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Objective: To confirm the influential factors on mycelial growth of Omphalia lapidescens and to provide a basis for the artificial cultivation. Methods: The mycelial growth rates of O. lapidescens under different culture conditions were measured. Results: With the temperature of 25 ℃ and pH 6.0-7.0, the mycelium of O. lapidescens was in its rapid growth. And there was no significant difference in mycelial growth rate with the concentration of carbon sources between 1% and 3%. Nitrogen sources such as peptone and yeast powder inhibited the mycelial growth, while the corn flour was not inhibited. The more suitable ingredient for the cultivation of O. lapidescens was bamboo leaves, meanwhile the growth-promoting effect of corn flour was obvious. Conclusion: The effects of temperature, nitrogen source, and matrix on mycelial growth of O. lapidescens are significant.
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Objective: To study the effective components of the leaves in six kinds of ground bamboo (Shibataea chinensis, Pleioblastus kongosanensis f. aureostriatus, Indocalamus latifolius, Sasa pygmaea, S. argenteastriatu, and S. veitchii) and their anti-oxidant activities. Methods: The contents of total phenolics, total flavonoids, and triterpenoids in bamboo leaves and their anti-oxidant activities were detected and investigated by using Forint reduction assay reagent, aluminum nitrate-sodium nitrite colorimetry, and vanillin-acetic acid assay, respectively. Eight characteristic compounds (orientin, isoorientin, vitexin, homovitexin, p-coumaric acid, chlorogenic acid, caffeic acid, and ferulic acid) were quantitatively analyzed by UPLC synchronously. The anti-oxidative and reducing activities of extracts from bamboo leaves were investigated by DPPH and FRAP. Results: Six kinds of ground bamboo leaves contained abundant botanic secondary metabolites. The content of total phenolics, on dry basis of leaves, varied in a range of 4.15%-9.12% in the six kinds of ground bamboo, total flavonoids and triterpenoids located in 1.62%-4.00% and 0.44%-0.57%, respectively. The sequence of the eight characteristic compounds in total phenolics was S. veitchii 6.81% > S. argenteastriatus 4.80% > S. pygmaea 4.35%, P. kongosanensis 4.35% > S. chinensis 1.54% > I. latifolius 1.08%. The content of eight characteristic compounds and their anti-oxidant activities in each ground bamboo leaves were significantly changed (P < 0.05). Conclusion: Among the six kinds of ground bamboo, there are much more effective components and more outstanding anti-oxidant activities of radical scavenging and iron reduction in two species (S. chinensis and P. kongosanensis) than those in any other banboos.
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Objective To study the antioxidant activity of the extract from north Ph.praecox leaves.Methods The extract from north Ph.Praecox leaves was made by ultrasonic-assisted extraction process,and the concentration of the total flavonoids was detected by NaNO3-Al(NO3)3-NaOH methods.The antioxidant capacity of the extraction was detected by DPPH free radical scavenging method and CuSO4-Phen-Vc-H2O2-DNA reaction with ultra-weak chemiluminescence method.Results The bamboo leaf extract could scavenges the DPPH free radical with the IC50 value as 7.02 mg /L and could delay the development of the emission peak value(P