ABSTRACT
Objective To investigate the effect of the asthmatic mice's airway inflammation and bronchial hyperresponsiveness treated with curcumin.Methods The mice were divided into three teams randomly,the normal mice,the asthmatic mice and the curcumin mice.The mice of three teams were detected by lung function,Giemsa dying,HE and PAS dying,and ELISA.Results After the Mch concentration of 6.25 g/L,the value of Penh in asthmatic mice was higher than the control mice,which was sighifiantly different(P ＜ 0.01).However,the value of Penh in curcumin team was lower than asthmatic mice,which was sighifiantly different (P ＜0.01).The number of total white blood cells and eosinophils was higher in asthmatic mice than the contol,which was sighifiantly different(P ＜0.01).However,the number in curcumin team was lowered than asthmatic team(P ＜ 0.01).The IgE content of BALF in asthmatic mice was higher than the control,which was significantly different(P ＜ 0.01).However,the content in curcumin team was lowered than the asthmatic mice,which had a significant difference(P ＜0.01).Pathology of HE staining in asthmatic mice showed the thickening bronchial wall,narrow lumen,peribronchial and perivascular infiltration with a large number of eosinophil-based inflammatory cells,lumen with many inflammatory secretions.However,the curcumin team was alleviated than the asthmatic mice.There were more goblet cells and more mucus secretion in the asthmatic mice by PAS staining.However,the curcumin team was alleviated than the asthmatic mice.Conclusion Curcumin can alleviate the airway inflammation,mucus secretion,airway hyperresponsiveness and the IgE content of bronchoalveolar lavege fluid.
ABSTRACT
Objective To investigate the effect of the asthmatic mice's contents of nuclear factorerythroid-2-related factor 2,Nrf2 and HO-1 reated with curcumin in lung tissue.Methods All 45 mice were divided into three teams randomly,the normal mice,the asthmatic mice and the curcumin mice group.We tested the expression of Nrf2 and HO-1 of three mice teams in lung by immunolfluorescence technique.We tested the protein contents of Nrf2 and HO-1 of the three teams in the lung tissue by Western blot.We tested the binding activity of Nrff2 and ARE of the three teams in lung by EMSA.Results The expression of Nrf2 and HO-1 in curcumin group was significantly higher than that in asthma group and normal control group by immunolfluorescence technique.There was no sigrnificant difference in content of cytoplasm Nrf2 protein in lung tissue between three groups by western blot(P ＞ 0.05).The content of nuclear Nrf2 and HO-1 protein in the lung tissue of the curcumin group was significantly higher than that in the other two groups,the difference is statistically significant (Nrf2,P ＜0.05;HO-1,P ＜0.01).The binding activity of Nrf2-ARE in lung tissue of curcumin group was significantly higher than that of asthma group and control group,with statistical significance(P ＜ 0.05).Conclusion Curcumin can increase the protein contents of the Nrf2 and HO-1 of mice and enhance the expression of them.
ABSTRACT
Objective To investigate the effect of the asthmatic mice's contents of NF-κB、IκB、p-IκB treated with curcumin.Methods Thirty Balb/c mice were divided into three groups randomly,the normal mice,the asthmatic mice and the curcumin mice.The bronchial hyperresponsiveness of the three groups were exanined by lung function.The NF-κB 、IκB 、p-IκB content of three teams were tested.Results The content of cytoplasm NF-κB in asthmatic mice was lower than the control (P ＜ 0.01).However,the content of cytoplasm NF-κB in curcumin team was more than the asthmatic mice (P ＜ 0.05).In the other hand,The content of nuclear NF-κB in asthmatic mice was more than the control(P ＜0.01).However,the content of nuclear NF-κB in curcumin mice was lower than the asthmatic mice (P ＜ 0.05).The content of cytoplasm p-hκB in asthmatic mice was more than the control and the content of IκB was lower than the control(P ＜ 0.01).Howerer,the content of IκB in curcumin team have much more than the asthmatic mice and the content of p-IκB have much lower than the control(P ＜ 0.01).Condusion Curcumin alleviates the airway hyperresponsiveness of the asthmatic mice through the suppression of NF-κB transcribe to the nuclear via alleviating the phosphorylation of I-κB.
ABSTRACT
Objective To evaluate the effects of andrographolide and budesonide (BUD) on eosinophil (EOS) counts and neutrophilic and the expressions of IL-17,IL-23 in asthmatic mice.Methods BALB/c mice were randomly divided into asthmatic group,andrographolide group,BUD group and control group,8 mice in each group.The mice were sensitized and challenged by ovalbumin(OVA) to establish asthmatic mice model.Two groups of mice were treated with andrographolide and BUD,respectively.Twenty-four hours after the last administration,inflammatory cell count,cell classification in bronchoalveolar lavage fluid (BALF) and lung histological examination were carried out.Wright-Giemsa was used to count total,EOS,neutrophilic cells of BALF.Meanwhile,ELISA was used to detect the concentrations of IL-17 and IL-23 of BALF and peripheral blood.The expressions of IL-17 mRNA and IL-23 mRNA of the lung tissue were detected by quantitive Real-time PCR.Results Total cell and EOS counts in BALF of 2 treatment groups [(111.98 ± 3.28) × 107/L and (64.16 ± 36.75) × 107/L,(131.29 ± 4.89) × 107/L and (82.40 ± 11.48) × 107/L] decreased significantly compared with asthma group[(208.78 ± 18.52) × 107/L and (106.80 ± 13.90) × 107/L] (all P ＜ 0.05).IL-17 protein levels in BALF and peripheral blood were not significantly different between administration groups(P ＞ 0.05).IL-23 protein levels in BALF of BUD treated group[89.92 (113.87-67.79) ng/L]were significantly lower than asthma group [93.30 (173.39-42.95) ng/L] (P ＜ 0.05).IL-23 mRNA expressions in treatment groups[2.14 (8.54-0.22) and 1.18 (4.58-0.25)] were significantly suppressed when compared with asthmatic group[5.08 (10.14-1.42)] (P ＜ 0.05),while IL-17 mRNA expressions had no significant difference between them(P ＞ 0.05).Conclusions Andrographolide and BUD can suppress the infiltration of total inflammatory cells and EOS expression IL-23 mRNA in BALF.They had no significant impact on the neutrophilic cells counts and expression of IL-17 in BALF.