Comparison of Three Parasitological Stool Examination Methods with the Formalin-Ethyl Acetate Procedure for the Diagnosis of Intestinal Parasites in Humans

Aims:The objective was to compare the sedimentation spontaneous in tube technique(SSTT), the direct smear, the zinc sulfhate(surface film and sediment analyzed) methods with the formalin-ethyl acetate method as the reference standard.Study Design:This a cross-sectional study performed in two populations aroundChapala lake, Jalisco, Mexico.Place and Duration of Study:Sample Department of Medical Sciences and Life, University Center of the Ciénega, University of Guadalajara, Ocotlán, Jalisco, Mexico. Department of Physiology, Health Sciences University Center. University of Guadalajara, Jalisco. México, between June 2018 andJuly 2019.Methodology:Sample: We included a total of 297 samples and were analyzed by the direct smear, the zinc sulfhate (surface film and sediment analyzed), the SSTT and formalin-ethyl acetate methods.Results:The SSTT was able to detect 40% of intestinal parasites, very good agreement for Entamoebacomplex, Entamoeba coli, andAscarislumbricoideskappa index=0.697, 0.791, 0.696, respectively and excellent agreement for Giardia lambliakappa index = 0.843. Regarding the isolation ofBlastocystisspp, only a poor agreement was found among all techniques. The SSTT was able to detect multiple parasites with a sensitivity of 82.90% and specificity 83.80 kappa index= 0.649.Conclusion:The SSTT showed a very good agreement for the diagnosis intestinal polyparasitism which could represent another alternative for the concentration and identification protozoans and intestinal helminthes in low-resource settings

Life-threatening complications of ascariasis in trauma patients: a review of the literature / 世界急诊医学杂志（英文）

@#BACKGROUND: Ascariasis is one of the most common human parasitic infections worldwide. In some rare cases, ascariasis may cause serious consequences even sudden death. This study was undertaken to review the life-threatening complications of ascariasis in trauma patients reported in the literature. DATA SOURCES: Relevant articles about ascariasis and trauma were searched from Pubmed, Google scholar, Scirus, and Wanfang databases. RESULTS: Twenty-four patients with ascariasis were collected from 21 articles searched. Most of these patients were from tropical and subtropical countries. Of the 24 patients, 12 were children. Their major complications occurred in the airway passage and digestive tract. There were 3 fatal cases in these patients. Twelve of the 24 patients described in 10 articles were reported in the last 10 years. CONCLUSIONS: Early diagnosis and prompt intervention are essential to minimize the high morbidity and mortality of these serious complications in trauma patients. Physicians should be aware of the possibility of Ascaris infection in a trauma patient from endemic area of ascariasis. History of Ascaris infection and routine examination of feces forAscaris eggs may be helpful to make a correct diagnosis.

Hemorragia posparto y ascariasis: presentación de un caso / Postpartum hemorrhage and ascariasis: a case reporte

Resumen La hemorragia posparto es una de las principales complicaciones maternas que genera morbimortalidad. Se asocia diversas causas, como atonía uterina, retención de restos placentarios y coagulopatías. Una poco conocida es la infección por Ascarislumbricoides. Se realiza la revisión de un caso clínico de paciente de 16 años de edad, que evolucionó a histerectomía secundaria a ascariasis y muerte materna, que ingresó al Servicio de Urgencias Obstétricas del Hospital de la Universidad del Norte. Según nuestro conocimiento, es el primer caso descrito en nuestro país en el que se sospecha la asociación de hemorragia posparto y la infección por Ascarislumbricoides.

Abstract Postpartum hemorrhage is one of the most important obstetrical complications, having a high morbidity and mortality rate. It is associated to different causes such as uterine atony, placental birth alterations, and coagulopathy. One not so well-known cause is the infestation with the parasite Ascarislumbricoides. We report a case of a 16-year-old patient who required an emergency postpartum hysterectomy until progressing to death due to ascariasis. To our current knowledge, this is the first reported case in Colombia where there is a suspected association between the infestation with Ascarislumbricoides and postpartum hemorrhage.