ABSTRACT
Objective: To investigate the effect of cell adhesion molecule CD44v6 on biological behavior of bladder cancer cell line T24. Methods: The expressions of CD44v6 protein in bladder transitional epithelial cells and bladder cancer T24 cells were detected by immunocytochemistry. MTT assay was used to detect the growth inhibitory rate of T24 cells treated with different concentrations of anti-CD44v6 monoclonal antibodies for 24, 48 and 72 h, respectively. After T24 cells were treated with anti-CD44v6 monoclonal antibodies, the apoptosis was detected by flow cytometer, the expressions of apoptosis-related markers Bcl-2 and Bax were determined by immunohistochemistry, and the change of migration ability of T24 cells was detected by Transwell migration test. Results: CD44v6 was expressed in T24 cells, but rarely expressed in bladder transitional epithelial cells. MTT assay demonstrated that anti-CD44v6 monoclonal antibodies had inhibitory effects on the proliferation of T24 cells (P <0.01). The apoptosis rate of T24 cells was increased with an induction of anti-CD44v6 monoclonal antibodies (P <0.01). The expression level of Bax protein was up-regulated, while the expression level of Bcl-2 protein was down-regulated in T24 cells (P <0.01). The migration of T24 cells was inhibited after treatment with anti-CD44v6 monoclonal antibodies (P <0.05). Conclusion: Anti- CD44v6 monoclonal antibodies can inhibit the abilities of proliferation and migration of T24 cells, and also induce apoptosis. The mechanism may be related with the expression regulation of Bcl -2 and Bax genes. Copyright© 2012 by TUMOR.
ABSTRACT
Objective To investigate the importance of PTEN protein and CD44v6 detection to the prediction of laryngeal carcinoma differentiation and cervical lymph node metastasis. Methods The expressions of PTEN protein and CD44v6 were detected by immunohistochemical SP method in 97 specimens with laryngeal carcinoma and 97 specimens with a distance over 2.5 centimeters from laryngeal carcinoma. The relations between them and their clinical significance to the laryngeal squamous cell carcinoma above Glottis by the statistical analyses tools were revealed. Results The positive expression rates of PTEN protein were respectively 55.67 %, 100 % in laryngeal carcinoma and adjacent carcinoma tissue. There was a significant difference between them statistically (P<0.01). The positive expression rates of PTEN protein are notable difference among the high, middle and low differentiation of laryngeal carcinoma, but not between the positive metastasis of lymph node of neck and the negative one(P >0.05). For the expression of CD44v6, there were notable statistical difference not only among the different differentiation of laryngeal careinoma, but also between the positive metastasis of lymph node of neck and the negative one. The low expressions of PTEN protein and the high expression of CD44v6 in the same carcinoma cell has significant negative correlation (P< 0.05). Conclusion PTEN protein may have the importance to the differentiation of laryngeal carcinoma, but no means to cervical lymph node metastasis. CD44v6 may have great importance to both.