ABSTRACT
Newborns and children with Down syndrome ( DS) often prensent with transient abnormal myelopoiesis ( TAM) and have an increased risk of acute leukaemia. Most children are able to spontaneously resolve, but early mortality is high, and recurrence of acute megakaryoblastic leukemia (AMKL) occur in about 20% infants within 4 years. Sympotomatic babies with TAM may benefit from low-dose cytarabine. Children with DS are more likely to develop AMKL. DS-AMKL is sensitive to cytarabine and anthracyclines, thus having a superior outcome when compared with non-DS AMKL Age, sex, white blood cell count and risk group are similar between DS-ALL and non-DS-ALL, but DS-ALL has a unique spectrum of sentinel cytogenetic lesions that influences treatment outcome. Risk-adjusted intensive chemotherapy protocols demonstrate a good survival.
ABSTRACT
PURPOSE: Rotator cuff disease is the most common shoulder disease. Rotator cuff tear, which is related to cuff tendon degeneration, is commonly encountered in clinical practice. MATERIALS AND METHODS: Knowledge about the biology of the normal rotator cuff is fundamental to understanding the pathophysiology of and degenerative processes in rotator cuff tendon tears. Furthermore, such basic knowledge provides a rationale for and facilitates the development of treatment modalities. RESULTS AND CONCLUSION: Therefore, we reviewed the biology of the normal rotator cuff tendon, theories to explain the pathophysiology of rotator cuff tendon tear, and current research on apoptosis of rotator cuff tenofibroblasts.
Subject(s)
Apoptosis , Biology , Population Characteristics , Rotator Cuff , Shoulder , TendonsABSTRACT
Objective To observe the influence of ascites in severe acute pancreatitis (SAP) on the function and viability of peritoneal macrophages in order to investigate the role of peritoneal macrophages in pathophysiological alteration and secondary pancreatic infection in SAP. Methods After the ascites of SAP models treated peritoneal macrophages for 1,3,6,12 and 24 hours in vitro, neutral red phagocytosis, cell viability and TNF secretion of peritoneal macrophages were determined respectively. Results The phagocytosis, cell viability and TNF secretion of macrophage all decreased with the treating time prolonged in the tests. Conclusions The ascites of SAP decreased the phagocytosis, viability and TNF secretion of peritoneal macrophages, and was one of the factors to promote secondary pancreatic and peripancreatic infection as well as bacterial translocation of gut.
ABSTRACT
Objective:To study the biologic characters of human foreskin fibroblasts(HFF)to lay the foundation for a long-term culture for human embryonic germ cells in vitro.Methods:The method of separating HFF was investigated.By immunocytochemical method,MTT and flow cytometry,we investigated the morphology and the purity of HFF,study the cell growth curve and cell cycle of HFF,and the effects of different concentrations of mitomycin on growth of HFF at different times.Results:Epidermis and dermis were more easily separated after overnight digestion(12~16 h)in 0.25% Trypsin at 4℃,compared with incubation for 2 h in 5% CO2 at 37℃.Vimentin was strongly positive stained with the purity of 95 percent.There was no obvious stranded period.The rapid proliferation period was in 1~6 d of cell growth curve.The platform stage began at 7 d.Majority of the cells were in G1/G0 phases.The cell cycle corresponded to cell growth curve.Mitomycin C(MMC)inhibited the proliferation of HFF at 12.5 ?g/ml over 2.5 h of MMC treatment.Conclusion:Its biological characters showed that HFF can be used as feeder layer for in-vitro cultured human embryonic germ cells。