ABSTRACT
To study the non-alkaloid constituents from the stems and leaves of Ochrosia borbonica. The chemical constituents were separated and purified by silica gel, Sephadex LH-20, ODS column chromatographies, and preparative HPLC. Their structures were determined by physicochemical properties, spectral data, as well as comparison with the data in literature. Fifteen compounds were isolated from the petroleum ether fraction of 70% ethanol extract from the stems and leaves of O. borbonica, and identified as lupeol (1), lupine ketone (2), betulinic alcohol (3), betulinic acid (4), ursolic acid (5), 2α, 3β-dihydroxy-ursolic acid (6), 11, 12-dehydroursolic acid lactone (7), β-amyrin (8), oleanolic lactone (9), alphitolic acid (10), friedelin (11), cholesterol (12), 24R-ethyl-5α-cholestane-3β, 6α-diol (13), stigmast-7-en-3β-ol (14), and β-sitosterol (15). All the compounds are isolated from the plants of Ochrosia Juss. for the first time.