ABSTRACT
OBJECTIVE: To develop a liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the determination of B07 in rabbit plasma. METHODS: Analytes were separated from plasma by a combination of alkalinized protein precipitation and liquid-liquid extraction with methyl tert-butyl ether. Chromatographic separation was performed on a Waters Symmetry C18 (4.6 mm×150 mm, 5 μm)column with the mobile phase consisted of acetonitrile (containing 0.2% acetic acid)-40 mmol·L-1 ammonium acetate (containing 0.2% acetic acid) in water (80∶20). A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in the positive ion mode. Quantification was performed using multiple reaction monitoring (MRM) of the transitions m/z 529.3→335.4 and m/z 237.1→194.1 for B07 and carbamazepine (internal standard), respectively. RESULTS: The method showed a good linearity in a concentration range of 0.31-310 ng·mL-1 for B07 was more than 0.99. The intra- and inter-day precision was less than 15% and the absolute recovery was above 75%. CONCLUSION: This method was selective and rapid, sensitive for investigating blood drug concentrations in rabbit.