ABSTRACT
Since the enactment of environmental protection Act in 1989 and Department of Biotechnology (DBT) guidelines to deal with genetically modified organisms, India has embarked on establishing various levels of biosafety laboratories to deal with highly infectious and pathogenic organisms. Occurrence of outbreaks due to rapidly spreading respiratory and haemorrhagic fever causing viruses has caused an urgency to create a safe laboratory environment. This has thus become a mandate, not only to protect laboratory workers, but also to protect the environment and community. In India, technology and science are progressing rapidly. Several BSL-3 [=high containment] laboratories are in the planning or execution phase, to tackle biosafety issues involved in handling highly infectious disease agents required for basic research and diagnosis. In most of the developing countries, the awareness about biocontainment has increased but planning, designing, constructing and operating BSL-3 laboratories need regular updates about the design and construction of facilities and clear definition of risk groups and their handling which should be in harmony with the latest international practices. This article describes the major steps involved in the process of construction of a BSL-3 laboratory in Indian settings, from freezing the concept of proposal to operationalization phase. The key to success of this kind of project is strong institutional commitment to biosafety norms, adequate fund availability, careful planning and designing, hiring good construction agency, monitoring by experienced consultancy agency and involvement of scientific and engineering personnel with biocontainment experience in the process.
ABSTRACT
Pathogens handled in a Biosafety Level 3 (BSL-3) containment laboratory pose significant risks to laboratory staff and the environment. It is therefore necessary to develop competency and proficiency among laboratory workers, and promote behaviors and practices that enhance safety through biosafety training. Following installation of our BSL-3 laboratory at the Center for Microbiology Research-Kenya Medical Research Institute, in 2006, a biosafety training program was developed to provide training on BSL-3 safety practices and procedures. The training program was developed based on the World Health Organization specifications, with adjustments to fit our research activities and biosafety needs. The program is composed of three phases namely; initial assessment, a training phase that includes theory and practicum, and final assessment. This article reports the content of our training program.
ABSTRACT
Pathogens handled in a Biosafety Level 3 (BSL-3) containment laboratory pose significant risks to laboratory staff and the environment. It is therefore necessary to develop competency and proficiency among laboratory workers and to promote appropriate behavior and practices that enhance safety through biosafety training. Following the installation of our BSL-3 laboratory at the Center for Microbiology Research-Kenya Medical Research Institute in 2006, a biosafety training program was developed to provide training on BSL-3 safety practices and procedures. The training program was developed based on World Health Organization specifications, with adjustments to fit our research activities and biosafety needs. The program is composed of three phases, namely initial assessment, a training phase including theory and a practicum, and a final assessment. This article reports the content of our training program.
ABSTRACT
The control system of the French standard mobile BSL-3 is introduced.The working mode and opening or closing sequence is analyzed.The power safety design,the pressure ladder control using transducer and temperature control method are especially introduced in detail.Advanced control technique is summarized and some defects used in China are provided.Technical support is provided for the using and maintaining of the lab.
ABSTRACT
Objective To discuss the effect of different negative pressure to directional airflow in the main room of BSL-3 laboratory.Methods The author combined the experiment and numerical simulation in the study.Results Under the condition that the air supply volume in the main room of BSL-3 laboratory is ensured invariable,negative pressure is a critical factor to effect air distribution in the lab.Conclusion The simulation result shows that a good form of directional airflow under the condition of-50pa can come into being,which offers some reference to the engineering design.