ABSTRACT
Biochemical Reaction Engineering is a professional discipline with emphasis on both theory and practice. It is the core course in the curriculum provision for students majoring in bioengineering and plays an important role in the cultivation of professional talents. However, there are so much theory knowledge, such as formulas and deduction, that students showed poor efficiency in their study. Herein, to cater the objective command of innovative talents under new education background, and to improve the practical teaching efficiency and the students' enthusiasm as well as the practical innovation capability, the teaching team innovated and explored the teaching pattern, the teaching method as well as the teaching technologies. The teaching efficiency has been remarkably improved by introducing the virtual simulate technology, the micro-lecture, the case teaching pattern attempts and the scientific platform, which can be used as reference by other peers.
Subject(s)
Humans , Bioengineering , Curriculum , StudentsABSTRACT
A mathematical model of competition-type lateral flow immunoassay (LFIA) was developed to describe the dynamic process of LFIA.The competition-type LFIA was divided into two categories: TwA-competition-type LFIA and TnA-competition-type LFIA.On the basis of the developed model, the COMSOL software was exploited to simulate the dynamic process of LFIA.The simulation result demonstrated the relationships between the concentrations of substances on the test and control lines and the influence factors.In particular, the influence factors in the TwA-competition-type LFIA included the concentrations of target analyte A (0-20 mol/L) and reporter particle P (0.01-100 mol/L), and the position of the test line (5-20 mm).On the other hand, the influence factors in the TnA-competition-type LFIA included the concentrations of target analyte A (0-20 mol/L) and reporter particle P (0.01-100 mol/L), and the porosity.Experiment result showed that the developed model could be used to explore the influence of the parameters on the test results, and optimize the performance of LFIA.
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Objective To study the correlation between Candida strains and vulvovaginal candidiasis. Methods Two groups of Candida albicans strains were chosen. Strains of group A and group B were isolated from vaginal discharge of normal women and of patients with candidal vaginitis respectively. The two methods, AMS (automated microbiological detection and identification system) and amino black stain, were applied to detect two phenotypic parameters, biochemical reaction of the strains and secretory capacity of the proteinases respectively, and analogous analysis was performed then. Results There was a significant difference in the levels of adonitol (P
ABSTRACT
Objective To isolate and identify the pathogenic bacterial strain Myroides odoratus from municipal sewage in Zhenjiang city.Methods With selective medium,a pathogenic bacterial strain JD23 was isolated from the city sewage.Combining the modern molecular biology technology methods and bioinformatics methods,its taxonomic status was confirmed by 16S rDNA gene sequence homologous analysis and phylogenetic tree.The traditional experiments of physiology,biochemistry and morphology were also studied.Results Strain JD23 exhibited the highest levels(98%) of similarity to the Myroides odoratus M58777.2.Combining all the results of several methods and morphological observation,strain JD23 was finally identified as the bacterial classification Myroides odoratus.Conclusion There was a pathogenic microorganism,Myroides odoratus,in municipal sewage of Zhenjiang city.