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AIM: To investigate the application of biological amniotic membrane soaked in pirfenidone(PFD)and to evaluate its anti-scarring effect and toxic side effects on glaucoma model of rabbit eyes.METHODS: The right eyes of 72 healthy New Zealand white rabbits were randomly divided into 0.5%PFD+ biological amniotic membrane group, biological amniotic membrane group, mitomycin C(MMC)group and blank control group after the glaucoma model was established by anterior chamber injection of compound carbomer solution, and 18 rabbits in each group underwent trabeculectomy, in which the 0.5% PFD+ biological amniotic membrane group was placed with 0.5% PFD solution-soaked biological amniotic membrane under the scleral flap, and the biological amniotic membrane group was placed with normal saline-soaked rehydrated biological amniotic membrane under the scleral flap. In the MMC group, a cotton pad soaked in MMC was placed under the scleral flap for 3 min and immediately rinsed with normal saline, while the blank control group received no implant after the scleral flap was made. The intraocular pressure(IOP), filtration blebs, toxic side effects and complications were evaluated, and the histopathological changes in the filtration area were observed by hematoxylin-eosin(HE), Masson staining and immunohistochemical staining.RESULTS: The mean IOP at 14, 21 and 28 d after trabeculectomy were 0.5%PFD+ biological amniotic membrane group<MMC group<biological amniotic membrane group<blank control group(all P<0.05). At 28 d after trabeculectomy, 0.5%PFD+ biological amniotic membrane group had the best effect of anti-inflammatory hyperplasia and inhibition of collagen formation, the highest survival rate of filtration blebs, and the inflammatory reaction was mild.CONCLUSION: Biological amniotic membrane soaked in pirfenidone has more obvious anti-scarring effect on glaucoma model, with less toxic side effects and good safety.
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@#AIM: To probe into the treatment effects of P50 EX-PRESS drainage nail combined with biological amniotic membrane implantation in primary open angle glaucoma(POAG).<p>METHODS: Totally 96 cases of 96 eyes with POAG and who treated from March 2013 to January 2019 were selected as the research objects, and the patients were divided into the observation group and the control group by the random number table method, with 48 cases in each group. The control group was implanted with P50 EX-PRESS drainage nail only, while the observation group were treated with biological amniotic membrane implantation on the basis of the control group. Then, the changes of intraocular pressure, retinal nerve fiber thickness(RNFLT), complications and success rate of operation before operation and after the operation for 7d, 1mo and 3mo of two groups of patients were observed.<p>RESULTS: The proportion of functional filtering blebs after operation for 3mo in the observation group was significantly higher than that in the control group(<i>P</i><0.05). There was no difference in preoperative intraocular pressure and RNFLT between the two groups(<i>P</i>>0.05). The intraocular pressure and RNFLT after operation for 7d, 1mo and 3mo in the observation group were significantly lower than those in the control group(<i>P</i><0.05). The incidence of complications in the observation group and the control group were 12% and 25% respectively(<i>P</i>>0.05). The success rates of operation in observation group and control group were 96% and 75% respectively(<i>P</i><0.05).<p>CONCLUSION:The application of P50 EX-PRESS drainage nail combined with biological amniotic membrane implantation for POAG, which the clinical effects are satisfactory, and which can improve the condition of filtering blebs, reduce intraocular pressure and RNFLT, thus it has good safety and high success rate.
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AIM: To investigate the effect of scleral slap invagination and trabeculectomy combined with amniotic membrane implantation in the treatment of glaucoma.?METHODS:From May 2013 to June 2017, 98 cases (116 eyes ) with glaucoma were treated in our hospital. According to the random number table method, the patients were divided into two groups: the observation group of 45 cases (58 eyes) with scleral slap invagination surgery combined with amniotic membrane transplantation into operation, the control group of 53 cases ( 58 eyes ) with trabeculectomy combined with amniotic membrane implantation. Postoperatively patients were followed up for 3mo to observe IOP control;using MEN-C90 type blood rheology analyzer to detect the whole blood viscosity, plasma viscosity, hematocrit and blood viscosity changes, using the Doppler flow imaging ( CDFI) technique for the detection of ophthalmic artery end diastolic velocity ( EDV ) , RI, and the peak systolic velocity ( PSV ) and other hemodynamic parameters change.?RESULTS:One week and 3mo after the surgery, mean IOP of the observation group was 12. 18 ± 0. 98mmHg, 12. 23 ± 0. 94mmHg, the control group was 14. 49 ±1. 64mmHg, 14. 62 ± 1. 59mmHg, the difference was statistically significant (P<0. 05); IOP successful-control rate of observation group after operation was 81. 0％, more than that of the control group ( 61. 3％), the difference was statistically significant (P<0. 05). At 1wk and 3mo after operation high blood viscosity, plasma viscosity, hematocrit and blood viscosity of two groups decreased significantly compared with the preoperative (P<0. 05); those of the observation group were better than those of the control group (P<0. 05). At 1wk and 3mo after operation, EDV and PSV of the two groups increased significantly, RI decreased significantly, compared with the preoperative differences were statistically significant ( P < 0. 05 ), those of the observation group were better than those of the control group (P<0. 05).? CONCLUSION: The combination of scleral slap invagination and amniotic membrane implantation has a significant effect on glaucoma patients, the effect of intraocular pressure control is good, the postoperative complications are low, and the safety of the patients is high.
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@#AIM: To explore the application value of scleral adjustable sutures in trabeculectomy and scleral biological amniotic membrane implantation for angle closure glaucoma. <p>METHODS: The clinical data of 94 patients(106 eyes)with angle closure glaucoma who underwent trabeculectomy and scleral biological amniotic membrane implantation were analyzed retrospectively. According to scleral adjustable suture used, the subjects were divided into the application group(study group, 54 patients 60 eyes)and the non-application group(control group, 40 patients 46 eyes). The occurrence of early shallow anterior chamber, continuous shallow anterior chamber and related complications in 2wk after surgery was observed and compared between the two groups. The formation of functional filtration bleb in 3mo after surgery was analyzed, and the improvement of intraocular pressure before surgery and 1mo after surgery was recorded.<p>RESULTS: In 2wk after surgery, the incidence rates of early shallow anterior chamber and continuous shallow anterior chamber in the study group were significantly lower than those in the control group(<i>P</i><0.05), while there were no significant differences in the incidence rates of complications between the two groups(<i>P</i>>0.05). One month after surgery, the intraocular pressure of the two groups were significantly lower than that before surgery(<i>P</i><0.05), and the difference of intraocular pressure before and after surgery in the study group was significantly higher than the control group. Three months after surgery, the formation rate of functional filtration bleb in the study group was significantly higher than that in the control group(<i>P</i><0.05). <p>CONCLUSION: The application of scleral adjustable sutures in trabeculectomy and scleral biological amniotic membrane implantation can effectively reduce the risk of shallow anterior chamber, which is of positive significance in improving intraocular pressure and formation of functional bleb in patients with angle closure glaucoma.
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To observe the effectiveness and safety of auto-corneallimbus stem cell transplantation combined double biological amniotic membrane transplantation in the treatment of pterygium ●METHODS: The patients performed pterygium surgeries in our hospital between 2010 - 06 and 2013 - 12 were selected, according to different pterygium classification grading, different surgical methods were taken. Group A: autologous corneal limbus stem cell transplantation was conducted. Group B: autologous corneal limbus stem cell transplantation combined double biological amniotic membrane transplantation were conducted. The patients were followed up 6-36mo after operation, the postoperative recurrence rate was observed. ●RESULTS: A total of 149 cases with 155 eyes included in this study, including 75 eyes in group A with the application of autologous limbal stem cell transplantation: thin type grade 2, 10 eyes ( 1 eye recurred ), grade 3, 14 eyes ( 2 eyes recurred );Hypertrophic 17 eyes (2 eyes recurred); Recurrence type 21 eyes (3 eyes recurred); Recovered 68 eyes (8 eyes recurred, the total recurrence rate was 10. 67%); Group B: 80 eyes treated with autologous limbal stem cell transplantation combined double biological amniotic membrane transplantation, thin type above grade 3 and grade 4 ( no recurrence); Hypertrophic 21 eyes (1 eye recurred); Recurrence type 37 eyes (2 eyes recurred);Recovered 77 eyes (3 eyes recurred, the recurrence rate was 3. 75%). Two groups of hypertrophic type, recurrence is significant in reducing the recurrence rate (P ●CONCLUSlON: lt indicates that recurrence rates are different when adopting different ways of operations. Autologous corneal limbus stem cell transplantation is applicable for no root type, pterygium of thin type below grade 2; Autologous corneal limbus stem cell transplantation with double biological amniotic membrane transplantation is suitable for thin type above grade 2, hypertrophic type, recurrent type of pterygium, with low recurrence rate, so it is the ideal surgical method.
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Objective To investigate the clinical efficacy of biological amniotic membrane for otoscpic repair of traumatic perforation of tympanic membrane. Methods Otoscpic repair was performed on the patients with tympanic membrane perforation which was not healed for 6 months. The efficacy was observed postoperatively. Results The success rate of the procedure was 97.37%, except that one patient was not healing by first intention due to wound infection. Conclusions Biological amniotic membrane is definitely efficacious in the surgical repair of perforation of tympanic membrane. Otoscpic sugical repair is less invassive and has a higher success rate.
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Background: To observe the impact of application of bio‑amniotic membrane immersed in 5‑fluorouracil solution in trabeculectomy on the retina in a rabbit model. Materials and Methods: Healthy white New Zealand rabbits were randomly assigned into three groups with 20 in each group. Bio‑amniotic membranes of 4 × 5 mm immersed in either physiological saline/water for 10 min, or 25 mg/mL 5‑fluorouracil solution for 5 and 10 min, respectively, were applied on rabbit eyes during trabeculectomy. At 7, 14, 21, and 28 days of postoperation, five rabbits from each group were examined with electroretinogram (ERG). After being examined for eye pressure and bleb morphology, rabbits were sacrificed by air embolism and their retinas were collected and examined by transmission electron microscopy (TEM). In addition, 5‑fluorouracil amount in bio‑amniotic membranes was measured using high‑performance liquid chromatography. Results: Each bio‑amniotic membrane could absorb 59.004 μg and 75.828 μg 5‑fluorouracil after being immersed in 5‑fluorouracil solution for 5 and 10 min, respectively. Application of these bio‑amniotic membranes in trabeculectomy could promote the formation of well‑functioning bleb and maintain intraocular pressure, although it had no effect on retina structures as examined with ERG and TEM. Conclusion: Application of 5‑FU soaked bio‑amniotic membrane in rabbit eye trabeculectomy is effective and safe.