All Persistent Pupillary Membranes need not be treated - A case report

Persistent Pupillary Membrane is a common congenital anomaly which appears as a dense network of tissue across the pupil. It is usually asymptomatic, although blurring of vision can be seen in extensive conditions. This is a hospital based case study of an eleven year old girl with bilateral Persistent Pupillary Membrane since birth. The aim of the study is to establish that the treatment approach varies specific to the individual case and when asymptomatic, it can be safely left untreated as in the present study. Surgical Membranectomy, Pupilloplasty, Argon Laser Photocoagulation are the treatment options that can be considered in case of symptomatic conditions.

Effect of fundus retinal argon laser photocoagulation combined with calcium dobesilate in the treatment of diabetic retinopathy / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To explore the clinical value of fundus retinal argon laser photocoagulation combined with calcium dobesilate in the treatment of diabetic retinopathy(DR).<p>METHODS:A prospective study from August 2017 to August 2019 was conducted. Totally 182 patients(326 eyes)with DR were divided into two groups. The observation group received fundus retinal argon laser photocoagulation combined with calcium dobesilate, and the control group received fundus retinal argon laser photocoagulation. Flash electroretinography was performed before treatment and after treatment, and changes in a and b waves were recorded. Changes in the best corrected visual acuity(BCVA), retinal neovascularization fluorescein leakage area, central macular thickness(CMT)and retinal microcirculation parameters were detected. The absorption time of retinal edema, bleeding and exudation was recorded, and the incidence of postoperative complications was counted.<p>RESULTS: At 6mo after treatment, a-wave and b-wave amplitudes of the two groups were decreased(<i>P</i><0.001). The b-wave amplitude of observation group was larger than that of the control group(<i>P</i><0.05). There were no statistically significant differences in a-wave and b-wave peak time and amplitudes between the two groups(<i>P</i>>0.05). The BCVA of the two groups was increased, and the macular leakage area and CMT were reduced(<i>P</i><0.001). Compared with the control group, the BCVA of the observation group was higher, the macular leakage area and CMT were smaller, peak blood flow velocity and average blood flow velocity of the central retinal artery were higher, and the vascular resistance index and pulsatility index were lower(<i>P</i><0.001). The absorption time of edema, bleeding and exudation was shorter in observation group than in the control group(<i>P</i><0.001). There was no significant difference in the incidence of surgical complications between the two groups(<i>P</i>>0.05).<p>CONCLUSION: Fundus retinal argon laser photocoagulation combined with calcium dobesilate is superior to retinal photocoagulation alone in the treatment of DR. The former can promote the improvement of visual acuity, reduce fluorescein leakage, and promote the absorption of retinal edema, bleeding and exudation, which is conductive to improving retinal microcirculation and inhibiting neovascularization.

Subconjunctival bevacizumab and argon laser photocoagulation for preexisting neovascularization following deep lamellar anterior keratoplasty

We report a rare case of deep anterior lamellar keratoplasty (DALK) neovascularization managed with combination of subconjunctival bevacizumab and argon laser photocoagulation. A 24 year old male underwent Deep anterior lamellar keratoplasty for corneal stromal opacity following presumed viral keratitis. Deep corneal neovascularization was observed postoperatively which was successfully managed using a combination of subconjunctival bevacizumab and argon laser photocoagulation within one week of DALK. The neovascularization resolved by 3 months and at 2 years follow up, patient maintained good visual acuity of 6/12 Snellen's without recurrence of vascularization. A combination of bevacizumab and argon laser may be an effective approach to manage neovascularisation in the immediate postoperative phase (Post DALK) and improve graft survival.

Two Cases of Intraocular Lens Pupillary Optic Capture Treated with Argon Laser Iridotomy

PURPOSE: We report two cases of intraocular lens (IOL) pupillary optic capture following IOL scleral fixation treated with argon laser iridotomy. CASE SUMMARY: (Case 1) A 69-year-old man presented with suddenly decreased visual acuity of the left eye. The best-corrected visual acuity was finger count (FC) 30 cm in the left eye. Slit lamp examination revealed subluxation of the left eye IOL. An IOL scleral fixation and anterior vitrectomy were performed and no specific findings were observed. During the follow-up period, pupillary optic capture was repeated without specific causes, such as trauma, or IOL decentration thus, argon laser iridotomy was performed. (Case 2) A 77-year-old man presented with a 3-day history of congestion of the left eye and decreased visual acuity. Best-corrected visual acuity was 0.4 logarithm of the minimum angle of resolution (logMAR) in the left eye. Slit lamp examination revealed subluxation of the left eye IOL. An IOL scleral fixation and anterior vitrectomy were performed, and no specific findings were observed. During the follow-up period, pupillary optic capture was found, and IOL repositioning and argon laser iridotomy were performed. CONCLUSIONS: Laser iridotomy can be used to prevent recurrence of pupillary optic capture in eyes with IOL scleral fixation without decentration or distortion.

Treatment of macular edema secondary to branch retinal vein occlusion with Yiqihuoxuehuayu decoction combined with triamcinolone acetonide and 532nm argon laser / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To observe the clinical value of Yiqihuoxuehuayu decoction combined with triamcinolone acetonide and 532nm argon laser in the treatment of macular edema(ME)secondary to branch retinal vein occlusion(BRVO).<p>METHODS: Totally 80 patients(80 eyes)with ME secondary to BRVO admitted to the hospital from January 2014 to August 2016 were randomly divided into control group and observation group, with 40 cases(40 eyes)in each group. Control group was given intravitreal injection of triamcinolone acetonide combined with 532nm argon laser photocoagulation, and observation group was additionally given Yiqihuoxuehuayu decoction. The best corrected visual acuity(BCVA), central foveal thickness(CMT)and intraocular pressure were measured before treatment and at 1wk, 1mo and 3mo of treatment. The visual acuity, improvement effects of ME and improvements of symptoms(metamorphopsia, hemorrhage, exudation, <i>etc.</i>)were compared between the two groups, and the incidence rate of complications and the recurrence rate at 3mo after treatment were statistically analyzed.<p>RESULTS: There were significant differences in the BCVA and CMT between the two groups at different time points of treatment(<i>P</i><0.05), and the BCVA in observation group at different time points after treatment was higher than that in control group while the CMT was lower than that in control group(<i>P</i><0.05). There was no significant difference in intraocular pressure between the two groups at different time points of treatment(<i>P</i>>0.05). The visual acuity and ME improvement effects in observation group were better than those in control group, and the metamorphopsia and hemorrhage exudation absorption were better than those in control group(<i>P</i><0.05). There were no significant difference in the incidence rate of complications and the recurrence rate at 3mo after treatment between the two groups(<i>P</i>>0.05).<p>CONCLUSION: Yiqihuoxuehuayu decoction combined with triamcinolone acetonide and 532nm argon laser has definite efficacy in the treatment of ME secondary to BRVO, and it can improve visual acuity, reduce macular edema and promote fundus hemorrhage exudation absorption, and it has high safety.

Clinical Observation of Intravitreal Injection of Ranibizumab Combined with Argon Laser Therapy in the Treatment of Ischemic Neovascular Glaucoma / 中国药房

OBJECTIVE:To observe the clinical efficacy and safety of intravitreal injection of ranibizumab combined with argon laser therapy in the treatment of ischemic neovascular glaucoma. METHODS:A total of 166 patients with neovascular glaucoma selected from our hospital during Mar. 2014-Mar. 2016 were divided into control group and observation group according to random number table,with 88 cases in each group(there were 117 and 125 eyes respectively). Both groups received argon laser photocoagulation therapy twice a week for 1 month. Observation group was additionally given intravitreal injection of Ranibizumab injection(0.5 mg)once a week,for 1 month. Clinical efficacies of 2 groups were observed,and the intraocular pressure and visual acuity of 2 groups were observed before treatment,1 week and 1 month after treatment. The occurrence of ADR was recorded. RESULTS:After 1 month of treatment,total response rate was 95.20％ in observation group,which was significantly higher than 76.92％ in control group,with statistical significance (P<0.05). After 1 week of treatment,intraocular pressures of observation group were lower than before treatment and control group at the same time,while visual acuity was better than before treatment and control group at the same time,with statistical significance (P<0.05). After 1 month of treatment,intraocular pressures of 2 groups were decreased significantly and visual acuity was significantly improved;intraocular pressure was decreased and visual acuity was improved more significantly in observation group than control group,with statistical significance(P<0.05 or P<0.01). The incidences of ADR of 2 groups were 3.41％ and 2.27％,without statistical significance (P>0.05). CONCLUSIONS:The ischemic intravitreal injection of ranibizumab combined with argon laser therapy is better than argon laser therapy alone in the treatment of ischemic neovascular glaucoma,and can significantly reduce intraocular pressure and improve visual acuity with good safety.

Effect of Mingjing decoction with argon laser on plasma VEGF in patients with diabetic retinopathy / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To investigate the effect and mechanism of Mingjing decoction combining with argon laser on the levels of plasma vascular endothelial growth factor (VEGF) in patients with diabetic retinopathy(DR). METHODS:The 66 patients(118 eyes)with DR at Ⅲ ~ Ⅴstage were randomly divided into two groups (treatment group and control group). The control group (33 patients with 56 eyes ) was treated with argon laser, and the treatment group (33 patients with 62 eyes) was treated with mingjing decoction combining with argon laser, and a blank group(30 eyes) was set up. The levels of plasma VEGF were detected by enzyme linked immunosorbent assay (ELISA). The results of best corrected visual acuity and fundus fluorescein angiography(FFA) were analyzed, as well as detected glycosylated hemoglobin ( HbA1c), blood coagulation function and the function of liver and kidney. RESULTS: Patients with DR had significantly risen plasma VEGF before treatment when compared with blank control group ( P < 0. 05 ). After 3mo of combined treatment, the levels of plasma VEGF significantly reduced and HbA1c decreased in patients treated with mingjing decoction and argon laser, there were statistically significant difference compared to control group ( P < 0. 05 ). In the treatment group, the best corrected visual acuity and FFA were significantly improved in patients treated with mingjing decoction combining with argon laser compared to patients treated with argon laser alone after 3mo of combined treatment. CONCLUSION: Mingjing decoction combining with argon laser for DR can effectively reduce the level of plasma VEGF, stabilize blood sugar levels, improve the function of retina, and delay DR progresses.

Bilateral foveal retinoschisis accompanying unilateral peripheral retinoschisis.

X-linked juvenile retinoschisis is a rare hereditary retinal disease characterized by a tangential splitting of the neurosensory retina which may cause early-onset visual impairment. Existence of the retinal neurosensory layer splitting on cross-sectional images of optical coherance tomography (OCT) and the absence of leakage on fluorescein angiography (FA) help confirming the diagnosis. Such diagnostic tests are also helpful in determining the management of the disease. However, most of the retinoschisis cavities remain stable and rarely extend to the posterior pole, many authors suggest laser prophylaxis to avoid the potential risk of retinal detachment due to holes in the outer retinal layer. Herein, we report a case with bilateral foveal retinoschisis accompanying unilateral peripheral retinoschisis who was evaluated with detailed ophthalmologic examination. Visual acuity, fundoscopy, OCT, and FA remained stable in the second year of follow-up after prophylactic argon laser treatment.

Effects of Argon Laser Iridotomy on the Corneal Endothelium of Pigmented Rabbit Eyes

PURPOSE: In Asian countries, laser iridotomy for the treatment of angle-closure glaucoma is a common cause of bullous keratopathy, which may be associated with a shallow anterior chamber and dark iris pigmentation in Asians. Several cases of corneal decompensation after argon laser iridotomy have been reported. In the present study, we evaluated the harmful effects of argon laser iridotomy on the corneal endothelium. METHODS: Argon laser iridotomy was performed on the right eyes of pigmented rabbits. Changes in corneal thickness and endothelial cell density after laser iridotomy were evaluated. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was performed for assessment of corneal endothelial cell apoptosis. Combined staining with alizarin red and trypan blue, as well as a live/dead cell assay, were performed for evaluation of damage to the corneal endothelium induced by laser iridotomy. RESULTS: Corneal thickness did not change immediately after laser iridotomy; however, a significant increase was observed 24 hours after iridotomy (p = 0.001). The endothelial cell density of laser-treated eyes four days after laser iridotomy was significantly decreased compared with control eyes (p < 0.001). TUNEL staining showed many TUNEL-positive cells in the corneal endothelium and corneal stroma. No endothelial trypan blue-stained cell nuclei were observed after laser iridotomy; however, several large endothelial cells with damaged membrane integrity were observed. The live/dead cell assay clearly showed a large number of dead cells stained red in several areas throughout the entire corneal button 24 hours after iridotomy. CONCLUSIONS: Argon laser iridotomy induces corneal endothelial cell apoptosis in pigmented rabbit eyes, resulting in decreased endothelial cell density.

Effects of Argon Laser Iridotomy on the Corneal Endothelium of Pigmented Rabbit Eyes

PURPOSE: In Asian countries, laser iridotomy for the treatment of angle-closure glaucoma is a common cause of bullous keratopathy, which may be associated with a shallow anterior chamber and dark iris pigmentation in Asians. Several cases of corneal decompensation after argon laser iridotomy have been reported. In the present study, we evaluated the harmful effects of argon laser iridotomy on the corneal endothelium. METHODS: Argon laser iridotomy was performed on the right eyes of pigmented rabbits. Changes in corneal thickness and endothelial cell density after laser iridotomy were evaluated. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was performed for assessment of corneal endothelial cell apoptosis. Combined staining with alizarin red and trypan blue, as well as a live/dead cell assay, were performed for evaluation of damage to the corneal endothelium induced by laser iridotomy. RESULTS: Corneal thickness did not change immediately after laser iridotomy; however, a significant increase was observed 24 hours after iridotomy (p = 0.001). The endothelial cell density of laser-treated eyes four days after laser iridotomy was significantly decreased compared with control eyes (p < 0.001). TUNEL staining showed many TUNEL-positive cells in the corneal endothelium and corneal stroma. No endothelial trypan blue-stained cell nuclei were observed after laser iridotomy; however, several large endothelial cells with damaged membrane integrity were observed. The live/dead cell assay clearly showed a large number of dead cells stained red in several areas throughout the entire corneal button 24 hours after iridotomy. CONCLUSIONS: Argon laser iridotomy induces corneal endothelial cell apoptosis in pigmented rabbit eyes, resulting in decreased endothelial cell density.

Researching progress in selective laser trabeculoplasty for glaucoma / 中华实验眼科杂志

Glaucoma is the second leading cause of irreversible blindness worldwide.The treatment of glaucomatous eyes is a long-term procedure.Laser treatment is becoming one of the three major methods to lower intraocular pressure (IOP) of glaucomatous patients.Selective laser trabeculoplasty (SLT) mainly targets to the pigment cells on the trabecular meshwork and makes it easier for fluid to flow out of the front part of the eye,decreasing pressure in the eye.But,the actual mechanism of this surgery is below understood now.Compared with other laser therapy,SLT uses a lower-level laser to open the drainage angle of the eye,and therefore cause rare or slight complication,so it is thought to be a repeatable therapy to the patients who needs further treatment.The principle,clinical application,efficacy,safety and study progress about SLT for glaucomatous eyes are reviewed.

Effect of Argon Laser Curing on the Shear Bond Strength of Composite Resin Restorative Material.

Aim: The aim of this in vitro study was to analyze the shear bond strength of composite resin using two different curing light sources: halogen light (control group) for 20 or 40s, and Argon laser (test group) for 10 or 15s. Study Design: This study was carried in the Department of Dental Biomaterials, Faculty of Dentistry and Ophalmolgy Hospital, Mansura University between January 2011 and August 2012. Methodology: Sixty freshly extracted human molars were prepared to receive composite resin samples in four groups "n=15". The teeth were centrally horizontally mounted in plastic molds with cold cure acrylic resin. Flat occlusal surfaces were prepared and smoothed. One-step self-etching dental adhesive (Xeno®111) was applied to the dentine surface and cured. Composite resin (Spectrum universal composite) was inserted into standardized Perspex mold and polymerized with a halogen light and an argon laser curing units. Specimens were stored in deionized water at 37oC for 48 hours. The specimens were stressed under shear force at a crosshead speed of 0.5 mm/min using Lloyd testing machine. The shear bond strength was calculated to the four groups and statistical analysis performed (One-way ANOVA and LSD tests) at the level of significance at p<0.05. Results: LSD tests indicated that there was significantly higher shear bond strength for the composite cured with argon laser compared with the halogen light curing method. There was no statistical significant difference between curing times of the same light source. Conclusions: The argon laser is a promising source for optimal initiation of polymerization of composite resins. The use of argon laser has been suggested as a new alternative for polymerization of restorative materials. The shear bond strength of composite resin cured with argon laser was superior to that was cured with halogen light even with short curing times.

Fotocoagulación con láser argón en el síndrome de transfusión feto-fetal

Se comunica el primer caso de cirugía fetal a nivel nacional en una gestante primigesta de 27 semanas con el diagnóstico de síndrome de transfusión feto-fetal, con categoría Quintero III, realizado en el Hospital Nacional EsSalud Huancayo Ramiro Priale Priale, utilizándose la técnica de fotocoagulación con láser de argón, por vía transcutánea. El resultado postoperatorio fue exitoso, con sobrevida en ambos fetos, los cuales nacieron vía cesárea luego de 25 días de la fotocoagulación, por presentar rotura prematura de membranas.

The first case of fetal surgery in Peru in a woman in her first 27 weeks pregnancy and diagnosis of category Quintero III feto-fetal transfusion syndrome is reported. It was performed at Hospital Nacional EsSalud Huancayo Ramiro Priale Priale using transcutaneous argon laser photocoagulation. Post operatory outcome was good and fetuses were born by cesarean section after 25 days from photocoagulation due to premature rupture of membranes.

Análise química e morfológica do esmalte dentário humano tratado com laser argônio durante a colagem ortodôntica / Chemical and morphological analysis of the human dental enamel treated with argon laser during orthodontic bonding

INTRODUÇÃO: as principais utilizações do laser argônio na Ortodontia são a redução do tempo de polimerização durante a colagem ortodôntica e o aumento da resistência à cárie do esmalte dentário. OBJETIVO: o objetivo deste trabalho foi avaliar as alterações químicas e morfológicas do esmalte dentário humano tratado com laser argônio nos parâmetros da colagem ortodôntica. MÉTODOS: quinze primeiros pré-molares hígidos, extraídos por indicação ortodôntica, foram selecionados e seccionados no sentido do longo eixo em dois segmentos iguais. Uma metade de cada elemento dentário foi tratada e a outra permaneceu sem tratamento. Um total de 30 amostras foi analisado, criando o grupo laser (n = 15) e o grupo controle (n = 15). O tratamento foi feito com laser argônio com 250mW de potência por 5 segundos, com densidade de energia de 8J/cm². RESULTADOS: a análise de difração de raios X demonstrou duas fases em ambos os grupos, as fases apatita e monetita. A redução da fase monetita foi significativa após o tratamento com laser, sugerindo maior cristalinidade. A análise de Espectroscopia de Energia Dispersiva (EDS) indicou aumento na razão cálcio-fósforo no grupo laser, compatível com a diminuição da fase monetita. A morfologia superficial do esmalte dentário apresentou-se mais lisa após o tratamento com laser argônio. CONCLUSÕES: o aumento de cristalinidade e a lisura superficial do esmalte no grupo laser são fatores sugestivos de aumento de resistência à cárie no esmalte dentário.

INTRODUCTION: The main utilities of the argon laser in orthodontics are the high speed curing process in orthodontic bonding and the caries resistance promotion of the tooth enamel. OBJECTIVE: The objective of this study was to evaluate the chemical and morphological changes in the tooth enamel treated with the argon laser in the orthodontic bonding parameters. METHODS: Fifteen sound human first premolars, removed for orthodontic reason, were selected and sectioned across the long axis in two equal segments. One section of each tooth was treated and the other remained untreated. A total of thirty samples was analyzed, creating the laser group (n =15) and the control group (n =15). The treatment was done with 250 mW argon laser beam for 5 seconds, with energy density of 8 J/cm². RESULTS: The X-ray analysis demonstrated two different phases in both groups, the apatite and the monetite phases. The reduction of the monetite phase was significant following laser treatment, suggesting higher crystallinity. The EDS analysis showed an increase in the calcium-phosphorus ratio in the laser group, linked with the decrease of the monetite phase. CONCLUSIONS: The surface morphology was smoother after the laser exposure. The results of high crystallinity and superficial enamel smoothness in the laser group are suggestive of the caries resistance increase of the tooth enamel.

Lasers in primary open angle glaucoma.

Lasers have been used in the treatment of primary open angle glaucoma (POAG) over the years, with the hope that they would eventually replace medical and surgical therapy. Laser trabeculoplasty (LT) is an application of argon, diode, or selective laser energy to the surface of the trabecular meshwork to increase the aqueous outflow. The mechanisms by which intraocular pressure (IOP) is lowered could be mechanical, biologic, or by division of adjacent cells. It is commonly used as an adjunct to medical therapy, but is contraindicated if the angle is obstructed, e.g., peripheral anterior synechia (PAS) or developmental glaucomas. About 75% of individuals will show a significant fall in IOP after argon laser trabeculoplasty (ALT), and the response is similar with selective laser trabeculoplasty (SLT). The effects of LT are not always long lasting, with about 10% of individuals showing a rise in IOP with every passing year. Laser thermal sclerostomy, ab interno or externo, is an alternative to other full-thickness filtration procedures. Longer wavelengths in the infrared range have water-absorptive characteristics that facilitate perforation of the sclera. These lasers can be used to avoid intraocular instrumentation and minimize conjunctival trauma.

A Stereo Microscopic Evaluation of Microleakage - Laser Versus Visible Light Cured ClaSS V Composite Resin Restorations - An Invitro Study.

Composite resins are the most commonly used direct restorative materials in present day practice. Argon laser has been approved for photo polymerization of light Sensitive restorative materials. In this study a Comparative evaluation was made to assess the Marginal sealing ability of Visible light cured and argon laser cured class V Composite resin restorations. Occlusal and cervical areas were compared in degree of marginal leakage by giving separate scores for both the areas. Marginal leakage was higher in cervical margin than the occlusal margin in both the groups. A higher degree of marginal leakage was observed in laser cured restorations compared to the visible light cured restoration.

Laser treatment in 341 patients with exudative age-related macular degeneration / 国际眼科杂志(Guoji Yanke Zazhi)

AIM:To document the prognosis of laser treatment in patients with exudative age-related macular degeneration (AMD).METHODS:Efficacy of the intervention was evaluated using a before-after method.RESULTS:A total of 392 eyes of 341 patients with exudative AMD were examined.77.6% had choroideal neovascularisation (CNV).Before the use of indocyanine green (ICG) angiography,occult CNV was detected in only 1.8% of the eyes,but after the use of ICG angiography,this increased to 19.5% (P<0.001).Of the 349 eyes which were followed up,visual acuity had remained stable in 68.2% of the eyes.There was a statistically significant relationship between localization of lesion and visual acuity changes on pre-and post-laser treatment (P<0.001).Also there was a statistically significant relationship between localization of lesion and recurrence (P<0.05).The recurrence was less in subfoveal lesions than that in juxtafoveal and extrafoveal lesions.CONCLUSION:ICG angiography is highly important in the treatment of occult CNV.

Change in Angle Parameters Measured by Anterior Segment Optical Coherence Tomography after Laser Peripheral Iridotomy Alone versus Laser Peripheral Iridotomy and Argon Laser Peripheral Iridoplasty

PURPOSE: To compare the changes in angle parameters after laser peripheral iridotomy (LPI) alone versus LPI and ALPI (argon laser peripheral iridoplasty) in primary angle closure patients by using anterior segment optical coherence tomography (AS-OCT). METHODS: A total of 25 eyes from 17 patients with narrow angles were enrolled in this present study. Eleven eyes in the LPI treatment group and 14 eyes in the LPI and ALPI combined treatment group were evaluated using AS-OCT. The anterior chamber depth (ACD), angle opening distance at 500 microm (AOD 500) and 750 microm (AOD 750), angle recess area at 500 microm (ARA 500) and 750 microm (ARA 750), trabecular-iris space area at 500 microm (TISA 500) and 750 microm (TISA 750), trabecular-iris angle (TIA) were measured. The pre- and post-treatment parameters were compared in each group. The parameter changes after laser treatment were also compared. RESULTS: AOD 500, AOD 750, ARA 500, ARA 750, TISA 500 and TISA 750 except ACD significantly increased following LPI treatment (p = 0.013, p = 0.010, p = 0.008, p = 0.003, p = 0.006, p = 0.003, p = 0.013, respectively, Wilcoxon signed rank test) and LPI and ALPI combined therapy (p = 0.001, p = 0.001, p = 0.001, p = 0.001, p = 0.001, p = 0.001, p = 0.001, respectively, Wilcoxon signed rank test). The AOD 500 difference, TISA 500 difference, and TISA 750 difference were significantly greater after LPI and ALPI combined therapy than after LPI treatment alone (p = 0.112, p = 0.147, p = 0.049, p = 0.037, respectively, Mann-Whitney U-test). CONCLUSIONS: The results from the present study showed LPI alone or LPI and ALPI combined therapy significantly widened the anterior chamber angle and combined therapy showed greater effect than LPI alone.

Inhibitory effect of recombinant human endostatin on choroidal neovascularization in a rabbit model / 中华实验眼科杂志

Background Choroidal neovascularization (CNV) is a serious complication of many fundus diseases.A variety of factors are associated with CNV.Research showed that recombinant human endostatin ( rhendostatin ) can arrest the vascular endothelial growth factor (VEGF) pathway and inhibit the proliferation of endothelial cells and angiogenesis.Objective This study was to observe the inhibition of rh-endostatin on experimental CNV.Methods The CNV animal models were created by Argon laser with the wavelength 532 nm to irradiate the inferior retina away optical disc 1-2 DD for 25 spots in 32 eyes of 16 chinchilla rabbits.The laser parameters were as follows:power 800 mW,spot diameter 75 μm and time shutter 50 ms.The models were then divided into model control group and rh-endostatin group.Rh-endostatin was intravitreously injected via scleral incision in 16 eyes of 8 model rabbits at 1 week after photocoagulation.Fundus fluorescein angiography (FFA) and optical coherence topography(OCT) were performed at 1,2,4 weeks after photocoagulation respectively.The eyeballs were enucleated and the retinal sections were prepared for the histopathologieal examination,and the contents of VEGF and pigment epithelial derived factor(PEDF) in rabbit vitreous,and blood serum were detected by ELISA at 2,4 weeks after photocoagulation.Results Retinal edema and exudes were seen in 1 week and scarring in 4 weeks after photocoagulation.In rh-endostatin injection group,the hyperfluorescence masses were seen in the background phase and early arterial phase in 42％ (84/200) of spots in the first week.The fluorescence leakage was decreased in the rh-endostatin injection group compared with control group in the second week and ceased at the third week on the FFA after injection.Variety forms of hyperreflective zones were found below the retinal pigment epithelium on the seventh day after photocoagulation.But the partial vessel occlusion and fibroplasias were identified in the rh-endostatin injection group in the third week by the OCT.The histopathological examination showed that the morphological abnormality was mild in the rh-endostatin injection group in comparison with model group.The serum PEDF concentration was significantly elevated but the VEGF/PEDF values in vitreous and serum were declined in rhendostatin injection group compared with model group (P ＜ 0.0 1 ).Conclusions Argon laser photocoagulation could induce the experimental CNV in chinchilla rabbit.Intravitreous injection of rh-endostar can effectively inhibit laser-induced CNV in rabbit.

Clinical Features of Conjunctival Nevi in Korean Patients

PURPOSE: To evaluate clinical features and therapeutic modality of conjunctival nevi in Korean patients. METHODS: A retrospective analysis was performed on 197 patients (75 males and 122 females) with nevi who were diagnosed by slit lamp examination from 1997 to 2008. RESULTS: Nevi occurred most commonly on bulbar conjunctiva (88%), followed by caruncle and plica semilunaris (7%). The nevi involved temporal (71%), nasal (21%), inferior (2.8%) and superior (0.7%) quadrants of the conjunctiva. The mean horizontal length was 4.3+/-2.0 mm and the mean vertical 4.45+/-2.2 mm. Thirty-five patients (7.8%) received no treatment. Excisional biopsy was performed in 38 patients (19.3%). Argon laser photoablation of conjunctiva nevi was performed in 124 patients (62.9%). CONCLUSIONS: The pattern of conjunctival nevi in Korean patients was similar to Caucasian patients. The biopsy was performed according to appropriate guidelines however, nevus which does not require a biopsy, could be treated by argon laser photoablation.