ABSTRACT
This study was performed to examine the feeding effects of Angelica keiskei Koidz (AK) and its processed products on serum, liver, and body fat content and the expression of antioxidant genes in rats fed a high fat diet. AK and its processed products were added at 3-5% to a high fat diet and fed to adult rats for 6 weeks. In experiment 1 (EXP 1), the rats were fed with one of six diets including a control diet (normal fat), high fat diet (HF), and HF + AK additives groups (four groups). In experiment 2 (EXP 2), the rats were separated into three groups of HF, HF + AK whole leaves, and HF + fermented juice (FS) + squeeze (SA). Body weight was not different among the groups in either experiment. The liver weight was lower in the FS and SA groups compared to that in the other groups (P < 0.05). Serum luteolin was higher in the AK and processed products groups compared to that in the HF group (P < 0.05). Gene expression of the antioxidative enzymes catalase and glutathione-s-reductase in the liver was higher in the AK processed products group than that in the other groups (P < 0.05). The results suggest that the intake of AK and its processed products increased the expression of antioxidant enzymes in animals fed a high fat diet, reduced hepatic cholesterol content, and increased the effective absorption of luteolin.
Subject(s)
Adult , Animals , Humans , Rats , Absorption , Adipose Tissue , Angelica , Body Weight , Catalase , Cholesterol , Diet , Diet, High-Fat , Gene Expression , Lipid Metabolism , Liver , LuteolinABSTRACT
Objective To explore the effect of hyperbaric oxygenation (HBO) on generation of free radicals and the permeability of blood-brain barrier(BBB) during cerebral ischemia-reperfusion(CIR). Methods Three hundred and twenty Kunming mice were randomly divided into four groups: a sham operation group, a HBO group, a CIR group, and a HBO + CIR group, with 80 mice in each group. Conscious mice were used to establish the CIR model, and 0.25MPa (ATA) HBO were applied 5 times after operations. The activities of SOD, CAT, GSH-PX and the concentration of MDA, EB in the cerebral hippocampal tissues in each group were measured with colorimetry. The cerebral hippocampal tissues were harvested and processed, then observed and compared with transmission electron microscopic observation. Results Compared with the sham operation group, the activities of antioxidation enzymes (GSH-PX,SOD, CAT) in CIR group decreased significantly (P
ABSTRACT
The experimental study of the lesions of red cell membrane caused by peroxi-dation was performed,indicating that having oxidized by superoxide anions,thered cell lesions in appearance were that there was formed the high molecular pro-tein cross-linked polymer,and the conjugated diene level was increased.And thesimilar feature of oxidation lesions was observed in the stored red cells.It wasshowed that antioxidation enzymes could inhibit the forming of protein cross-linkedpolymer and have certain protecting effect on red cell membrane being subject tolesion.