ABSTRACT
OBJECTIVE: To develop an RP-HPLC method for simultaneous determination of rosmarinic acid, tilianin, luteolin-7-O-β-D-glucuronide, apigenin-7-O-β-D-glucuronide, and diosmetin-7-O-β-D-glucuronide in different parts ofDracocephalum moldevica L. METHODS: The five constituents were measured on a Shim-pack ODS column(4.6 mm×250 mm, 5 μm)with gradient elution of acetonitrile (A)-0.5% formic acid aqeous solution (B) (0-30 min, 17%A;30-60 min, 17%-28%A; 60-70 min, 28%A) at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 330 nm, and the column temperature was maitained at 35℃. RESULTS: The linear ranges of rosmarinic acid, tilianin, luteolin-7-O-β-D-glucuronide, apigenin-7-O-β-D-glucuronide, and diosmetin-7-O-β-D-glucuronide were 4.2-126 μg·mL-1 (r=0.999 2), 7.84-235.2 μg·mL-1 (r=0.999 3), 3.048-91.44 μg·mL-1(r=0.999 4), 1.472-44.16 μg·mL-1(r=0.999 4), and 2.816-84.48 μg·mL-1 (r=0.999 2), respectively. The average recoveries (RSD) of the five compounds were 98.97%(1.03%), 99.90%(0.92%), 99.89% (1.75%), 99.55% (0.98%), and 99.76%(1.19%) (n=6), respectively. CONCLUSION: The developed method is accurate and precise, which can be used for the quality control of different parts of Dracocephalum moldevica L.The RESULTS: of content determination indicate that the five compounds exist in all the parts of Dracocephalum moldevica L., but the mass fractions are obviously different.