ABSTRACT
The effect of Tujia medicine Berberidis Radix on endogenous metabolites in the serum and feces of mice with ulcerative colitis(UC) induced by dextran sulfate sodium(DSS) was analyzed by metabolomics technology to explore the metabolic pathway and underlying mechanism of Berberidis Radix in the intervention of UC. The UC model was induced in mice by DSS. Body weight, disease activity index(DAI), and colon length were recorded. The levels of tumor necrosis factor-α(TNF-α) and interleukin-10(IL-10) in colon tissues were determined by ELISA. The levels of endogenous metabolites in the serum and feces were detected by ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS). Principal component analysis(PCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA) were employed to characterize and screen differential metabolites. The potential metabolic pathways were analyzed by MetaboAnalyst 5.0. The results showed that Berberidis Radix could significantly improve the symptoms of UC mice and increase the level of the anti-inflammatory factor IL-10. A total of 56 and 43 differential metabolites were identified in the serum and feces, respectively, belonging to lipids, amino acids, fatty acids, etc. After the intervention by Berberidis Radix, the metabolic disorder gradually recovered. The involved metabolic pathways included biosynthesis of phenylalanine, tyrosine, and tryptophan, linoleic acid metabolism, phenylalanine metabolism, and glycerophospholipid metabolism. Berberidis Radix can alleviate the symptoms of mice with DSS-induced UC, and the mechanism may be closely related to the re-gulation of lipid metabolism, amino acid metabolism, and energy metabolism.
Subject(s)
Mice , Animals , Colitis, Ulcerative/drug therapy , Interleukin-10 , Metabolomics/methods , Chromatography, High Pressure LiquidABSTRACT
Objective To establish the quality evaluation system of Berberidis Radix (BR) standard decoction. Methods According to the preparation requirements of BR standard decoction, 15 batches of BR standard decoction powder was prepared, and, the index components in BR standard decoction was identified by using TLC chromatography. The content of index components was then detected by using HPLC, and the transfer rate of the index composition and the yield of the paste were calculated. Using infrared spectrometer to scan 15 batches of three standard decoctions. The infrared fingerprint spectrum was established, and the correlation between each batch was analyzed by using the two-index sequence analysis method. UV-vis spectrophotometer was used to respectively scan 15 batches of three needle standard medicinal broth for, establishing ultraviolet control map. The similarity between the fingerprint of sample and control was analyzed by SPSS software. Results The yield of 15 batches of standard decoction was 3.42%-6.36%. The content of berberine hydrochloride was 7.53%-13.98%, and the total transfer rate was 39.64%-73.62%. The same colored fluorescent spots were appeared at the corresponding positions of BR standard decoction, the berberine hydrochloride control, and BR control solution and the spots were in the same position of the same silica gel G thin layer. The lower limit of total peak rate of IR fingerprint was 50.0%, and the variation peak rate was 0.0%-62.5%. The similarity of UV fingerprints was all greater than 0.9, and the variation acceptable range was 0.768-1.000. Conclusion A stable and reliable method for the comprehensive quality evaluation of BR standard decoction was established, which can provide reference for the quality control of BR formula granules.
ABSTRACT
Sankezhen (Berberidis Radix) is a traditional Chinese materia medica,cold in nature and bitter in taste,for treating syndromes of liver,stomach,and large intestinal meridians,in which berberine and berbamine are the major pharmacological components.Sankezhen has been readmitted in Chinese Pharmacopoeia 2010 following the 1977 version as the roots of Berberis spp.e.g.B.soulieana,B.wilsonae,B.poiretii,B.vernae,etc.Recent studies showed that Berberis spp.were potential phytomedicines with multiple spectrums therapeutic effects and various pharmaceutical parts.Here we reviewed Sankezhen in traditional use and phytochemistry,and its major active components berberine and berbamine with potential bioactivities recently discovered,such as antitumor,antidiabetic,antihyperlipidemic,anti-arrhythmic,and neuro-protective activities.It is necessary to mature the quality assessment of Sankezhen as a new admission of Chinese Pharmacopoeia 2010.Other parts of Berberis spp.should be investigated to better develop this herb in medicinal usage.