ABSTRACT
The present study investigated the seroprevalance of Visna Maedi Virus (VMV) and Border Disease Virus (BDV) infections in sheeps in regions in and around Van province, Turkey. Sample materials were taken from 360 sheep sent to slaughterhouses around Van. All serum samples were examined using ELISA for antibodies for Visna Maedi (VMV) and Border Disease (BDV) viruses. Of these, 38 (10.5%) tested positive for Visna Maedi virus antibodies and 163 (45.2%) for Border Disease virus antibodies. Varying numbers of samples were positive for both virus antibodies across the towns of Ercis, Çaldiran, Erçek and Baskale in Van, Agri and Hakkari provinces. Both infections should be eliminated by informing veterinarians and animal owners, identifying and eliminating persistently infected animals from flocks, and conducting appropriate eradication measures. Economic support should be provided for this.(AU)
O presente estudo investigou a seroprevalência de infecções por Visna Maedi Virus (VMV) e Border Disease Virus (BDV) em ovelhas nas redondezas da província de Van, na Turquia. Amostras foram retiradas de 360 ovelhas enviadas a um matadouro próximo de Van. Todas as amostras foram examinadas usando ELISA para anticorpos de visna Maedi (VMW) e Border Disease (BDV). Destes, 38 (10.5%) foram positivos para anticorpos virais de Visna Maedi e 163 (45.2%) para anticorpos virais de Border Disease. Números variados de amostras foram positivos para ambos os anticorpos nos municípios de Ercis, Çaldiran, Erçek e Baskale, nas províncias Van, Agri e Hakkari. Ambas as infecções devem ser eliminadas informando veterinários e proprietários, identificando e eliminando animais persistentemente infectados de rebanhos, e conduzindo medidas apropriadas de erradicação. Suporte financeiro deve ser providenciado para tal.(AU)
Subject(s)
Border disease virus/pathogenicity , Seroepidemiologic Studies , Visna-maedi virus/pathogenicity , Enzyme-Linked Immunosorbent Assay/statistics & numerical dataABSTRACT
In order to prepare the monoclonal antibodies (MAbs) by the recombinant phosphoprotein (p24) of Borne dis ease virus (BDV) as immunogen,the spleen cells of immunized balb/c mice with the recombinant BDV p24 were fused with the myeloma cells SP2/0.The hybridoma cell lines secreting MAbs against p24 were obtained after selected by indirect ELISA and subcloned for 3 times.The MAbs were prepared by intraperitoneal injection in mice,purified by affinity chromatography,identified by western blot and immunofluorescence assay (IFA) for specificity.The purified MAbs against BDV p24 from ascites belonged to IgG1 showed a purity of 98％ and 93％,and a titer of 1 ∶ 81 000,and specific reaction with the BDV p24 restructured and expressed in Oligodendroglia cells (OL).The MAbs against BDV p24,with high specificity and sensitivity,were prepared successfully,which laid a basis for the study of diagnostic reagents and pathogenic mechanism of BDV.
ABSTRACT
In order to prepare the monoclonal antibodies (MAbs) by the recombinant phosphoprotein (p24) of Borne dis ease virus (BDV) as immunogen,the spleen cells of immunized balb/c mice with the recombinant BDV p24 were fused with the myeloma cells SP2/0.The hybridoma cell lines secreting MAbs against p24 were obtained after selected by indirect ELISA and subcloned for 3 times.The MAbs were prepared by intraperitoneal injection in mice,purified by affinity chromatography,identified by western blot and immunofluorescence assay (IFA) for specificity.The purified MAbs against BDV p24 from ascites belonged to IgG1 showed a purity of 98％ and 93％,and a titer of 1 ∶ 81 000,and specific reaction with the BDV p24 restructured and expressed in Oligodendroglia cells (OL).The MAbs against BDV p24,with high specificity and sensitivity,were prepared successfully,which laid a basis for the study of diagnostic reagents and pathogenic mechanism of BDV.
ABSTRACT
Objective To study the correlation with the infectious situation of Borna disease with the multiple sclerosisof Zunyi region. Methods Established method of the specific CIC and an antibody of Borna were used to detect the PBMC of 7cases of patients with multiple sclerosis and 93 cases of control group.Results In the collected 7 cases of PBMC in patients with multiple sclerosis, detected 2 positive samples of the specific CIC and antibody of Borna with a positive rate of 28.57%(2/7).Meanwhile, positive plasma samples were also detected in healthy control group, and the positive rate was 7.53%(7/93),The antibody positive rate of control group was also 5.38%(5/93).But between the two groups, the difference was no statistically significant.Conclusion Results indicate that the possibility of BDV infection is presented in Zunyi regions.BDV infection is not necessarily associated with multiple sclerosis.
ABSTRACT
Objective To investigate the expression and location of Borna disease virus(BDV)nucleoprotein in transfected oligodendrocytes,and then to explore its expression mechanisms in oligodendroglial cell(OL)and their effects on cell proliferation.Methods We used PCR to detect BDVp40 gene fragments in transfected OL,laser confocal microscopy and Western blot method to detect the expression of nuclear protein and its intracellular location in the cell,MTT to detect the influence of cell proliferation by nuclear protein on the OL.Results We had detected the BDVp40 gene fragments in transfected oligodendrocytes.The nucleoprotein can be positioned in the cytoplasm and cell membrane by laser scanning confocal microscope;Western blot results showed that there is nucleoprotein in the constitutive protein,but was not detected in the cytoplasm.MTT tests showed that nucleoprotein expressed in OL can inhibit cell proliferation.Conclusion It is indicated that the transfected OL stablely express of BDV nucleoprotein,and itslocation is in the cell structure of proteins,particularly in the cytoplasm and more richer in cell membrane,that indicating they may play a key role in cell signal transduction or into the cell-mediated viral infection.BDV nucleoprotein inhibit proliferation of OL,which maybe an important mechanism of Borna disease virus persistant infection and produce symptoms.