is important to recognizethe source of the contact allergens that have been documented in various continents. Reports fromAsian countries (India, China, Korea, Singapore, Malaysia and Japan) have been included to increasethe awareness of cosmetic induced contact allergic dermatitis in this region. This knowledge uponreflections may assist us in our own daily practice.

Physical stability and clinical efficacy of Crocodylus niloticus oil lotion

ABSTRACT The stability and the anti-ageing, skin hydrating and anti-erythema effects of a commercialized Crocodylus niloticus Laurenti, 1768, Crocodylidae, oil lotion was determined. The lotion was stored at controlled conditions over six months during which several stability tests were performed. For the clinical efficacy studies lotion was applied on volar forearm skin (female volunteers) and compared to a liquid paraffin-containing reference product. Skin hydrating and anti-ageing effects were determined with a Corneometer®, Cutometer® and Visioscan®, following single (3 h) and multiple applications (12 weeks). The Vapometer® and Mexameter® were utilized to determine this lotion's anti-erythema effects on sodium lauryl sulfate irritated skin. The lotion demonstrated good stability over 6 months. The reference product increased skin hydration and decreased skin wrinkles to a larger extent than the C. niloticus lotion after a single application, whereas the C. niloticus lotion decreased skin scaliness better than the reference product. During the long-term study, the reference product overall increased skin hydration more than the C. niloticus lotion, whereas C. niloticus lotion increased skin elasticity to a larger extent than the reference product. C. niloticus lotion increased skin wrinkles and decreased skin scaliness over 12 weeks. Compared to non-treated, irritated skin, C. niloticus lotion demonstrated some potential anti-inflammatory characteristics.

O acesso aos campos elísios: a promoção da saúde ampliada e as tecnologias de melhoramento em busca da longevidade (e da imortalidade) / Access to the elysian fields: amplified health promotion and enhancement technologies in search of longevity (and immortality)

São abordadas e discutidas as tecnologias de melhoramento e sua meta de vender a possibilidade (real ou virtual) de manter e proporcionar aparência de juventude, longevidade e até imortalidade aos seres humanos como modelo de construção da noção de si mesmo. Emprega-se a ideia de "promoção de saúde ampliada" tanto no sentido de intensificação dos discursos sustentando comportamentos saudáveis como alegoria fotográfica no sentido de ampliar a sua imagem e permitir uma visão mais aproximada de detalhes políticos, ideológicos e mercantis das suas proposições. A partir de uma tipologia das ciências contra o envelhecimento feita por John Vincent em: cosméticas, médicas, biológicas e imortalistas, são enfocados os dois últimos itens e suas implicações. Ao final, propõe-se um enfoque analítico da questão, destacando estratégias biopolíticas para lidar com a finitude humana através de enfoques preemptivos sob a égide da hiperprevenção e a busca de um tipo de felicidade como autossatisfação pessoal que necessita de tecnologias de melhoramento para ser alcançada.

In this article, we discuss enhancement technologies and their goal to sell the possibility (actual or virtual) of keeping and providing appearances of youth, longevity and even immortality for human beings as a construction model of the notion of self. We employ the idea of 'amplified health promotion' in the sense of intensification of discourses supporting healthy behaviours and also as a photographic allegory, in the sense of amplifying its image in order to allow a closer view of the political, ideological and mercantile details of its proposals. Based on a typology of the anti-ageing sciences, proposed by John Vincent as cosmetic, medical, biological and immortalist, we deal with the latter two and their implications. Finally, we propose an analytical focus on the subject, emphasizing biopolitical strategies to deal with human finitude through preemptive approaches with the support of hyper-prevention, and the search of a kind of happiness as personal self-fulfillment that needs enhancement technologies to be reached.

Determination of phytochemical constituents and antioxidant activity of Rasayana, Triphala and antiageing herbal plants.

Antioxidants possess the ability to protect the body from damage caused by free radical induced oxidative stress and have antiageing property. ‘Theinga Dara Thanhitar’ book, written by Ashin Nagathein described that ‘Rasayana and Triphala’ drugs have the ability to prevent ageing and illness. This study was carried out to investigate the antioxidant activities of commonly used ingredients of ‘Rasayana’ such as Streblus asper Lour. (Okhne), Cassia fistula Linn. (Ngu), Tinospora cordifolia Miers. (Sindone-Ma-Nwe), Asparagus racemosus Willd. (Shin-Matet), Boerhavia diffusa Linn. (Payan-Nawa), and ‘Triphala’ such as Phyllanthus emblica Linn. (Ze-Phyu), Terminalia chebula Retz. (Phan-Ga) and Terminalia belerica Roxb. (Thit-Saint). Their absorbances were evaluated using 1,1-diphenyl -2-picrylhydrazyl (DPPH) assay method by using spectrophotometer. Yield percents of these plants were ranging from 4.05% to 28.6%. The watery extracts of Okhne, Ngu, stem of Sindone-Ma-Nwe, tubor of Payan-Nawa, Ze-Phyu, Phan-Ga, and Thit-Saint contain phenols and tannins which have free radical scavenging activities. It was found that the bark and stem of Okhne, tubor of Payan-Nawa and ‘Triphala’ had the least IC50 value i.e. the best antioxidant potency. Therefore, the findings from this study supported the experience-based findings of antiageing activities in ‘Triphala’ and ‘Rasayana’ to be evidence-based.

Formulation and in vitro evaluation of the topical antiageing preparation of the fruit of Benincasa hispida.

Ageing is the phase of gradual decline of body efficiency and metabolic activities after reaching a maturity stage. Free radicals cause oxidative alterations in collagen, elastin material and changes in membrane characteristics and induce polymerization reactions. Use of topical antioxidants can overcome some of these effects and retard actinic ageing. Herbal products are popular due to their minimum risk of side-effects with maximum efficacy. The present study was undertaken to evaluate the antiageing potential of Benincasa hispida fruit extract as not many scientific studies have been carried out to explore its utility as skin renewal enhancer and as an antioxidant. After removing the outer layer and the seeds, the fruit pulp was dried. The dried fruit pulp was extracted successively with petroleum ether, chloroform, ethyl acetate and methanol by Soxhlation for 2 days. Methanol was recovered under vacuum and a dry extract was obtained (yield 4.2% w/w), which was stored in a desiccator. Suitable topical cream base for effective carriage of fruit extract was developed and its in vitro evaluation for skin renewal activity was tested by application to the stratum corneum of human cadaver skin and by dansyl chloride fluorescence method. The results show that the cream prepared from Benincasa fruit extract may prove as an antiageing preparation and can be used for retarding the symptoms of ageing.