Species Identification of Umbilicariales from Seven Areas and Antioxidant and Anti-inflammatory Activities of Crude Polysaccharides / 中国药学杂志

OBJECTIVE: To identify seven species from Umbilicariales and study the chemical constituents, antioxidant and anti-inflammatory activities of crude products from the hot-water extraction. METHODS: The seven species from Umbilicariales were identified using ITS sequence analysis, and the seven crude polysaccharides were extracted from the species by water extraction method. RESULTS: Umbilicaria esculenta, Umbilicaria yunnana, Dermatocarpon miniatum and Lasallia papulosa were identified, and the main components of crude polysaccharides from the species were carbohydrates. The crude polysaccharides (JUY) from Umbilicaria yunnana of Jiangxi had the best antioxidant ability, it exhibited a higher scavenging activity of superoxide radicals (EC50=0.56 mg•mL-1) than Vc (EC50=1.67 mg•mL-1), and the scavenging activity for hydroxyl radicals (EC50=0.53 mg•mL-1) was comparable to Vc (EC50=0.51 mg•mL-1). Anti-inflammatory experimental results show that JUY has the best anti-inflammatory effects, and the better inhibitory effect of IL-1β than dexamethasone (P<0.01). CONCLUSION: The species from Umbilicariales of seven different areas were identified as four genera. The crude polysaccharides (JUY) from Umbilicaria yunnana of Jiangxi had the best antioxidant and anti-inflammatory activities.

Study of direct identification of pathogens from positive blood cultures by methods of separation gel tube -centrifugation / 中国基层医药

Objective To study the possibility of direct identification of pathogens from positive blood cul-tures by methods of separation gel tube -centrifugation.Methods 216 cases of positive blood culture were collected from 2015.7 to 2015.12.The bacterias were purified from blood culture bottle by separation gel tube.After washing 2 times,identified by MALDI -TOF MS.At the same time,traditional culture,smears and identification were done. Compared the results of identification by two methods.Results 216 cases of positive blood culture were single bacte-rial infection.By Gram stain,89 strains were Gram positive,119 strains were Gram negative and 8 strains were fungal spores.190 cases of positive blood culture were identified by MALDI -TOF MS,it concluded 67 Gram positive strains,111 Gram negative strains,4 anaerobe strains and 8 fungus.Compared with traditional culture,the coincidence rate reached up to 87.9%,Gram positive strains 78.8%,Gram negative strains 93.2%,anaerobe strains 100.0%and fungus 100.0%.Conclusion It takes less than 30 minutes purified from blood culture bottle by separation gel tube.And the time of identification is shorter than traditional culture.This method is good for clinical diagnosis and treatment.

Characterization of one strain of acid-fasting Propionibacterium isolated from the breast abscess samples / 国际检验医学杂志

Objective To identify one acid‐fasting bacteria isolated from wound secretion of breast abscess .Methods The acid‐fasting strain was identified by the morphological characteristics ,API 20A strips ,classical biochemical reaction ,and 16S rRNA gene sequencing .Results Cells of the strain was anaerobic ,non‐spore‐forming ,pleomorphic ,straight or curved rods ,which Gram and acid‐fast stain both were positive .After incubation for 5 days on sheep blood agar ,colonies were than 2 mm in diameter ,circular , smooth ,entire ,bump ,rice cream‐like withβ‐hemolysis .The 16S rRNA gene sequences were 100% identity to Propionibacterium av‐idum .The API 20A profile was 44365062 with positive Voges‐Proskauer test ,which was also consistent to Propionibacterium avi‐dum .Conclusion The pathogens of breast abscess is Proionibacterium avidum ,which is the first acid‐fasting Propionibacterium re‐ported in China .

Identification and renaming of one strain used for tracheitis vaccine production / 中华微生物学和免疫学杂志

Objective To identify and rename one strain stored in National Center for Medicine Culture Collections ( CMCC) and used for tracheitis vaccine production. Methods The test strain CMCC (B)29108 and the type strain DSM30007T were cultured on NA medium. Characteristics in morphology, physiology, biochemistry and fatty acid profile were compared between the two strains. Phylogenetic analysis was based on 16S rRNA and rpoB gene sequences, together with the DNA-DNA hybridization assay. Results A Comparative analysis of a partial sequence of the 16S rRNA gene sequence revealed that the CMCC( B) 29108 strain was closed to the Acinetobacter species and showed the highest similarity with the type strain Acinetobacter baumannii DSM30007T. Moreover, the CMCC(B)29108 strain was highly similar to type strain DSM30007T in morphology, physiology, biochemistry and fatty acid profile. On the phylogenetic tree based on 16S rRNA and rpoB gene of all Acinetobacter members, the CMCC(B)29108 strain steadily clustered into one independent branch only with the DSM30007 T strain with a DNA-DNA hybridization value of 100%. Conclusion The CMCC(B)29108 strain that is one of the strains used for the production of tracheitis vac-cine should be assigned to the species of Acinetobacter baumannii based on its phenotypic, phylogenetic and chemotaxonomic characteristics.

Phenotypic analysis of Pasteurella pneumotropica in laboratory animals in Beijing area / 中国比较医学杂志

Objective To improve the accuracy of detection through analyzing the phenotypes of P.pneumotropica isolates in laboratory animals in Beijing area .Methods 306 suspicious P.pneumotropica strains were identified by biochemical identification and 16S rDNA sequencing.Then, to obtain the phylogenetic relationships combined with colony characteristics on blood agar plates and biochemical characteristics of 53 biotypes .Results BD Phoenix 100 automated bacterial identification system and 16S rDNA sequencing identified P.pneumotropica positive rate of 306 isolates were 164/306 and 227/306, respectively.There were 140 phenotypes in 227 true-positive strains, of which 106 were biotype Heyl and 23 were biotype Jawetz .Conclusions In the samples of laboratory animals in Beijing area , P.pneumotropica infection mainly are of biotype Heyl , and less is of biotype Jawetz .The phenotypes are diverse and widely distributed .

Rapid Identification of Multiple Bacteria on a Microfluidic Chip / 分析化学

We developed a microfluidic device to integrate sample introduction, bacteria culturing and results reading. The identification of multiple bacteria was achieved by combining the spatial resolution of the arrayed bacteria culture chambers and the color resolution benefited from the bacteria specific chromogenic media. A set of 4 common pathogenic bacteria responsible for urinary tract infection were used as a model to test the microfluidic assay. Our results showed that the bacteria identification assay can be completed in 15 h, with a limit of detection (LOD) of bacteria density down to 10 cfu / mL. Clinical sample testing using the microchip approach showed a coincidence rate of 96. 3% as compared with the conventional method. The developed microfluidic approach is simple and rapid, thus hold the potential to serve as a powerful tool for detection of multiple bacteria.

Identification and Screening of Acid-tolerant LAB from Nomad Yogurt in Gobi Region of Mongolia / 微生物学通报

45 strains of Lactobacillus were isolated from 17 samples of traditional fermented milk in Gobi region of Mongolia. Based on morphological, physiological, biochemistry test and 16S rDNA sequencing analysis, identified as 31 strains of Lactobacillus fermentum(L. fermentum), 12 strains of L. helveticus, one strain of L. plantarum and one strain of L. casei. Survival rate of IMAU20085 is 81.44% in the screening experiment of resistance to the artificial gastric juice (pH 3.0). The isolation and identification of these strains and the screening of high acid-tolerant strains have important meaning to the preservation and exploitation of probiotic resource.

Development of a Rapid System for Bacteria Identification in 24 Hours / 中华医院感染学杂志

OBJECTIVE To develop a rapid system for bacteria identification and susceptibility test in 24 hours,and provide bacteriological evidence for the control of nosocomial infection and the timely diagnosis and treatment.METHODS The simple constant temperature box was replaced by revolving constant temperature box;2,3,5-triphenyltetrazolium chloride(TTC) and succinate were added to the culture bottle and the culture medium of drug susceptibility;the concentration of the reactive substrate in the bacterial biochemical tube and the number of the inoculated bacteria were increased at the same time.RESULTS The time of positive blood culture in the revolving constant temperature box was significantly shorter than that in the simple one(?2=74.92,P

Separating and Studying of the Aerobic Denitrifying Bacteria from Biofilter / 微生物学通报

Four strains of aerobic denitrifying bacteria were isolated from biomembrane of the laboratory biofilter by selective medium. The denitrifying rates of these four strains were found more than 80% under aerobic condition for 40 hours. The denitrifying rate of A1 was highest, which was 99.05%. When measuring the course of nitrogen element changing, it is found that they accumulated nitrite expect the strain A1. The strain A1 was gram positive and spherical. It is identified as Pseudomonas putida based on its biochemical and morphological characters and phylogenetic analysis of 16S rDNA sequences(genbank accession NO.DQ836052.1). For the strain A1, the optimum beginning pH was 7.0 around, and the optimum tempera- ture was 30 ℃ around, The change of DO did not influence the effect of denitrification when it was more than 2.0 mg/L.