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Objective@#To evaluate, the method of different packing layer and sealing mode for the packaging of the infectious wastes in order to achieve better autoclave sterilization effect.@*Methods@#In the autoclave cycles P4 and P9, different packing layer (single, double and triple) and sealing mode (slightly folded, autoclave tape, nylon tape closure) were applied to the internal infectious waste sterilization, biological indicators were used to detect the effect of autoclave sterilization of the upper, middle and lower layers, respectively.@*Results@#1) In the autoclave cycle P4, the triple autoclave bags packaging sealing method was based on the slight folding, autoclave tape, and nylon taple, the biological indicator negative rate of the internal infectious waste in the upper layer, the middle layer and the lower layer were respectively in the upper layer 100%, 0%, 0%, in the middle layer 67%, 20%, 0%, and in the lower layer was 0%. The double autoclave bags packaging sealing method was based on the slight folding, autoclave tape, and nylon taple, the biological indicator negative rate of the internal infectious waste in the upper layer, the middle layer and the lower layer were respectively the in the upper layer 100%, 85%, 0%, the middle layer 45%, 0%, 0%, and the lower layer 10%, 0%, 0%. the single autoclave bag packaging sealing method wa based on the slight folding, autoclave tape, and nylon taple, the biological indicator negative rate of the internal infectious waste in the upper, middle and lower layers were respectively the upper layer 100%, 100%, 0%, the middle layer 70%, 45%, 0%, and the lower layer 12.5%, 0%, 0%. (2) In the autoclave cycle P9, the triple autoclave bags packaging sealing method was based on the slight folding, autoclave tape, and nylon taple, the biological indicator negative rate of the internal infectious waste in the upper layer, middle and lower layers were respectively the upper layer all 100%, the middle layer 100%, 100%, 70%, and the lower layer 100%, 100%, 30%. The double autoclave bags packaging sealing method was based on the slight folding, autoclave tape, and nylon taple, the biological indicator negative rate of the internal infectious waste in the upper, middle and lower layers were respectively the upper layer all 100%, the middle layer all 100% and the lower layer is 100%, 100%, and 60%. The single autoclave bag packaging sealing method was based on the slight folding, autoclave tape, and nylon taple, the biological indicator negative rate of the internal infectious waste in the upper, middle and lower layers were all 100%.@*Conclusions@#When autoclave is recommended for sterilization of infectious waste, the best effect was achieved with a single unsealed autoclave bag. If it is necessary to seal, the autoclave bag should not be completely sealed, avoiding incomplete penetration of steam into the interior space, otherwise the infectious waste can not be completely sterilized.
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STATEMENT OF PROBLEM: Resonance frequency analysis is evaluated as the non-invasive and objective method for the evaluation of implant stability and has been increasingly used. It is necessary to evaluate the factors affect the ISQ measurement stability. PURPOSE: This study was performed to evaluate the effect of the autoclave sterilization and reuse of Smartpeg(TM) on ISQ measurement. MATERIAL AND METHODs: SmartPeg(TM) (Integration Diagnostics Ltd., Goteborg, Sweden) of autoclave group (A) was autoclave sterilized 9 times and Smartpeg(TM) of reuse group (R) was reused 9 times. Ten SmartPeg(TM)s were allocated to each group and after each autoclave sterilization and reuse, implant stability quotient (ISQ) values were measured 3 times from the two directions a and b at a right angle. Osstell(TM) mentor (Integration Diagnostics Ltd., Goteborg, Sweden) was used and type 1 (article no. 100353) Smartpeg(TM) was selected according to SmartpegTM reference list. Osstem Implant US II fixture (Osstem Co., Seoul, Korea) in 4.0 mm x 11.5 mm was embedded in the self-curing acrylic resin (Orthojet(R), Lang Dental, U.S.A.). Data was statistically analyzed by one-way ANOVA (alpha= .05) and scheffe test was done where a significant difference exist. Correlation test was also done between ISQ value and the number of autoclave sterilization or reuse. RESULTS: 1. In autoclave group, the means and sd. of ISQ value before autoclave sterilization were 84.97 +/-0.41, 84.93+/-0.74 at direction a and b. There was significant differences between autoclave groups at direction a and b (P=.000). 2. In reuse group, the means and sd. of ISQ value before reuse were 85.40+/-0.62, 85.50+/-0.57 at direction a and b. There was no significant difference between reuse groups at direction a and b (P>.05). 3. There was a weak positive correlation between the number of reuse and ISQ value at direction a and b (gamma=.207 and .246, P<.01). CONCLUSION: Within the limitations of this study, the following CONCLUSIONs were drawn. Till ninth reuse of Smartpeg(TM), the ISQ measurement stability did not be affected. After twice autoclave sterilization of Smartpeg(TM), the ISQ measurement stability was affected.
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Humans , Mentors , Seoul , SterilizationABSTRACT
OBJECTIVE To evaluate the disinfecting effect of electrolyzed-oxiding water(EOW) on dental(instruments) contaminated after clinical operation.METHODS Use K-reamer and high-speed handpiece as objects of test.(K-reamers) were disinfected by EOW and 2% glutaraldehyde for 5min and 10min,respectively.And(handpieces) were disinfected by EOW and autoclave sterilization,respectively.The samples were collected from each(K-reamer) and each handpiece′surface and its water-canal before and after disinfection.The samples were done germiculture.RESULTS Disinfecting K-reamer for 10min,the disinfection rate of two disinfectants(EOW and 2%(glutaraldehyde)) were both 100%.Disinfecting handpiece with EOW(by surface-wiping,immersing and rinsing the water-canal)or autoclaving sterilization,the disinfection rate of EOW and 2%glutaraldehyde were both 100%.(Disinfecting) handpiece with EOW(by surface-wiping,immersing and rinsing the water-canal) or autoclaving(sterilizaion),the bacteria contaminated on handpiece′s surface and in the water-canal could all be killed,while(disinfecting) handpieces with EOW only by surface-wiping,and immersing,in the water-canal there were still a lot of bacteria.CONCLUSIONS The disinfecting effect of EOW is reliable and safe compared with 2%(glutaraldehyde) and autoclave sterilization.
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Objective To research the influence of autoclave sterilization on elastic module and flexural strength of five types of posts.Methods Shidelong carbon,glass and quartz fiber post,tenax glass fiber post and Anthogyr titanium post were chosen,and all the samples were divided into two groups which included five subgroups according to the model of posts,each subgroup including six samples.Experimental groups were autoclave sterilized(50 min at 134℃ and 2.2MPa),While the control groups were not.With three point loading system,elastic module and flexural strength of all the samples were measured.Results were statistically analyzed to find the difference between them.Results There was no statistical difference between Shidelong carbon,glass,quartz fiber post and Tenax glass fiber post in elastic module and flexural strength in both experimental group and control group,while Anthogyr titanium post gained the highest elastic module and flexural strength.Paired-samples t test also showed there was no statistical difference between experimental group and control group in elastic module and flexural strength.Conclusion Fiber posts perfectly meet the need of clinical use on flexural strength.Furthermore,with elastic module similar to dentin and much lower than metal posts,fiber posts will help to protect the remaining tooth structure from fracture.Autoclave sterilization has a little influence on elastic module and flexural strength of posts,which allows practitioners to systematically sterilize the posts in clinic.