ABSTRACT
Objective To investigate the distribution of virulence genes and resistance genes in a group of Staphylococcus aureus clinical isolates.Methods Forty strains of Staphylococcus aureus isolated from Ningbo First Hospital during July and September 2013 were collected.Forty-two kinds of virulence genes and 11 kinds of resistance genes were analyzed by polymerase chain reaction (PCR),and binary typing were performed based on 10 classes of virulence genes and resistance gene mecA.Results Among 40 Staphylococcus aureus strains,5 (12.5％) were sensitive to penicillin,and 17 (42.5％) were sensitive to erythromycin; The sensitive rates to the remaining 15 antibiotics were all higher than 65.0％.The positive rates of adhesins,cytotoxins,capsular antigens,superantigens,serine proteases were 2.5％-100.0％; While map gene was not detected.Resistance genes to β-lactam,aminoglycoside,erythromycin,tetracycline,polymer disinfectant and antibacterial peptide were also positive with positive rates of 2.5％-37.5％.By binary typing,40 strains of Staphylococcus aureus were divided into 16 kinds of positive modes.At least 3 classes of virulence genes were positive in all strains,and 7 classes of virulence genes and resistance gene mecA were positive in strain No.36.Conclusion The phenotypes of antibiotic resistance are well correlated with genotypes in this group of Staphylococcus aureus isolates,which carry several virulence genes and resistance genes.