ABSTRACT
OBJECTIVE:To establish a method for simultaneous determination of (R,S)-goitrin and chlorogenic acid in Banchai oral liquid.METHODS:The dual-wavelength HPLC method was adopted.The determination was performed on Wondasil C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid (11∶89,V/V) at the flow rate of 1.0 mL/min.The detection wavelengths were 245 nm for (R,S)-goitrin and 327 nm for chlomgenic acid.The column temperature was 30 ℃,and injection volume was 10 μL.RESULTS:The linear ranges were 4.05-40.51 μg/mL for (R,S)-goitrin (r=0.999 9),29.41-294.05 μg/mL for chlorogenic acid (r=0.999 9).The limits of quantification were 3.32,2.45 ng,limits of detection were 1.00,0.74 ng.RSDs of precision,stability and reproducibility tests were lower than 1.0％;the recoveries were 98.46％-101.06％ (RSD=0.98％,n=9) and 98.18％-100.78％ (RSD=0.86％,n=9).CONCLUSIONS:The method is simple,accurate,sensitive and reproducible,and can be used for simultaneous determination of (R,S)-goitrin and chlorogenic acid in Banchai oral liquid.
ABSTRACT
OBJECTIVE:To establish a method for simultaneous determination of (R,S)-goitrin and chlorogenic acid in Banchai oral liquid.METHODS:The dual-wavelength HPLC method was adopted.The determination was performed on Wondasil C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid (11∶89,V/V) at the flow rate of 1.0 mL/min.The detection wavelengths were 245 nm for (R,S)-goitrin and 327 nm for chlomgenic acid.The column temperature was 30 ℃,and injection volume was 10 μL.RESULTS:The linear ranges were 4.05-40.51 μg/mL for (R,S)-goitrin (r=0.999 9),29.41-294.05 μg/mL for chlorogenic acid (r=0.999 9).The limits of quantification were 3.32,2.45 ng,limits of detection were 1.00,0.74 ng.RSDs of precision,stability and reproducibility tests were lower than 1.0％;the recoveries were 98.46％-101.06％ (RSD=0.98％,n=9) and 98.18％-100.78％ (RSD=0.86％,n=9).CONCLUSIONS:The method is simple,accurate,sensitive and reproducible,and can be used for simultaneous determination of (R,S)-goitrin and chlorogenic acid in Banchai oral liquid.