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Aim To explore the establishment of BN rat animal model and RBL-2H3 cell model of allergic reaction to Yinzhihuang injection. Methods ASA test was performed to compare the symptoms and grades of allergic reactions in BN rats and Hartley guinea pigs, and serum IgE and histamine levels were detected. The amount of histamine released from RBL-2H3 cell supernatant was measured after Yinzhihuang injection affected, and the intracellular Ca
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Background: ShuangHuangLian Injection (SHLI) has induced many serious anaphylactic diseases, becoming a threat to the public health. However, study of the mechanism of the reaction and therapeutic approaches to it have been hindered by the lack of suitable animal models. Objective: We sought to develop a rat model that could mimic the characteristics of SHLI-induced anaphylaxis and the immunological changes that occur in clinical use. Methods: Brown Norway (BN) rats were sensitized twice at an interval of seven days with three doses of SHLI and challenged 14 days after the last administration. Different parameters, including the symptoms, the histamine levels and the pathological changes were analyzed. Antibody and cytokine levels were determined to explore the mechanisms involved. Results: Total and SHLI-specific IgE levels were significantly increased after SHLI sensitization. Systemic symptoms, local skin reactions, elevated histamine levels and decreased blood pressures were observed after challenge. Histological examination revealed that slight pathological changes occurred in lungs while no obvious alteration was found in intestine. IL-4 but not IFN-γ was significantly increased in spleen cells from SHLI-sensitized rats, indicating SHLI-induced anaphylaxis may be Th2-mediated. Conclusion: This rat model may provide a useful tool to study the mechanisms in SHLI-induced anaphylaxis and suggest some novel therapeutic approaches for this anaphylaxis.
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Objective: To explore the values for rejection research of the models through observing rejection rulers following small bowel transplantation(SBT)in Inbred strain rats.Mothods: Heterotopic SBT models were established with Inbred strain F344/RT11 and BN/RT1n by microsurgical technique according to Monchik method,including Isotransplantation(F344→F344,n=8)and allotransplantation(BN→F344, n=8).Results: 1.The mean living time was over 30 days in IT x group ,which was 12 days in AT x group.2.The general conditions have no significant difference on various time points of postoperative day(POD)in IT x group,while pathological features of grafts were concided with diagnosis of nonspecific slight inflammation on POD3 and were the same as the normal grafts on POD 0?5?7 and 9.3.Significant difference of IL-2R? chain and ?-INF mRNA transcription was earlier than pathological feature appearance of rejection.4.The general conditions and pathological features of grafts were concided with diagnosis of mild,moderate and severe actute rejection in AT x group on POD 5?7and 9 respectively.Conclusion:Inbred strain BN to F344 combination is fitter for research on rejection mechanism than F344 to Wistar combination and Outbred strain Wistar to SD combination.