ABSTRACT
Objective: To study the chemical constituents of Chinese eaglewood. Methods: The column chromatography on silica gel, Sephadex LH-20, and semi-preparative HPLC were used to separate and purify the compounds, their structures were elucidated by NMR and MS analyses. Results: Four compounds were isolated from the petroleum ether of Chinese eaglewood and identified as 6-methoxy-7-hydroxy-2-(2-phenylethyl) chromone (1), 6,7-dimethoxy-2-(2-phenylethyl) chromone (2), 6,7-dimethoxy-2-[2-(4'- methoxyphenyl) ethyl] chromone (3), and 6-methoxy-2-[2-(3'-methoxy-4'-hydroxyphenyl) ethyl] chromone (4). Conclusion: Compound 1 is a new compound named as Aquilarone J, and compound 4 is isolated from Chinese eaglewood for the first time.
ABSTRACT
Objective: To investigate the chemical constituents from the leaves of wild Aquilaria sinensis. Methods: The powder of the dried leaves was percolated with 70% acetone. Various chromatographic methods were employed to isolate the compounds and their structures were established by spectroscopic analysis. Results: Thirteen compounds were isolated and identified as 6-E-octadecenoic acid (1), ethyl linoleate (2), apiolin-7, 4'-dimethyl ether (3), 4-cyanobenzaldehyde (4), luteolin-7, 3', 4'-trimethyl ether (5), genkwanin (6), p-phthalic acid di (4-octyl) ester (7), 6-hydroxy-7, 4'-dimethoxyflavone (8), 5-hydroxy-7, 2', 4', 5'- tetramethoxyflavone (9), 5, 4'-dihydroxy-7, 3'-dimethoxyflavone (10), quercetin (11), kaempferol (12), and 4-hydroxybenzoic acid (13). Conclusion: Thirteen compounds have been identified from wild A. sinensis leaves. Compounds 4, 8, and 9 are firstly isolated from the plants of Thymelaeaceae.
ABSTRACT
Objective: To analyze the expression of terpene synthases of Aquilaria sinensis, an endangered south medicine, and to predict the influences of the environmental factors and stresses on the synthesis of terpene in A. sinensis. Methods: Two-year old seedlings and calli were treated by different stresses. The gene expression patterns of terpene synthases were analyzed by real-time qPCR. Results: Wounding and cauterizing could induce the transcriptional expression of terpene synthases in the stems, and the effect of cauterizing was more significant. Low temperature inhibited the transcription of terpene synthases. In calli, methyl jasmonate (MeJA) treatment had the most effective induction. Conclusion: Both wounding and cauterizing could induce the synthesis of terpenes in stems, while the cauterizing treatment might have better effect. Low temperature has negative influence on agarwood formation. For calli, different treatments could induce the terpene synthesis, while MeJA has the best efficacy.