ABSTRACT
Objective To study the chemical constituents of the underground parts of Astragalus tatsienensis var. incanus. Methods The compounds were separated and purified by RP18, Sephadex LH-20 column chromatography, as well as preparative liquid chromatography. The structures of the isolated compounds were determined by means of modern spectroscopic analysis and physicochemical properties. Results Four compounds were isolated from the methanol extract of the underground part of A. tatsienensis var. incanus, and identified as 3β-O-α-L-rhamnopyranosyl (1→2)-β-D-galactopyranosyl (1→2)-β-D-glucuronopyranosyl- 22β-hydroxy-11-oxo-olean-12-ene (1), hyperoside (2), wighteone (3), and 4-hydroxybenzoic acid (4). Conclusion Compounds 2-4 are isolated from this plant for the first time, and compound 1 is a new triterpene glycosides compound, named astratatincoside A.