ABSTRACT
Objective To study the antibacterial components of chloroform and ethyl acetate extracts of the traditional Chinese medicine Artemísia anómala S. Moore. Methods The chloroform and ethyl acetate extracts of Artemísia anómala S. Moore was isolated by silica gel column chromatography. The antibiotic tests of the different isolated fractions in vilro were performed by using Kriby-Bauer method and broth dilution method. The compound structures of the significantly active components were identified by the method of chemical colored-reaction and spectrum analysis. Results Multiple components of chloroform and ethyl acetate extracts of Artemísia anómala had different degrees of antibacterial activities for common pathogenic strains in clinic. The structures of significantly active components were identified as: apigenin, eupatilin, and caffeic acid; eupatilin had the strongest antimicrobial activity for Standard Slaphylococcus aureus (ATCC25923) and Standard Staphylococcus aureua (ATCC29213), with the minimal bactericidal concentrations being both 0. 062 5 mg/mL. Conelusion The proposcd method in the present study is simple and quick; it can aceurately and cffectively obtain the antibacterial components of the chloroform and ethyl acetate extracts of Artemísia anómala. This study is the first to report the antibacterial activity of eupatilin.
ABSTRACT
Objective To investigate the clinical application significance of flow cytofluroometric(FCM ) antibiotic susceptibility test for A .baumannii in vitro .Methods The sensitivity to Ampicillin/Sulbactam ,Levofloxacin ,Meropenem ,Cefotaxime of an Esch‐erichia coli standard strain and 66 isolates of A .baumannii were tested with FCM by using propidium iodide as a fluorescent probe . The survival rates of the bacteria in the culture after treatment with different dosages of the antibiotics were determined according to the fluorescence strength .The MIC value of the antibiotics against the 66 strains were judged with FCM ,compared with microdi‐lution and VITEK methods .Results The antibiotic resistant strains number of sultamicillin ,levofloxacin ,meropenem and cefo‐taxime sodium were 35 ,30 ,13 and 38 respectively in flow cytofluorometric antibiotic sensitivity test(FCST) of 66 strains of A .bau‐mannii .There was no significant difference(P>0 .05) compared with the antibiotic susceptibility results by the methods of VITEK instrument and microdilution by measuring with χ2 test respectively .Conclusion The established method of FCST for A .baumannii is suitable for dectecting bacterial drug‐sensitivity ,which is more rapid ,accurate and objective .