ABSTRACT
Objective:To establish a gas chromatography(GC) fingerprints analysis method of farmed musk,natural musk and artificial musk for effective control and scientific evaluation of the quality of musk. Method:The sample was split in the 250℃ injection port,with 1:1 split ratio,and separated on a Capillary column(325℃,0.25 mm×30 m,0.25 μm). Nitrogen was used as a carrier gas,the flow rate was 1 mL·min-1. The detector temperature was 280℃. The temperature program was 80℃ for 2 min,increase by 5℃·min-1 to 160℃, and by 1℃·min-1 to 200℃ for 20 min,then 5℃·min-1 to 260℃ for 20 min. GC of the 30 batches of farmed musk,24 batches of natural musk and 8 batches of artificial musk were analyzed by adopting temperature programmer. The similarity evaluation system for chromatographic fingerprint of TCM(2004A)was used to evaluate the similarity of these chromatograms. The fingerprint peaks were identified by using GC-MS combination technology. The ion source temperature was set at 230℃. The quadrupole temperature was set at 150℃. The GC-MS interface temperature was set at 280℃. Electron impact(EI)was used as the ionization source,and the electron energy was set at 70 eV. The scanning scope was m/z 30-550. Result:The GC fingerprints of different kind of musk were established,and the similarities all conformed to regulations. Totally 17 common compounds in farmed musk,10 common compounds in natural musk,and 8 common compounds in artificial musk were determined and compared. Conclusion:This method features a high precision,reproducibility,and stability. The established fingerprints could be used for the overall quality evaluation for musk.
ABSTRACT
The rat models of focal cerebral ischemic reperfusion and subarachnoid hemorrhage were used to evaluate the therapeutic effects of artificial musk to provide support for its clinical application. All animal experiments were performed following the regulations of the Animal Ethics Committee of Peking Union Medical College. The results showed that oral administration of artificial musk had significant protective effects on acute ischemic and hemorrhagic stroke. In the dose range of 10-200 mg·kg-1, the mortality, neurobehavioral and cerebral infarction volume of rats in model group indicated a clear dose dependent relationship. The effective dose of artificial musk is 10 mg·kg-1 in ischemic stroke and 200 mg·kg-1 in hemorrhagic stroke. These findings suggest that the treatments of artificial musk in ischemic stroke and in hemorrhagic stroke are different, and such differences should be noted for its clinical use.
ABSTRACT
Objective To establish a quality control method for bomeol and artificial musk in Xinfufang-Zhenzhusan and Xinfufang-Zhenzhugao.Methods We used petroleum ether-toluene-ethyl acetate (9:3:2)as developer for TLC to identify isoborneol and borneol and petroleum ether-dichloromethane (2:3) as developer for TLC to identificate musk ketone.Agilent 7890 B gas chromatograph,FDI detector;Column:Thermo-TG-WaxMS GC (0.25 mm × 30 m,0.25 mm) was employed;the carrier gas was high purity nitrogen and flow rate for 1 mg/ml,the injection port temperature is 200 C and detector temperature is 250 ℃;the split ratio is 10:1 and injection volume was 1 μl,using temperature programmed.Results The isoborneol,borneol and musk ketone in the range of 0.001-10 mg/ml showed good linearity.The recovery of the method is in the range of 95 ％ to 105 ％.The TLC for isobomeol,bomeol,musk ketone can be identified easily.Conclusions The method was simple and reasonable,which can be used for the quality control of borneol and artificial musk in the Xinfufang-Zhenzhusan and Xinfufang-Zhenzhugao.