ABSTRACT
Objective To study the mechanism of bioadhesive materials(Chitosan,Carbomer and hydroxypropyl methyl cellulose(HPMC))on promoting panax notoginseng saponins(PNS)′s oral absorption by microarray.Methods The study was divided into four groups.PNS,PNS-chitosan,PNS-carbomer and PNS-HPMC,microarray were used to investigate the change of genes expression level affiliated to the solute carrier transporter(SLC)and the ATP-binding cassette(ABC)after transpor-ting across Caco-2 cell monolayer,to explore the mechanism of bioadhesive materials′effect on PNS′s absorption.Results Comparing with PNS group ，chitosan and carbomer could significantly increase gene expression level affiliated to SLC transporter ，chitosan could decrease multi-resistant genes and P-gp efflux genes expression level affiliated to ABC transporter . HPMC had no obvious effect on SLC and ABC transporter .Conclusion Chitosan and carbomer increase PNS′s oral due to genes change affiliated to SLC and ABC transporter that could promote absorption and inhibit efflux .The promotion mechanism of HPMC absorption was that it could prolong retention time on absorption site .
ABSTRACT
Aim: The aim of this study to develop the controlled delivery of combination drug(s) to periodontal pocket. Materials and Methods: In the present investigation mucoadhesive gel formulations were prepared using carboxy methylcellulose (CMC), methylcellulose (MC), hydroxyethylcellulose (HEC), polyvinylpirrolidone (PVP), polycarbophil (PC), and poloxamer. Each formulation was characterized in terms of polarizing light microscopy, gelation, gel melting, hardness, compressibility, adhesiveness, cohesiveness, syringeability, adhesion to a mucin disk, rheological studies, drug release, and antibacterial activities. Addition of CMC and PVP to the gel favored hexagonal phase formation. The gelation temperature was decreased linearly with an increasing concentration of drug(s), whereas, the melting temperature increased with the concentration of drug(s). Increasing the concentrations of each polymeric component significantly increased formulation hardness, compressibility, adhesiveness, mucoadhesion, and syringeability, yet a decreased cohesiveness. Increased time of contact between the formulation and mucin significantly increased the required force of detachment. Drug release from all formulations was non-diffusion controlled and significantly decreased as the concentration of the polymer was increased, due to the concomitant increased viscosity of the formulations and the swelling kinetics of PC, following contact with the dissolution fluid. Result: Antibacterial studies revealed that a gel with 30% HEC had a growth inhibition zone on agar with all three strains. Conclusion: Formulations containing HEC exhibited superior physical characteristics for improved drug delivery to the periodontal pocket and are now the subject of long-term clinical investigations.