Effects of microbe-derived antioxidant on sleep and antioxidant ability in the rat / 中国比较医学杂志

Objective To investigate the effect of different doses of microbe?derived antioxidant on sleep and antioxidant ability in mice. Methods Sixty male Kunming mice with similar body weight were randomly divided into 4 groups. The control group received normal saline, and the experimental groups received microbe?derived antioxidant in a dose of 0?5 g/kg bw, 1?0 g/kg bw or 1?5 g/kg bw once per day, respectively. The experiment period was 30 days. At the end of experiment, the mice of each group were intraperitoneally injected sodium pentobarbital to induce sleep. The mice fall sleep was judged by righting reflex. After the test of sleep, blood was taken for detection of serum antioxidant ability. Results Compared with the low dose and high dose groups, the middle dose group showed a significantly prolonged sodium pentobarbital?induced sleeping time (P<0?05). Compared with the control group, low and high dose groups, the middle dose group had highly significantly increased GSH?Px activity ( P <0?01 ) and significantly increased content of SOD. Under these conditions, the middle dose group reduced both the contents of MDA and 8?ISO?PGF2α ( P<0?05 ) compared with the control group. Conclusions Our results suggest that microbe?derived antioxidant exerts effect on sleep and antioxidant ability in rats. Supplement of 1?0 g/kg bw/d shows the most significant effects.

Screening of Antioxidant Active Components of Schisandra chinensis / 中国药房

OBJECTIVE:To screen antioxidant active components of Schisandra chinensis. METHODS:The orthogonal test was adopted to optimize extraction technology using DPPH free radical scavenging activity(IC50)as index and ethanol volume frac-tion,material-liquid ratio and extraction time as factors,and the verification test were made. The fractions(SC-0,SC-10,SC-30, SC-50,SC-70,SC-95) were made by extracting and purifying S. chinensis with macroporous resin with water and 10%,30%, 50%,70%and 95%ethanol. With IC50 and total antioxidant capacity(determined by ABTS method)as indexes(vitamin C as pos-itive control),the antioxidant active components of S. chinensis were optimized. The contents of 5 kinds of lignan in different posi-tions of S. chinensis were determined by HPLC. RESULTS:The optimal extraction condition of S. chinensis was as follows as 60% ethanol,material-liquid ratio of 1∶14,extracting for 2.0 h. The average IC50 of DPPH free radical scavenging activity was 23.81 mg/ml(RSD=0.52%,n=3)in verification test. SC-0 did not have antioxidant abilities. DPPH free radical scavenging activi-ty of those components (ie. the IC50 value from low to high) were in the following order of positive control>SC-50>SC-30>SC-95>SC-70>SC-10;total antioxidant ability of them were in the following order of SC-50>positive control>SC-30>SC-70>SC-95>SC-10;the contents of 5 types of lignan in different components were in the following order of SC-70>SC-50>SC-95>SC-30. CONCLUSIONS:The antioxidant active component of S. chinensis is 50%ethanol eluate.

Study on Antioxidant Effects of Different Extract Parts from Aquilaria sinensis Leaves in vitro / 中国药房

OBJECTIVE:To investigate antioxidant effects of different extract parts from Aquilaria sinensis leaves. METH-ODS:A. sinensis leaves were extracted by reflux extraction with petroleum ether,ethyl acetate,absolutely ethanol,water to obtain corresponding parts (AGP,AGE,AGA and AGW parts). The ability of different extracts eliminating ABTS+ free radical,DPPH free radical and O2-free radical and reducing Cu2+ were investigated. IC50 was also calculated. Vitamin C(VC)was used as positive control in above tests. RESULTS:Those parts eliminated above free radicals and reduced Cu2+ in concentration-dependant manner, among which maximal elimination rate of AGA and AGW parts to ABTS+ free radical were 100% and 81.07%(IC50=14.89,23.12 mg/L)and higher than that of VC(88%);those of other parts were less than 50%. The maximal elimination rate of AGA parts to DPPH free radical was 67.74%(IC50=47.96 mg/L),but still lower than that of VC(91.44%);the elimination rates of other parts were all weak and lower than 40%. The maximal elimination rates of AGA and AGW parts to O2-free radical were 67.79% and 61.79%(IC50=393.01,421.04 mg/L),but still lower than that of VC(88%);the elimination rates of other parts were all lower than 33%. The maximal reduction percentage of AGP,AGE,AGA and AGW parts to Cu2+ were all higher than 50%(IC50=55.99, 40.27,25.44,31.06 mg/L,respectively),but still lower than that of VC (100%). CONCLUSIONS:Different extract parts from A. sinensis leaves have antioxidant activity in vitro,and that of AGA is the strongest.

Research on the oxidation damage of sodium arsenite on rat lung / 中国职业医学

OBJECTIVE: To explore the oxidation damage of sodium arsenite( NaAsO_2) drinking exposure on rat lung.METHODS: Thirty-two specific pathogen free healthy adult male SD rats were randomly divided into 4 groups: control group,low-,medium- and high-dose groups,with 8 rats in each group. The 3 arsenic exposure groups were intoxicated by NaAsO_2 with mass concentrations of 10. 00,100. 00 and 1 000. 00 μg / L in drinking water,respectively,while the control group was given ultrapure water for free drinking. All the rats were sacrificed after 28 days. The total number of white blood cells and the cell death rate in bronchoalveolar lavage fluid( BALF) were measured. The level of reactive oxygen species( ROS),nicotinamide adenine dinucleotide phosphate( NADPH),the antioxidant ability and the nitric oxide( NO) level in BALF were measured by the enzyme-linked immunosorbent assay. RESULTS: The total number of white blood cells in BALF in medium-dose group was higher than those of the control group and high-dose group( P < 0. 05).The cell death rate of BALF in high-dose group was higher than those of the other groups( P < 0. 01). The ROS levels in BALF of rat lung in 3 exposure groups were higher than that of the control group( P < 0. 01). The NADPH levels in medium- and high-dose groups were higher than those of the control group and low-dose group( P < 0. 05). The total antioxidant ability in medium- and high-dose groups were lower than that of the control group( P < 0. 01). The NO levels in medium- and high-dose groups were higher than that of the control group( P < 0. 05). CONCLUSION: The NaAsO_2 exposure in rats could lead to high expression of oxidase ROS by activating NADPH followed by increasing NO level,resulting in imbalanced organism oxidation and anti-oxidation system and causing oxidative stress injury in tissues.

Antioxidant ability and radiosensitivity in malignant transformed human bronchial epithelial cell line BEP2D induced by α-particle irradiation / 中华放射医学与防护杂志

Objective To investigate the antioxidant ability and radiosensitivity in the malignant transformed human bronchial epithelial cell line BEP2D induced by α-particle exposure.Methods Glutathione Peroxidase (GPX),Catalase (CAT) and Glutathione (GSH) assay kits were employed to detect GPX and CAT enzyme abilities and the levels of GSH in BEP2D,RH21 ( passage 21 of α-particle-irradiated BEP2D cells),and BERP35T-1 cells (derived from nude mice bearing malignant transformed cells generated from cells of passage 35 of α-particle-irradiated BEP2D cells).MTT assay were used to test the growth rate of BEP2D,RH21 and BERP35T-1 cells treated with 0,30,60,90,120,and 150 μmoL/L H2O2.Colony-forming test and MTT assay were used to examine the cell survival fraction and the growth rate of BEP2D,RH21 and BERP35T-1 cells exposed to 0,2,4,and 8 Gy of γ-rays,respectively.Results GPX and CAT enzyme activities in RH21 and BERP35T-1 cells were obviously lower than in BEP2D( t = 5.75-67.92 ,P ＜ 0.05 ).CAT enzyme activity in BERP35T-1 was lower than that in RH21 cells (t =22.25 ,P ＜0.01 ).Compared to BEP2D cells,decreased level of GSH was detected in BERP35T-1 cells(t = 7.76,P ＜ 0.05 ),but there was no change in RH21.After treatment with 30,60,90,120,and 150 μmol/L H2O2,the growth rates of BEP2D were all higher than those of BERP35T-1 cells(t = 10.37-58.36,P ＜0.01 ).Meanwhile,the growth rates of BEP2D were all higher than those of RH21 cells after treatment with 60,90,120,and 150 μ mol/L H2O2 (t = 29.90-84.68,P ＜ 0.01 ).In addition,compared to BEP2D cells,decreased cell survival fraction and growth rate of BERP35T-1 cells were observed after irradiation with 2,4,and 8 Gy of y-rays ( t = 5.87-34.17,P ＜ 0.05 ).The cell survival fraction and growth rate of RH21 were all lower than those of BEP2D cells at 4 and 8 Gy post-irradition( t =6.33- 45.00,P ＜ 0.05 ).Conclusion The function of antioxidant system decreased in the α-particleinduced transformed cells,which could contribute to the acceleration of cellular malignant transforming process and radiosensitivity.

Lipid peroxidation and total antioxidant ability in venous plasma and amniotic fluid of pregnant women with preterm premature rupture of membranes / 대한산부인과학회지

OBJECTIVE: To evaluate their roles in the pathophysiology of preterm premature rupture of membranes (PPROM), we checked interleukin (IL)-6, lipid peroxide, oxygen-radical absorbance capacity (ORAC) and antioxidant vitamin in the venous plasma and amniotic fluid of women with PPROM. METHODS: Venous plasma and amniotic fluid was taken from 20 normal pregnant women and 20 PPROM pregnancy women. IL-6 levels was determined by enzyme-linked immunosorbent assay. Lipid peroxide levels were measured by thiobarbituric acid reaction. The ORAC levels and the antioxidant levels were measured by Cao's method and by high performance liquid chromatography. RESULTS: The IL-6 levels in the venous plasma and amniotic fluid of PPROM pregnancy women were significantly higher than normal pregancy (P<0.01, P<0.01). The lipid peroxide levels in the venous plasma and amniotic fluid of PPROM pregnancy women were significantly higher than normal pregancy (P<0.01, P<0.01). The ORAC values in the venous plasma and amniotic fluid of PPROM pregnancy women were significantly higher than normal pregancy (P<0.01, P<0.01). The ORAC values/lipid peroxide levels in the venous plasma and amniotic fluid of PPROM pregnancy women were significantly higher than normal pregancy (P<0.01, P<0.01). The ascorbic acid levels in the venous plasma and amniotic fluid of PPROM pregnancy women were significantly higher than normal pregancy (P<0.05, P<0.01). CONCLUSION: This result suggest that the increased inflammatory bioactivity, increased lipid peroxidation and decreased antioxidant activity may be involved in the pathophysiology of PPROM. Low levels of ascorbic acid appears to be an important determinant of PPROM.

Effect of vitamin C and E on the lipid peroxide, antioxidant ability, and antioxidant vitamin levels in maternal and umbilical venous plasma / 대한산부인과학회지

OBJECTIVE: This controlled trial investigated the effect of supplementation with vitamins C and E on the lipid peroxide, antioxidant ability, and antioxidant vitamin levels in full term maternal and umbilical venous plasma. METHODS: Forty pregnant women were randomized in this study. They were nulliparous or multiparous women with history of preeclampsia or preterm premature rupture of membranes (PPROM). Twenty women were given vitamin C (1,000 mg/day) and vitamin E (400 IU/day) from 15~20 gestational weeks to delivery. The others were not given, as a control group. Maternal venous blood was obtained before vitamin supplementation and just before delivery. Umbilical venous blood was obtained after delivery. Lipid peroxide level and oxygen-radical absorbance capacity (ORAC) were measured by thiobarbituric acid reaction and Cao's method, respectively. Ascorbic acid, uric acid, beta-carotene, retinol, alpha-tocopherol, and gamma-tocopherol were measured by high performance liquid chromatography. Results: Supplementation with vitamins C and E was associated with decrease in lipid peroxide levels and increase in ORAC values and alpha-tocopherol levels in maternal venous plasma. And it was also associated with decrease in lipid peroxide levels and increase in ORAC values and alpha-tocopherol levels in umbilical venous plasma. CONCLUSION: Supplementation with vitamins C and E may be beneficial in the prevention of oxidant-antioxidant imbalance origin diseases such as preeclampsia, PPROM. And it also may affect antioxidant ability of the fetus.

Changes of interleukin-6, tumor necrosis factor-alpha, and total antioxidant ability in the umbilical venous plasma of preterm birth with or without premature rupture of membranes / 대한산부인과학회지

OBJECTIVE: To compare interleukin-6 (IL-6), tumor necrosis factor-alpha(TNF-alpha), oxygen-radical absorbance capacity (ORAC), and antioxidant vitamin levels in the umbilical venous plasma of preterm labor with intact membranes (PTL) and preterm premature rupture of membrane (PPROM) with that of full term normal pregnancy (NP) and to evaluate their roles of pathophysiology in preterm labor and PPROM. METHODS: Umbilical venous blood samples were collected from women with PTL (n=30), PPROM (n=30) and NP (n=30). IL-6 and TNF-alpha were measured by enzyme-linked immunosorbent assay. Lipid peroxide levels were measured by thiobarbituric acid reaction. The ORAC was mesured by Cao's method. Antioxidant vitamin levels were measured by high performance liquid chromatography. RESULTS: IL-6 and TNF-alpha levels in the umbilical venous plasma of PPROM were significantly higher than that of PTL and NP (3.28+/-0.31 vs. 2.84+/-0.19 vs. 2.79+/-0.22 pg/ml, p<0.05), (2.30+/-0.27 vs. 1.64+/-0.23 vs. 1.40+/-0.25 pg/ml, p<0.01). Lipid peroxide levels in the umbilical venous plasma of PPROM were significantly higher than that of NP and PTL (2.78+/-0.27 vs. 2.54+/-0.32 vs. 2.24+/-0.24 nmol/mg protein, p<0.01). ORAC levels in the umbilical venous plasma of PTL and PPROM were significantly lower than that of NP (967425.4+/-98.99 vs. 965165.8+/-91.20 vs. 1011328.5+/-85.96 U/ml, p<0.05). Ascorbic acid levels in the umbilical venous plasma of PPROM were significantly lower than that of NP and PTL (125.6+/-2.33 vs. 158.3+/-3.08 vs. 221.7+/-2.82 nmol/ml, p<0.01). CONCLUSION: Increase in inflammatory cytokines (IL-6, TNF-alpha) and imbalance of increased lipid peroxidation and decreased antioxidant activity in umbilical venous blood may be involved in the pathophysiology of PPROM.

STUDY ON THE PROTECTIVE EFFECTS OF POLYPEPTIDE-Fe ON ACUTE HEPATIC INJURY IN MICE / 营养学报

Objective: To study the protective effects of polypeptide-Fe on acute hepatic injury induced by CCl4 in mice. Method:Mice model of acute hepatic injury was set up by CCl4 ig (50mg/kg?d ). and fed with polypeptide-Fe 67.5 mg/kg?d, 675 mg/kg?d and 2 025 mg/kg?d for 30 d respectively. The effects on serum ALT, AST, and ALP and liver TG, TC, GSH, MDA, and SOD avtivities were observed. Results: Polypeptide-Fe decreased the activities of ALT, AST, and ALP in serum and the levels of TG, TC, and MDA in liver, and increased the level of GSH in liver. Middle dosage group of polypeptide-Fe was the most effective one. Conclusion:Polypeptide-Fe showed significant antioxidant ability in protecting acute hepatic injury induced by CCl4 in mice.