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Objective: To investigate the expression of ER, PR, HER-2, PCNA and P53 in breast cancer patients in Shanghai and the relevant clinical significance. Methods: Expressions of ER, PR, HER-2, PCNA and P53 in the breast cancer tissues of 544 patients in Shanghai were detected by immunohistochemistry methods. Statistical analysis was applied to analyze the relationship of these immunohistochemical indices with the clinicopathological features of breast cancer. Results: The positive rates of ER, PR, HER-2, PCNA and P53 in the breast cancer tissues of 544 patients were 62.2%, 57.2%, 15.1%, 82.6% and 58.5%, respectively. The expressions of ER, PCNA and P53 were correlated with the tumor size (P<0.05, P<0.01). The expressions of HER-2 and PCNA were correlated with axillary lymphatic metastasis (P<0.05, P<0.01). ER expression was positively correlated with PR expression(r=0.452, P=0.000) and PR expression was positively correlated with P53 expression (r=0.520, P=0.03). The 123 (22.6%) patients with triple-negative breast cancer (TNBC) had a higher axillary lymphatic positive rate than patients with non-TNBC (P<0.01). Co-expression of HER-2 with PCNA and co-expression of HER-2 with P53 were positively correlated with lymphatic metastasis (P<0.05). Conclusion: Population aging has a influence on the immunohistochemical characteristics of breast cancer patients patients in Shanghai. Combined examination of ER, PR, HER-2, PCNA and P53 is of clinical significance in the diagnosis, treatment, and prognosis prediction of breast cancer patients.
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Objective To analyze the correlation between 18F-FDG SUV and immunohistochemical index including GLUT1, Ki-67, MVD, survivin and cyclinA in non-snall-cell lung cancer. Methods Thirty-three patients with NSCLC underwent preoperative PET/CT examination and surgical operation. All patients were divided into two groups according to the size of tumor (cutoff=3 cm), metastasis of mediastinal or hilar lymph nodes or not, and histological types of the cancer, respectively. The expression of GLUT1, Ki-67, MVD, survivin and cyclinA were estimated with SP immunohistochemical technique, and were analyzed statistically to reveal the correlation to FDG SUV. Results The rate of positive expression of GLUT1, Ki-67 and CD34 were 66.67%, 72.73% and 100%, respectively. The mean value of CD34 in all 33 patients was 12.6±2.9 (12-56). The rate of positive expression of survivin was 84.85%, and the corresponding data of cyclinA was 27.27%. Conclusion There is linear correlation between FDG PET SUV and GLUT1, but not between FDG PET SUV and Ki-67, MVD, survivin and cyclinA. The expressions of GLUT1, Ki-67, MVD, survivin and cyclinA are not related with the size of tumor, nor metastasis of lymph nodes. The expression of GLUT1 and Ki-67 is related with histological types of the cancer, but not with MVD, survivin and cyclinA.
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Objective To investigate the expression of ER,PR,HER-2,PCNA and P53 in breast cancer patients in Shanghai and the relevant clinical significance.Methods Expressions of ER,PR,HER-2,PCNA and P53 in the breast cancer tissues of 544 patients in Shanghai were detected by immunohistochemistry methods.Statistical analysis was applied to analyze the relationship of these immunohistochemical indices with the clinicopathological features of breast cancer.Results The positive rates of ER,PR,HER-2,PCNA and P53 in the breast cancer tissues of 544 patients were 62.2%,57.2%,15.1%,82.6% and 58.5%,respectively.The expressions of ER,PCNA and P53 were correlated with the tumor size(P
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In order to study the correlation of ambient toluene and xylene exposure with their biological monitoring indices, we measured the concentration of toluene and xylene in the workplace. We also measured their biological monitoring indices of workers from August to November in 1994. The exposed group consisted of 103 male workers and the non-exposed group consisted of 34 male workers. The ambient concentration of toluene was 44.7 +/-55.4 ppm and that of xylene was 2.35+/-2.15 ppm. The urinary concentration of hippuric acid in exposed group was 1.72+/-1.53 g/g creatinine and that of non-exposed group was 0.34+/-0.28 g/g creatinine. The difference was statistically significant between two groups (p0.05). When compensated with urinary creatinine, the correlation coefficient of ambient toluene with urinary hippuric acid and ocresol were 0.63(p=0.0001) and 0.65(p=0.0001), respectively. When compensated with urinary specific gravity, the correlation coefficient of ambient toluene with hippuric acid and ocresol were 0.525 (p=0.0001) and 0.547 (p=0. 0001), respectively. The compensation method using urinary creatinine provided a higher correlation coefficient. We could not find any statistically significant differences between the duration of work and other variables (urinary hippuric acid, o-cresol and methylhippuric acid). In order to monitor biological indices of toluene-exposed workers, we suggest the measurement of urinary hippuric acid rather than o-cresol. As the compensation method, we suggest to use urinary creatinine rather than urinary specific gravity.