ABSTRACT
The antidiarrhoeal activity of Cryptocoryne spiralis rhizomes extract (250, 500, 750 mg/kg, po) was evaluated using faecal excretion, castor oil-induced diarrhoea, small intestinal transit, intestinal fluid accumulation, gastric emptying and PGE2 induced enteropooling models in rats. In addition, various biochemical estimations, histopathological studies and antibacterial evaluations on strains responsible for diarrhoea were also performed. The results illustrated a significant reduction in normal faecal output rate after 5th and 7th h of treatment, while castor oil-induced diarrhoea model depicted a protection of 55.44% at same dose level from diarrhoea. The other models except, gastric emptying test demonstrated more pronounced effect at same dose level. A significant inhibition in nitric oxide, increase in carbohydrates, protein, DNA, Na+ and K+ level with minimum degeneration of colonic fibrous tissues and potent antibacterial activity were also observed. The antidiarrhoeal potential of C. spiralis may be as a result of antimotility and antisecretory type effect mediated through nitric oxide pathway.
Subject(s)
Animals , Antidiarrheals/administration & dosage , Antidiarrheals/chemistry , Araceae/chemistry , Castor Oil/toxicity , Diarrhea/chemically induced , Diarrhea/drug therapy , Diarrhea/pathology , Humans , Metabolic Networks and Pathways/drug effects , Nitric Oxide/metabolism , Plant Extracts/administration & dosage , Plant Extracts/chemistry , Rats , Rhizome/chemistryABSTRACT
Punica granatum (Family: Punicaceae) is a widely consumed fruit in Bangladesh locally known as Anar or Bedana. Traditionally the plant is used to treat diarrhoea, dysentery, hemorrhage, tuberculosis and chronic periodontitis. The present study was undertaken in order to justify and authenticate the traditional use of rind of P. granatum fruit in diarrhoea. Methanolic extract of the rind of the fruit was tested in castor oil- and magnesium sulfate-induced diarrhoea in mice at the doses of 200 and 400 mg/kg body weight. The results of the present study revealed signifi-cant antidiarrhoeal activity (p<0.001) in both animal models. The extract inhibited 31.25% defaecation at the dose of 200 mg/kg and 53.75% at the dose of 400 mg/kg in castor oil-induced diarrhoea while standard drug loperamide inhibited 71.25% defaecation. In magnesium sulfate-induced diarrhoea the inhibition of defaecation was 45.71 and 57.14% at the doses of 200 and 400 mg/kg respectively while loperamide inhibited 64.29% defaecation. The antidiarr-hoeal effect of the extract was concentration dependent in both castor oil-induced diarrhoea and magnesium sulfate-induced diarrhoea. Phytochemical screening of the extract revealed the presence of flavonoids and alkaloids that may play key role in its antidiarrhoeal activity. The results of the present study validate the folkloric use of the rind of P. granatum in the treatment of diarrhoea.
ABSTRACT
Punica granatum (Family: Punicaceae) is a widely consumed fruit in Bangladesh locally known as Anar or Bedana. Traditionally the plant is used to treat diarrhoea, dysentery, hemorrhage, tuberculosis and chronic periodontitis. The present study was undertaken in order to justify and authenticate the traditional use of rind of P. granatum fruit in diarrhoea. Methanolic extract of the rind of the fruit was tested in castor oil- and magnesium sulfate-induced diarrhoea in mice at the doses of 200 and 400 mg/kg body weight. The results of the present study revealed signifi-cant antidiarrhoeal activity (p<0.001) in both animal models. The extract inhibited 31.25% defaecation at the dose of 200 mg/kg and 53.75% at the dose of 400 mg/kg in castor oil-induced diarrhoea while standard drug loperamide inhibited 71.25% defaecation. In magnesium sulfate-induced diarrhoea the inhibition of defaecation was 45.71 and 57.14% at the doses of 200 and 400 mg/kg respectively while loperamide inhibited 64.29% defaecation. The antidiarr-hoeal effect of the extract was concentration dependent in both castor oil-induced diarrhoea and magnesium sulfate-induced diarrhoea. Phytochemical screening of the extract revealed the presence of flavonoids and alkaloids that may play key role in its antidiarrhoeal activity. The results of the present study validate the folkloric use of the rind of P. granatum in the treatment of diarrhoea.
ABSTRACT
Objective:To evaluate the antidiarrhoeal property of methanol extract of the leaves of Rauwolfia serpentina (R. serpentina) in experimental diarrhoea induced by castor oil in mice. Methods:Doses of 100, 200 and 400 mg/kg R. serpentina leaf methanol extracts were administered to castor oil induced diarrhoea mice to determine its antidiarrhoeal activity. Results: All doses of the extract and the reference drug atropine sulphate (3 mg/kg, i.p.) produced a dose-dependent reduction in intestinal weight and fluid volume. The extracts also significantly reduced the intestinal transit in charcoal meal test when compared to diphenoxylate Hcl (5 mg/kg, p.o.). Conclusions: The results show that the extract of R. serpentina leaves has a significant antidiarrhoeal activity and supports its traditional uses in herbal medicine.
ABSTRACT
Desmostachya bipinnata root has been used in the Indian traditional system of medicine for treatment of diarrhoea and dysentery. The antidiarrhoeal effect of both alcoholic and aqueous extracts of the roots of Desmostachya bipinnata were studied in rats against castor oil induced diarrhoea and charcoal meal test at the doses of 200 and 400 mg/kg body weight. The alcoholic extract and to a lesser extent aqueous extract significantly reduced the weight of the faces and decreased the propulsion of charcoal meal through the gastrointestinal tract. The phytochemical screening of the extracts showed the presence of alkaloids, glycosides, flavonoids, tannins, phytosterol, terpenoids, polyphenolics, protein and carbohydrates. These results may support the fact that this plant is used traditionally to cure diarrhoea.
La raíz de Desmostachya bipinnata ha sido utilizada en el sistema tradicional de medicina Hindú para el tratamiento de diarrea y disentería. El efecto antidiarreico de los extractos alcohólicos y acuosos de los extractos de la raíz de Desmostachya bipinnata fueron estudiados en ratas, utilizando la diarrea inducida por aceite de castor y el ensayo de la prueba por carbón en dosis de 200 y 400 mg/kg de peso corporal. El extracto alcohólico y en menor grado, el extracto acuoso, redujeron significativamente la propulsión de carbón a través del tracto gastrointestinal. El análisis de los extractos mostrarón la presencia de alcaloides, glicósidos, flavonoides, taninos, fitoesterol, terpenoides, polifenoles, proteínas y carbohidratos. Estos resultados pueden apoyar el hecho de que esta planta sea usada tradicionalmente para curar la diarrea.