ABSTRACT
Objective: To investigate the chemical constituents from the pericarps of Aquilaria yunnanensis. Methods: The chemical constituents were separated and purified by silica gel, Sephadex LH-20 column chromatography, and semi-preparative HPLC. The structures of isolated compounds were identified by physicochemical properties and spectroscopic data. Results: Thirteen compounds were isolated from the ethyl acetate layer of 95% EtOH extract of the pericarps of A. yunnanensis, and identified as trans-linalool-3,6-oxide-7-O-β-D-(6'-O-acetyl)-glucoside (1), phenethyl-8-O-β-D-(6'-O-acetyl)-glucoside (2), mangiferin (3), iriflophenone-3,5-C-β-D-diglucoside (4), kaempferol-3-O-β-D-glucoside (5), luteolin-7-O-β-D-glucoside (6), isorhamnetin-3-O-β-D- glucoside (7), kaempferol-3-O-β-D-(6″-p-coumaroyl)-β-D-glucoside (8), geraniol-1-O-β-D-glucoside (9), 3-[2-formyl-5- (hydroxymethyl)-1H-pyrrol-1-yl] pentanedioic acid (10), cannabisin D (11), icariside D2 (12), and coniferin (13). Conclusion: Compound 1 is a new compound and compound 2 is a new natural product. Compounds 7, 9-13 were obtained from the Aquilaria genus for the first time. All compounds were firstly isolated from A. yunnanensis.
ABSTRACT
Objective: To study rDNA ITS (internal transcribed spacers) sequences variation from di erent population of Aquilaria sinensis in main habitat of China. Methods: The rDNA ITS regions of various A. sinensis were ampli ed by PCR method and sequenced, and they were analyzed by means of the software of CLUSTAL and MEGA. Results: The sequences of rDNA ITS region of A. sinensis were reported for the rst time, and the sequences of ITS region were 680bp (ITS1 246bp, 5.8S 163bp, ITS2 271bp). There were 6 variable sites among populations and the genetic distance were 0.0% to 1.1%,which indicated the intraspecific genetic variation was low of A. sinensis. Conclusion: The variation of rDNA ITS sequences can be used to authenticate A.sinensis from di erent geographical regions and their adulterants.