ABSTRACT
Abstract The essential oil of Chenopodium ambrosioides L., Amaranthaceae, was obtained by steam distillation in a Clevenger apparatus and characterization was performed using chromatographic and spectroscopic assays (GC-FID, GC/MS, 1H NMR). Two major compounds were identified: p-cymene (42.32%) and ascaridole (49.77%). The ethanolic extract and hydrolate were fractionated by liquid–liquid partitioning and the compounds were characterized by GC/MS. The essential oil, ethanol extract and fractions by partitioning with dicloromethane, ethyl acetate and butanol were tested in tumor cell lines (K562, NALM6, B15, and RAJI). Significant cytotoxic activity was found for essential oil (IC50 = 1.0 µg/ml) for RAJI cells and fraction dicloromethane (IC50 = 34.0 µg/ml) and ethanol extract (IC50 = 47.0 µg/ml) for K562 cells. The activity of the essential oil of C. ambrosioides is probably related to the large amount of ascaridol, since the other major compound, p-cymene, is recognized as a potent anti-inflammatory and has low cytotoxic activity.
ABSTRACT
Chenopodium ambrosioides L. é uma erva aromática amplamente distribuída ao longo da América, África e também em países da Europa. No Brasil, a planta é popularmente conhecida como mastruz. Tem sido utilizada pela população como anti-helmíntica e para tratamentos de gripe. O óleo essencial das folhas é o componente mais conhecido da planta e de grande importância científica. As propriedades terapêuticas da planta são geralmente atribuídas ao óleo essencial pelos investigadores. Este trabalho traz uma revisão da literatura sobre o óleo essencial de C. ambrosioides, com ênfase nos seus constituintes químicos, atividade biológica e aspectos toxicológicos.(AU)
Chenopodium ambrosioides L. is an aromatic herb widely distributed throughout the Americas, Africa and also in European countries. In Brazil, the plant is popularly known as mastruz. It has been used by the population as anthelmintic and in the treatment of influenza. The essential oil of the leaves is the most familiar component of the plant and of great scientific importance. The therapeutic properties of the plant are generally attributed to the essential oil. This paper reviews the literature on the essential oil of C. ambrosioides, with emphasis on its chemical constituents, biological activity and toxicological aspects.(AU)
Subject(s)
Oils, Volatile/therapeutic use , Chenopodium ambrosioides , Plants, MedicinalABSTRACT
ObjectiveTo evaluate the gastroprotective activity of ascaridole.MethodsThe gastroprotective effect of ascaridole was evaluated on ulcer healing in rats with acetic acid-induced chronic gastric ulcer,pylorus ligation- and Aspirininduced gastric ulcer.Ascaridole was ig administered with the dosages of 10 and 20 mg/kg once daily for 7 d.Results Ascaridole showed the significant anti-ulcer effects.In acetic acid-induced gastric ulcer rats,the ulcer areas after 10 and 20 mg/kg of ascaridole treatment were (65.1 ± 20.0) and (50.6 ± 11.0) mm2,respectively,which were significant lower (P ＜ 0.01) than that of the control group [(116.7 ± 35.8) mm2].For pylorus ligation model,ascaridole showed a gastric ulcer healing effect in a dose-dependent manner.Ascaridole at the dose of 20 mg/kg showed 50％ ulcer protection and had a significant (P ＜ 0.05) gastroprotective activity since it decreased the total acidity and pepsin activity.Compared to the control group,the two dosages of ascaridole showed the significant reduction (P ＜ 0.05) in the ulcer index on Aspirin-induced ulcer.ConclusionThis study provides evidence that ascaridole shows potential efficacy on the healing of gastric ulcers induced by acetic acid,Aspirin,and pylorus ligation.