ABSTRACT
Objective To study the changes of endotoxin in blood,Escherichia coli,lactobacillus,bifidobacterium,enterococci in feces of HCC patients.Methods Real-time fluorescence quantitative PCR was used to detect the difference between HCC group and normal group in the expression in bifidobacterium,lactobacillus,enterococcus and Escherichia coli,and the difference of endotoxin level.Results The expression levels of Escherichia coli and enterococcus in HCC group were significantly higher than those in normal control group (P＜ 0.05).Bifidobacterium and lactobacillus in primary liver cancer group significantly decreased compared with the normal group (P ＜ 0.05).The serum endotoxin level in HCC group was significantly higher than that in normal control group (P ＜ 0.05).Bifidobacterium and lactobacillus increased in HCC group.Enterococcus and escherichia coli decreased in HCC group.There was a significant positive correlation between endotoxin content and tumor number and tumor diameter in HCC patients.Conclusion Bifidobacterium,lactobacillus decreased,enterococcus and Escherichia coli increased in HCC patients.These changes along with the increase of endotoxin may relate to the development of HCC.
ABSTRACT
Objective To evaluate the promote effect of Danisco products Bifidobacterium Lactis Bi07 & Lactobacillus acidophilus NCFM on physical intestinal canal microbial eco-system.Methods A 3-week randomized trial was conducted in 400 healthy persons aged from 25 to 45 years,with a male to female ratio of 1∶1,they were randomly divided into 2 groups: placebo group and probiotics group.Each group takes the corresponding products 100 g twice everyday at 10 a.m.and 4 p.m respectively.Results Digestive system on microbial eco-system was effectively improved.The viable count of Bifidobacteria and Lactobacillus was significantly increased.The viable count of Clostridiumperfringen was significantly decreased.Conclusions Significant effectiveness on physical intestinal canal microbial ecosystem were proved by intake Danisco probiotics products 200 g per day for 21 days.